Somatic embryogenesis in leaf culture of Populus deltoides Bartr.ex Marshreastern | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 1, Volume 5, Issue 1 - Serial Number 5, March 2002, Pages 1-15 PDF (455.19 K) | ||
| DOI: 10.22092/ijrfpbgr.2018.115887 | ||
| Author | ||
| A. Jafari-Mofidabadi* | ||
| Department of Genetics and Plant Physiology, Research Institute of Forests anci Rangelands, P.O. Box 13185-116, Tehran, Iran | ||
| Abstract | ||
| In order to develop somatic embryogenesis for genetic manipulation process of Populus deltoides Bartrex Marsh eastern, isolated leaf disk explants were cultured on MS medium supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid), BA(benzyladenine) and glutamine. High number of somatic embryos formed on both the midveins and secondary veins. MS medium containing 2 mg/l 2,4-D and 0.1 mg/l BA with 20 J.LM glutamine showed highest number of globular-shaped embryo (11.6). Most of the embryogenic calli were formed on the cut edges of the leaf disks in MS medium containing 5 mg/12,4-D and 0.1 mg/1 BA for all glutamine concentrations. MS plant regeneration media supplemented with 2 mg/l BA and 0.1 mg/1 NAA (Naphtalene Accetic Acid) produced high percentage (72.5%) of plantlets on callused leaf disks explants. Rooted plantlets in WPM basal medium were successfully acclimatized on soil medium (1:1:1 peat, perlit and vermiculite) and transferred to the field. | ||
| Keywords | ||
| Leat disk cultute; POPULUS DELTOIDES; Somatic Embryogenesis; Emryognic calli | ||
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