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Gozari, Mohsen, Bahador, Nima, Jassbi, Amir Reza, Mortazavi, Mohammad sedigh, Eftekhar, Ebrahim. (1397). Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota. سامانه مدیریت نشریات علمی, 17(2), 413-426. doi: 10.22092/ijfs.2018.116076
Mohsen Gozari; Nima Bahador; Amir Reza Jassbi; Mohammad sedigh Mortazavi; Ebrahim Eftekhar. "Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota". سامانه مدیریت نشریات علمی, 17, 2, 1397, 413-426. doi: 10.22092/ijfs.2018.116076
Gozari, Mohsen, Bahador, Nima, Jassbi, Amir Reza, Mortazavi, Mohammad sedigh, Eftekhar, Ebrahim. (1397). 'Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota', سامانه مدیریت نشریات علمی, 17(2), pp. 413-426. doi: 10.22092/ijfs.2018.116076
Gozari, Mohsen, Bahador, Nima, Jassbi, Amir Reza, Mortazavi, Mohammad sedigh, Eftekhar, Ebrahim. Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota. سامانه مدیریت نشریات علمی, 1397; 17(2): 413-426. doi: 10.22092/ijfs.2018.116076

Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota

Iranian Journal of Fisheries Sciences
مقاله 13، دوره 17، شماره 2، تابستان 2018، صفحه 413-426  XML اصل مقاله (755.87 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.116076
نویسندگان
Mohsen Gozari1؛ Nima Bahador email 1؛ Amir Reza Jassbi2؛ Mohammad sedigh Mortazavi3؛ Ebrahim Eftekhar4
1Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Tehran, Iran.
4- Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
چکیده
Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was introducing the marine microorganisms with capability to produce antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island. Then, their intestine contents were serially diluted and treated by heating (55 °c). 100 µl of treated samples were inoculated on Starch casein nitrate agar, which is supplemented with nalidixic acid and cycloheximide. The plates were incubated at 28 °C for 4 weeks and the colonies were purified. The antioxidant activity was evaluated using DPPH radical scavenging activity and the cytotoxic activity of the isolates was screened by Brine-Shrimp micro well Cytotoxicity method. In addition, cytotoxic effect of potent crude extract was evaluated against HCT 116 and SW 480 cell lines by MTT cell proliferation assay. A new strain of marine actinobacterium was isolated with maximum antioxidant and cytotoxic activity among 17 marine actinobacterial isolates. The crude extract of the isolate scavenged DPPH radicals with IC50 value of 211.2 µg/ml. MTT cell proliferation assay revealed that the extract exhibited high toxicity against HCT 116 and SW 480 cell lines with IC50 values of 26.48 and 18.53 µg/ml respectively. The isolate identified as Streptomyces sp. strain SC 156 with 98% similarity in NCBI database. These results suggested that isolated Streptomyces sp. strain SC 156 could be considered as promising candidates for antioxidant and cytotoxic compound discovery.
کلیدواژه‌ها
Marine Streptomyces؛ cytotoxic activity؛ Antioxidant activity؛ Holothuria leucospilota
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