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القاء واکنش دفاعی پرتقال به کپک آبی (Pencillium italicum) با استفاده از اسانس گیاه کک‌گریز (Francoeuria undulate (L.) Lack) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 34، شماره 3 - شماره پیاپی 89، مرداد و شهریور 1397، صفحه 430-442 اصل مقاله (229.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.115025.2114 | ||
| نویسندگان | ||
| محبوبه‌السادات حسینی1؛ صدیقه محمدی* 2؛ علی رضا افتخاریان جهرمی2 | ||
| 1دانشجوی کارشناسی ارشد، گروه باغبانی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| 2استادیار، گروه بیماری‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| چکیده | ||
| پوسیدگی کپک آبی از مهمترین بیماری‌ها پس از برداشت میوه مرکبات است که توسط قارچ Pencillium italicum ایجاد و سالیانه منجر به از بین رفتن مقادیر زیادی از محصولات مرکبات می‌شود. اسانس گیاه کک‌گریز (Francoeuria undulate (L.) Lack) دارای خاصیت ضدباکتریایی بالایی بر میکروارگانیسم‌ها است. بدین‌منظور در دو آزمایش جداگانه، تأثیر غلظت‌های مختلف اسانس (چهار سطح: صفر، 400، 800 و 1600) گیاه مذکور در القاء مقاومت میوه پرتقال در برابر کپک آبی (دو سطح: بدون بیمارگر و بیمارگر) با بررسی آنزیم‌های دفاعی انجام شد. این آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام گردید. در آزمایش دوم اثر اسانس گیاهان بر کنترل بیمارگر در چهار غلظت 0، 400، 800 و 1600 در قالب طرح کاملاً تصادفی انجام شد. نتایج تجزیه واریانس نشان داد که اثر متقابل غلظت×بیمارگر در دو بازه زمانی 48 و 96 ساعت در سه صفت آنزیم کاتالاز، پراکسیداز و فنول معنی‌دار گردید. همچنین نتایج تجزیه واریانس نشان داد که اثر مستقل غلظت اسانس گیاه کک‌گریز بر درصد بازدارندگی از رشد بیمارگر بر روی میوه معنی‌دار گردید. تأثیر متقابل بیمارگر× نوع گیاه نشان داد که بیشترین میزان فنول، پراکسیداز و کاتالاز مربوط به تیمارهای سالم بود اما در تیمارهای بیمار همراه کک‌گریز میزان بالای فنول مشاهده شد. تأثیر متقابل وجود و عدم وجود بیمارگر× غلظت نشان داد که بیشترین میزان فنول در تیمارهای سالم در غلظت صفر و ppm800 بود. پراکسیداز نیز در تیمارهای سالم بیشتر بود اما میزان کاتالاز در تیمارهای مختلف تفاوتی نداشت. تأثیر متقابل نوع گیاه- غلظت اسانس نشان داد که اختلاف آماری بین تیمارهای مختلف در میزان فنول، پراکسیداز و کاتالاز نبود. | ||
| کلیدواژه‌ها | ||
| مقاومت؛ کک‌گریز (Francoeuria undulate (L.) Lack)؛ اسانس؛ کپک آبی (Pencillium italicum) | ||
| عنوان مقاله [English] | ||
| Inducing resistance reaction of orange against blue mold Pencillium italicum using the essential oil of Francoeuria undulata (L.) Lack | ||
| نویسندگان [English] | ||
| M.S. Hosseini1؛ S. Mohammadi2؛ A.R. Eftekhariyan Jahromi2 | ||
| 1M.Sc. student, Department of Horticultural Science, Shiraz Branch, Science Islamic Azad University, Shiraz, Iran | ||
| 2Department of Plant Pathology, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| چکیده [English] | ||
| Mold decay is one of the most important diseases after harvesting citrus fruits, produced by Pencillium italicum fungi. It annually causes high amount of losses in citrus products. The essential oil of coca bush, Francoeuria undulata (L.) Lack, has high antibacterial properties on microorganisms. Therefore, in two separate experiments, the effect of various concentrations of essential oils (four levels including 0, 400, 800 and 1600) on inducing resistance of orange's fruit was evaluated against blue mold (two levels: without pathogen and with pathogen) via studying the defense enzymes. The present study was conducted as factorial based on completely randomized design with three replications. In second experiment, the effect of essential oil on pathogen control was studied at concentrations of 0, 400, 800, and 1600 in a completely randomized