Sexual Propagation of Sequia Sempervirance (Lamb) Endl Using Mature Zygotic Embryo Culture | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 2, Volume 11, Issue 3 - Serial Number 11, March 2003, Pages 301-308 | ||
| DOI: 10.22092/ijrfpbgr.2003.116090 | ||
| Authors | ||
| A. Jafari Mofidabadi* ; S. Shahrzad | ||
| Forestry and Rangeland Research Institute | ||
| Abstract | ||
| In order to mass propagation of Sequia sempeivirance (Lamb) End I through in vitro culture, mature embryo of seed were isolated and transferred to the media. MS supplemented with 100-mg/l-casein hydrolease, 0.1 mg/l adenine-sulfate and 1 mg/l yeast extract produced highest germination of embryos (68/7%). In vitro embryo-derived shoots were used as a source for proliferation through stem cutting and adventitious shoots regeneration on a modified MS (Murashige and Skoog, 1962) agar medium supplemented with 10 mg/l 2iP, 0.5 mg/l BA and 0.1 mg/l NAA. Further multiple shoots were obtained from nodal segments taken from in vitro proliferated shoots when culture in MS medium containing 0.5 mg/l BA, 0.1 mg/l NAA and 10 mg/1 2ip. Rooting of the shoots was achieved on MS medium supplemented with 0.5 mg/l 2ip (35%). Plantlets survived in poting soil and exhibit normal growth under greenhouse conditions. | ||
| Keywords | ||
| Sequia Sempervirance; in vitro propagation; seed; Embryo | ||
| References | ||
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