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Application of gene transfer technology in aquaculture | ||
| علوم آبزی پروری پیشرفته | ||
| Article 3, Volume 2, spring, June 2018, Pages 39-54 PDF (714.64 K) | ||
| Document Type: article | ||
| Authors | ||
| shirin jamshidi* 1; samira nazem roaya2 | ||
| 1Agricultural Biotechnology Research Institute of Iran, Gilan Branch, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. | ||
| 2South Iran Aquaculture Research center, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahwaz, Iran | ||
| Abstract | ||
| Aquatic transgenic organism is an organism with a foreign gene or non-coding deoxyribonucleic acid (DNA) fragment is artificially introduced and stably integrated in their genomes. Since the first report in 1985, a wide range of transgenic fish and marine bivalve mollusks and crustaceans have been produced by microinjecting or electroporating homologous or heterologous transgenes into newly fertilized or unfertilized eggs and sperm. For producing transgenic organism many factors has been included. The first and the most important factor is aquatic species for gene transfer. In species selecting for gene transfer the target of research and species preservation with a high safety conditions and facilities should be concerned. The second factor is construct of target gene should be programmed for make it and need for these studies. For example gene construct in gene transfer method should have open reading frame of gene target. Also gene construct should have regulatory factors for regulating stable or transient gene expression or affect growth and development in organism. In the third step, recombinant construct should be transfer into sperm or embryo for stable gene expression. In the end stage, individuals received target gene should be traced. in addition, application of transgenic technology for producing transgenic fish and other aquatic organisms with beneficial traits such as somatic body growth, resistance to diseases and producing useful product for human, domesticated animal, poultry and aquatic organisms have been increased by biotechnology. | ||
| Keywords | ||
| Aquatic transgenic organisms; gene transfer; construct; transgenic fish; biotechnology | ||
| References | ||
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