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پاسخ مؤلفه های رویشی و فیزیولوژیکی گیاه گندم به شوری و تأثیر پیش تیمار زیستی بذر با قارچ های Piriformospora indica و Trichoderma virens در بهبود سازگاری گیاه به تنش شوری | ||
| علوم و فناوری بذر ایران | ||
| مقاله 7، دوره 6، شماره 2 - شماره پیاپی 12، آبان 1396، صفحه 77-90 اصل مقاله (954.11 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.116529 | ||
| نویسندگان | ||
| فهیمه سراج1؛ ندا سلیمی تملی1؛ همت اله پیردشتی* 2؛ یاسر یعقوبیان3 | ||
| 1دانشجوی کارشناسی ارشد/پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، مازندران. | ||
| 2دانشیار/پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، مازندران | ||
| 3دکتری زراعت/ دانشگاه کشاورزی و منابع طبیعی رامین خوزستان | ||
| چکیده | ||
| به منظور بررسی روند پاسخ صفات رویشی و فیزیولوژیک گندم رقم مروارید به پیش تیمار زیستی قارچهای Piriformospora indica(Pi وTrichoderma virens(Trich، در شرایط شور،آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار به اجرا درآمد. عامل های آزمایش شامل هفت سطح شوری (صفر، 40، 80، 120، 160، 200، 240 میلی مولار NaCl) و پیش تیمار زیستی قارچی شامل چهار سطح (عدم تلقیح، تلقیح با قارچPi، تلقیح با قارچ Trichو تلقیح همزمان دو قارچ)بود. نتایج تجزیه رگرسیونی بیانگر خطی بودن پاسخ بعضی از صفات از جمله قطر ساقه، تعداد برگ سبز در بوته، وزن تر و خشک شاخساره و محتوای نسبی آب برگ (RWC) نسبت به سطوح شوری بود. این صفات، با افزایش غلظت NaCl از صفر تا 240 میلی مولار، بین 13 تا 43 درصد کاهش نشان دادند. از طرفی، عکس العمل صفاتی چون ارتفاع بوته، وزن تر و خشک ساقه، و وزن تر برگ به صورت کاهشی ولی از نوع معادل های دو تکه ای بود. از سوی دیگر، پیش تیمار بذر با قارچهای همزیست به ویژه Pi و Trich+Pi رشد رویشی و محتوای آب نسبی برگ را به صورت قابل توجهی افزایش داد. همچنین نشت الکترولیت در اثر افزایش تنش شوری روند افزایشی داشت ولی پیش تیمار زیستی بذر شیب افزایش آن را نسبت به تیمار شاهد کاهش داده و از آسیب بیشتر به گیاه جلوگیری کرد. در مجموع، نتایج بیانگر نقش مثبت همزیستی بذر گندم با قارچهای P. indica و T. virens به ویژه در شرایط شور میباشد. | ||
| کلیدواژهها | ||
| اندوفیت؛ تجزیه رگرسیونی؛ نشت الکترولیت؛ قطر ساقه | ||
| عنوان مقاله [English] | ||
| The response of wheat (triticum aestivum L.) vegetative and physiological attributes to salt stress and effect of seed biopriming by Piriformospora indica and Trichoderma virens in improving salinity compatibility | ||
| نویسندگان [English] | ||
| F. Seraj1؛ N. Salimi Tamali1؛ H. Pirdashti2؛ Y. Yaghobian3 | ||
| 1M.Sc. Student/ Agronomy Department, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran. | ||
| 2Associate Prof./Agronomy Department, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran. | ||
| 3PhD. of Agronomy/Ramin Univ. of Agriculture and Natural Resources, Mollasani, Khuzestan | ||
| چکیده [English] | ||
| The present research was aimed to evaluate the response of wheat vegetative and physiological attributes to seed biopriming by Piriformospora indica (Pi) and Trichoderma virens (Trich). Experiment was conducted as factorial based on completely randomized design with four replicates. Factors were included seven levels of salt stress (0, 40, 80, 120, 160, 200 and 240 mM of NaCl) and four levels of biopriming (control, seed priming by Pi or Trich and dual inoculation by Pi+Trich). Results showed that some measured parameters such as stem diameter, green leaf number per plant, fresh and dry weights of aerial parts and relative water content (RWC) linearly respond to salt stress. These parameters were reduced from 13 to 43 % when salt stress increased from 0 to 240 mM of NaCl. By contrast, some studied parameters such as plant height, stem fresh and dry weights, leaf fresh weight reduced by increasing of salt stress as a segmented equation. Meanwhile, biopriming of Pi and Pi+Trich markedly improved vegetative parameters in addition to RWC in wheat plants. Although, salt stress increased electrolyte leakage seed biopriming could ameliorate its slope as compared to the uninoculated control and therefore prevent its damage to the plant. In conclusion, it seems that seed biopriming of wheat by growth promoting fungi, P. indica and T. virens, positively improved the growth attributes under salt stress conditions. | ||
| کلیدواژهها [English] | ||
| Endophyte, regression analysis, electrolyte leakage, relative water content | ||
| مراجع | ||
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