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Diagnosis of avian mycoplasmas: A comparison between PCR and culture technique | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده ، انتشار آنلاین از تاریخ 18 اردیبهشت 1397 | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.108217.1085 | ||
| نویسندگان | ||
Faiz Muhammad  ؛ Johur Hussain؛ Syed Khurram Fareed؛ Taseer Ahmed Khan؛ Shakeel Ahmad Khan؛ Aqeel Ahmad
| ||
| University of Karachi | ||
| چکیده | ||
| Mycoplasma gallisepticum (MG) and M. synoviae (MS) are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tool, PCR and culture is confirmatory tool for the diagnosis of mycoplasmosis after initial serological screening of suspected birds. Total 181 sample were analyzed, of this 152 (84%) and 103 (57%) samples were found positive by PCR and culture respectively. 54 (92%) samples of broiler found positive for general avian mycoplasmas. MS positivity were highest as 72 (47%) by PCR while 45 (44%) in culture among total positive samples. MG positivity was 23% and 25% in total PCR and culture positive samples. MG able to grow more frequently compared to MS from field samples. The agreement value between both tests was 67%. Overall flock wise prevalence was not much varied. The prevalence of avian mycoplasmosis was much higher than any other seasons. Study confirmed that PCR is most sensitive and reliable tool for the diagnosis of avian mycoplasmosis from field samples. | ||
| کلیدواژه ها | ||
| Avian mycoplasmosis؛ Mycoplasma gallispticum؛ Mycoplasma synoviae؛ PCR؛ Culture | ||
| عنوان مقاله [English] | ||
| تشخیص مایکوپلاسموز طیور | ||
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