design. Results of analysis of variance showed that the interaction of concentration×pathogen at 48 and 96- hour time periods was significant on three enzymes including catalase, peroxidase and phenol. Also, the results of analysis of variance showed that the effect of coca bush essential oil concentration on inhibitory of pathogen growth on fruit was significant. The interaction of pathogen× plant type showed that the highest content of phenol, peroxidase and catalase was associated with control treatments, while high phenol content was observed in pathogenic treatments with coca bush. The interaction of presence and absence of pathogen×concentration showed that the highest phenol content was obserevd in control treatments at 0 and 800 ppm concentrations. Peroxidase content was high in healthy treatments, while there was no difference among various treatments in terms of catalase content. The interaction of plant type×essential oil concentration showed that there was no significant difference among various treatments in terms of phenol, peroxidase and catalase content. | ||
| کلیدواژه‌ها [English] | ||
| resistance, coca bush (Francoeuria undulata (L.) Lack), essential oil, Pencillium italicum | ||
| مراجع | ||
|
- Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2): 265-267. - Aebi, H., 1984. [13] Catalase in vitro. Methods in Enzymology, 105: 121-126. - Agrios, G., 2005. Plant Pathology. Dana Dreibelbis, 24p. - Alpsoy, L., 2010. Inhibitory effect of essential oil on aflatoxin activities. African Journal of Biotechnology, 9(17): 2474-2481. - Amiri, H., Lari Yazdi, H., Dusti, B. and Samsam Nia, F., 2011. Essential oil composition and anatomical study of Oliveriadecumbens vent. Journal of Medicinal and Aromatic Plants, 26(4): 513-520. - Arras, G. and Usai, M., 2001. Fungitoxic activity of 12 essential oils against four postharvest citrus pathogens: chemical analysis of Thymus capitatus oil and its effect in subatmospheric pressure conditions. Journal of Food Protection, 64(7): 1025-1029. - Bakkali, F., Averbeck, S., Averbeck, D. and Idaomar, M., 2008. Biological effects of essential oils-a review. Food and Chemical Toxicology, 46(2): 446-475. - Barnet, H.L. and Hunter, B.B., 1998. Illustrated Genera of Imperfect Fungi. The American Phytopathological Society, Minnesota, 218p. - Bowler, C., Montagu, M.V. and Inze, D., 1992. Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology, 43(1): 83-116. - Daly, J.M., Ludden, P. and Seevers, P., 1971. Biochemical comparisons of resistance to wheat stem rust disease controlled by the Sr6 or Sr11 alleles. Physiological Plant Pathology, 1(4): - De Gara, L., de Pinto, M.C. and Tommasi, F., 2003. The antioxidant systems vis-à-vis reactive oxygen species during plant-pathogen interaction. Plant Physiology and Biochemistry, 41(10): 863-870. - Deepak, S., Niranjan-Raj, S., Shailasree, S., Kini, R.K., Boland, W., Shetty, H.S. and Mithöfer, A., 2007. Induction of resistance against downy mildew pathogen in pearl millet by a synthetic jasmonate analogon. Physiological and Molecular Plant Pathology, 71(1): 96-105. - Du, Y.Y., Wang, P.C., Chen, J. and Song, C.P., 2008. Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana. Journal of Integrative Plant Biology, 50(10): 1318-1326. - Ghahraman, A., 1994. Iranian Color Flora,. Tehran University, 73-1357. - Hammer, K.A., Carson, C. and Riley, T., 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6): 985-990. - Javadinamin, A. and Asgarpanah, J., 2014. Essential oil composition of Francoeuria undulata (L.) Lack. growing wild in Iran. Journal of Essential Oil Bearing Plants, 17(5): 875-879. - Jayakannan, M., Bose, J., Babourina, O., Rengel, Z. and Shabala, S., 2015. Salicylic acid in plant salinity stress signalling and tolerance. Plant Growth Regulation, 76(1): 25-40. - Kuć, J., 2001. Concepts and direction of induced systemic resistance in plants and its application. European Journal of Plant Pathology, 107(1): 7-12. - Larrigaudiere, C., Vilaplana, R., Soria, Y. and Recasens, I., 2004. Oxidative behaviour of Blanquilla pears treated with 1-methylcyclopropene during cold storage. Journal of the Science of Food and Agriculture, 84(14): 1871-1877. - Mahboubi, M., Feizabadi, M.M., Haghi, G. and Hosseini, H., 2008. Antimicrobial activity and chemical composition of Oliveria decumbens Vent. Iranian Journal of Medicinal and Aromatic Plants, 24(1): 56-65. - Mahmoud, A.M., El-Baky, R.M.A., Ahmed, A.B.F. and Gad, G.F.M., 2016. Antibacterial activity of essential oils and in combination with some standard antimicrobials against different pathogens isolated from some clinical specimens. American Journal of Microbiological Research, 4(1): 16-25. - Mayr, U., Batzdorfer, R., Treutter, D. and Feucht, W., 1993. Surfactant-induced changes in phenol content of apple leaves and fruit skins. Paper Presented at the International Symposium on Natural Phenols in Plant Resistance, 381: 479-487. - Plaza, P., Sanbruno, A., Usall, J., Lamarca, N., Torres, R., Pons, J. and Viñas, I., 2004. Integration of curing treatments with degreening to control the main postharvest diseases of clementine mandarins. Postharvest Biology and Technology, 34(1): 29-37. - Prusky, D., McEvoy, J.L., Saftner, R., Conway, W.S. and Jones, R., 2004. Relationship between host acidification and virulence of Penicillium spp. on apple and citrus fruit. Phytopathology, 94(1): 44-51. - Rahemi, M., 2003. Post-harvest physiology: Introduction to physiology and the displacement of fruits, vegetables and ornamental plants. Shiraz University Press, 437p. - Singh, S., Das, S., Singh, G., Schuff, C., de Lampasona, M.P. and Catalán, C.A., 2014. Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.). BioMed Research International, 1-10. - Swamy, M.K. and Sinniah, U.R., 2015. A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: an aromatic medicinal plant of industrial importance. Molecules, 20(5): 8521-8547. - Tripathi, P. and Dubey, N., 2004. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biology and Technology, 32(3): - Vero, S., Mondino, P., Burgueno, J., Soubes, M. and Wisniewski, M., 2002. Characterization of biocontrol activity of two yeast strains from Uruguay against blue mold of apple. Postharvest Biology and Technology, 26(1): 91-98. - Walker, G.M., Mcleod, A.H. and Hodgson, V.J., 1995. - Wang, Y.S., Tian, S.P., Xu, Y., Qin, G.Z. and Yao, H., 2004. Changes in the activities of pro-and anti-oxidant enzymes in peach fruit inoculated with Cryptococcus laurentii or Penicillium expansum at 0 or 20C. Postharvest Biology and Technology, 34(1): 21-28. - Willekens, H., Chamnongpol, S., Davey, M., Schraudner, M., Langebartels, C., Van Montagu, M. and Van Camp, W., 1997. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. The EMBO Journal, 16(16): 4806-4816. - Zakerian, A.S.R., Aboutalebi, A.S. and Safi Zadeh, M.R., 2011. Effect of extracts of medicinal plants and clay on post-harvest quality of Novel oranges. The first National Conference on Modern Topics in Agriculture, Saveh Branch, Science Islamic Azad University, Saveh, 8 November. | ||
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