| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,230 |
| تعداد مقالات | 35,784 |
| تعداد مشاهده مقاله | 54,012,384 |
| تعداد دریافت فایل اصل مقاله | 93,170,562 |
Prevalence of Canine Monocytic Ehrlichiosis in Canine Population Across India | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 73، شماره 2، شهریور 2018، صفحه 87-93 اصل مقاله (271.35 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116616 | ||
| نویسندگان | ||
| K. Kukreti1؛ L. Pandey* 2؛ M. Das3؛ A. Rastogi3؛ R. Dubey3؛ P. Sharma3 | ||
| 1Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh | ||
| 2St. aloysius College, Jabalpur (MP), India | ||
| 3Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, 250001, India | ||
| چکیده | ||
| Canine ehrlichiosis is a very important emerging disease in India. This study is the first attempt screening a large number of canines in India for the detection of canine monocytic ehrlichiosis. In the present study, 510 blood samples of dogs were screened for the presence of Ehrlichia canis and other variants of Anaplasmataceae family by serological and molecular methods.Out of the 510 serum samples, 293 (57.5%) cases were found positive for the presence of E. canis antibodies through enzyme-linked immunosorbent assay (ELISA). Furthermore, and 45 (8.8%) and 1 (0.2%) specimens were positive for E. canis and A. platys, respectively, based on the polymerase chain reaction (PCR). In the clinical samples of E. canis, the minimum detection limit for PCR was9 ng. In the immunofluorescence assay (IFA), the positive blood samples showed comparable results with those obtained from the commercially available dot ELISA kit (giving equivalent IFA titer). The results of sequencing were compared with other reported isolates in various regions of the world, and a phylogenetic relationship was established. The 16S rRNA region that was amplified and sequenced for E. canis and A. platys was highly conserved and so was another Vir B9 region. | ||
| کلیدواژهها | ||
| Ehrlichiosis canis؛ Anaplasma platys؛ Polymerase Chain Reaction؛ ELISA؛ India | ||
| عنوان مقاله [English] | ||
| مطالعه شیوع عفونت مونوسیتی سگان ارلی شیوز در جمعیتهای سگان در هند | ||
| نویسندگان [English] | ||
| Kartikaye Kukreti1؛ Laxmi Pandey2؛ Manoj Das3؛ Amit Rastogi3؛ Rahul Dubey3؛ Prashant Sharma3 | ||
| 1Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh | ||
| 2St. aloysius College, Jabalpur (MP), India | ||
| 3Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, 250001, India | ||
| چکیده [English] | ||
| به دلیل خارجی بودن نویسنده مقاله فاقد چکیده فارسی است. | ||
| کلیدواژهها [English] | ||
| به دلیل خارجی بودن نویسنده مقاله فاقد کلید واژه فارسی است | ||
| مراجع | ||
|
De Groot, A. S., Bosma, A., Chinai, N., Frost, J., Jesdale, B. M., Gonzalez, M. A., Martin, W., Saint-Aubin, C., 2001. From genome to vaccine: in silico predictions, ex vivo verification. Vaccines19, 4385-4395.
Donatien, A., F, 1935. Existence en Algerie d’une rickettsia du. Chien Bull Soc Pathol Exot 28, 418-419.
Engvall, E. O., Pettersson, B., Persson, M., Artursson, K., Johanssen, K.L., 1996. A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses and cattle. J Clin Microbiol 34, 2170-2174.
Felek, S., Greene, R., Rikihisa, Y., 2003. Transcriptional analysis of p30 major outer membrane gene of Ehrlichia canis in naturally infected ticks and sequence analysis of p30-10 of E. canis from diverse geographic regions. J Clin Microbiol 41, 886-888.
Goldman, E.E., Breitschwerdt, E.B., Grindem, C.B., Hegarty, B.C., Walls, J.J., Dumler, J.S., 1998. Granulocytic ehrlichiosis in dogs from North Carolina and Virginia. J Vet Intern Med12, 61-70.
Harrus, S., Waner, T., Alzenberg, I., Foley, J.E., Poland, A.M., Bark, H., 1998. Amplification of ehrlichial DNA from dogs 34 months after experimental infection with E. canis. J Clin Microbiol 36, 73-76.
Inokuma, H., Raoult, D., Brouqui, P., 2000. Detection of Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) in Okinawa island, Japan. J Clin Microbiol 38, 4219-4221.
Iqbal, Z., Chaichanasiriwthaya, W., Rikihisa, Y., 1994. Comparison of PCR with other tests for early diagnosis of canine ehrlichiosis. J Clin Microbiol 32, 1658-1662.
Kledmanee, K., Suwanpakdee, S., Krajangwong, S., Chatsiriwech, J., Suksai, P., Suwannachat, P.,et al., 2009. Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp. and Hepatozoan canis in canine blood. Southeast. Asian. J Trop Med Public Health40, 35- 39.
Kordick, S.K., Breitschwerdt, E.B., Hegarty, B.C., Southwick, K.L., Colitz, C.M., Hancock, S.I.,et al.,1999. Coinfection with multiple tick borne pathogens in a walker hound kennel in North Carolina. J Clin Microbiol 37, 2631-2638.
McBride, J.W., Corstvet, R.E., Gaunt, S.D., Chirsangarman, J., Akita, G.Y., Osburn, B.I., 1996. PCR detection of acute E. canis infection in dogs. J Vet Diagn Invest 8, 441-447.
Neer, T.M., Breitschwerdt, E.B., Greene, R.T., Lappin, M.R., 2002. Consensus statement on ehrlichial disease of small animals from the infectious disease study group of the ACVIM. J Vet Intern Med 16, 309-315.
Ristic, M., Huxol, D.L., Weisiger, R.M., Hildebrandt, P.K., Nyindo, M.B.A., 1972. Serological diagnosis of tropical canine pancytopenia by indirect immunoflouresence. Infect Immun 6, 226-231.
Saunders, N.J., Moxon E.R., 1998. Implications of sequencing bacterial genomes for pathogenesis and vaccine development. Curr. Opin Biotechnol 9,618- 623.
Unver, A., Perez, M., Orellana, N., Huang, H., Rikihisa, Y., 2001. Molecular and antigenic comparison of Ehrlichia canis isolates from dogs, ticks, and a human in Venezuela. J Clin Microbiol 39, 2788-2793.
Wen B., Rikihisa, Y., Mott, J. M., Green, R., Kim, H. Y., Zhi, N.,et al., 1997. Comparison of nested PCR with immunoflourescent antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. J Clin Microbiol 35, 1852-1855.
Woody, B. J., J. D. Hoskins., 1991. Ehrlichial diseases of dogs. Vet Clin N Am: Small Anim Pract 21, 75-98. | ||
|
آمار تعداد مشاهده مقاله: 2,493 تعداد دریافت فایل اصل مقاله: 3,735 |
||