Effect of Cholesterol Loaded Cyclodextrin on Vitrification of Camelus Dromedarius Sperm | ||
| علوم و فنون دامپروری | ||
| Article 6, Volume 6, Issue 23, September 2017, Pages 31-34 PDF (490.74 K) | ||
| DOI: 10.22092/aasrj.2017.115782 | ||
| Authors | ||
| Y. Nasari; F. Barati* ; Sh. Eghbal-Saeedi | ||
| Abstract | ||
| The aim of the present study was to investigate the effects of cholesterol-loaded cyclodextrin (CLC) on vitrification of one-humped Iranian camel (Camelus dormodarius). Five pairs of testis epididymides of camel were transported on ice to the laboratory and the epididymal sperm was isolated in Tris, and analyzed. Fresh sperm has been spitted to two parts; one part was exposed to CLC (1 mg/ 60×106) while the other was vitrified in a solution without CLC. The results showed that vitrification has impact on sperm parameters (P<0.05) and CLC improved sperm parameters followed by vitrification. This the first report of vitrification of camel epididymal sperm and also using CLC for improving vitrification output. | ||
| Keywords | ||
| Camelus dormadarius; Cholesterol-loaded cyclodextrin (CLC); Epididymal sperm; vitrification | ||
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