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Vitis Elegan as a Promising Antidiabetic Herb: Phytochemical and Pharmacological Assessment | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 9، دوره 7، شماره 1، تیر 2018، صفحه 67-73 اصل مقاله (143.54 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2018.116730 | ||
| نویسندگان | ||
| Fatemeh Sadeghi1؛ Jamaludin Bin Mohamad2؛ Samad Muhammadnejad3؛ Mahnaz Haddadi4؛ Mohammad Shirkhoda5؛ Sepideh Mehrzad6؛ Tahereh Barati* 7 | ||
| 1Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia | ||
| 3Research Center for Molecular and Cellular Imaging, Tehran University of Medical sciences, Tehran, Iran | ||
| 4Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Science, Tehran, Iran | ||
| 5Department of Surgical Oncology, Cancer Institute of Iran, Tehran University of Medical Science, Tehran, Iran | ||
| 6Department of Genetic, Central Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 7Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| In this research, we investigated the antidiabetic activity of Vitis elegans rhizome, which is used as traditional treatment for diabetes mellitus. The methanol, chloroform, petroleum ether, and hexane extracts of the plant root were obtained through serial exhaustive extraction and were analyzed by Thin Layer Chromatography (TLC). Glycogen phosphorylase (GP) assay was done to determine the inhibitory effects of respective extracts on GP enzyme. Total phenol content was measured using the Folin-Ciocalteu method and brine shrimp test was done to evaluate the toxicity of the extracts. Evaluation of TLC plates alone and after spraying with different reagents indicated that terpenoid was the major component of the sample followed by alkaloid and phenol. Chloroform extract applied more inhibitory effects on GP enzyme activity with percentages of 39.65 in concentration of 2.5 mg/ml. This suppression effect was higher than glucose, as a standard inhibitory agent in the body. The highest amount of phenol was found in the methanol extract, equal to 49 mg GAE g-1. Petroleum ether, chloroform and methanol extracts were considered as non-toxic solvents with LC50 values of 8.9, 3.5 and 3.7 mg/ml respectively. While hexane with 0.089 mg/ml LC50 value was classified as toxic extract. Based on the results of this study, we concluded that Vitis elegans rhizome, has the potential to be further studied for anti-hyperglycemic properties. | ||
| کلیدواژهها | ||
| Anti-diabetic؛ Enzyme Inhibition؛ Glycogen phosphorylase؛ Phytochemical screening؛ Vitis elegans | ||
| مراجع | ||
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