Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique

Muhammad, F.; Hussain, J.; Fareed, S.  K.; Ahmad Khan, T.; Ahmad  Khan, S.; Ahmad, A.

doi:10.22092/ari.2017.108217.1085

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	Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique
Archives of Razi Institute
مقاله 9، دوره 73، شماره 3، پاییز 2018، صفحه 239-244 اصل مقاله (218.58 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22092/ari.2017.108217.1085
نویسندگان
F. Muhammad ¹^{، 2}؛ J. Hussain¹؛ S. K. Fareed¹؛ T. Ahmad Khan³؛ S. Ahmad Khan¹^{، 4}؛ A. Ahmad¹^{، 4}
¹Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan
²Department of Microbiology, Faculty of Life Sciences & Informatics Balochistan University of IT, Engineering & Management Sciences (BUITEMS), Quetta Takkatu Campus, Airport road Quetta, Balochistan, Pakistan
³Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan
⁴Department of Biosciences, Faculty of Science, Barrett Hodgson University, Karachi, Pakistan
چکیده
Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples.
کلیدواژه‌ها
Avian Mycoplasmosis؛ Mycoplasma gallispticum؛ Mycoplasma synoviae؛ PCR؛ Culture
عنوان مقاله [English]
تشخیص مایکوپلاسمای طیور: مقایسه بین روش واکنش زنجیره ای پلیمراز و روش کشت
نویسندگان [English]
فیض محمود¹^{، 2}؛ جوهر حسین¹؛ سید خرم فرید¹؛ تاثیر احمدخان³؛ شکیل احمدخان¹^{، 4}؛ عقیل احمد¹^{، 4}






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Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique