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تأثیر قارچ مایکوریزا و بسترهای مختلف کشت بر صفات کمّی و کیفی گیاه پنیرباد (Withania coagulans (Stocks) Dun.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 33، شماره 6 - شماره پیاپی 86، بهمن و اسفند 1396، صفحه 950-960 اصل مقاله (250.29 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.106765.1802 | ||
| نویسندگان | ||
| زینب محکمی* 1؛ علی میرشکار2؛ فاطمه بیدرنامنی1؛ زهرا غفاری مقدم1؛ محمد فروزنده1 | ||
| 1مربی، پژوهشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| 2استادیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| چکیده | ||
| پنیرباد (Withania coagulans (Stocks) Dun.) درختچه‌ای چند ساله از خانواده سولاناسه است. پیکره این گیاه دارای متابولیت‌های ثانویه از گروه لاکتون‌های استروئیدی با نام ویتانولوئیدها می‌باشد که امروزه اثرات فارماکولوژیکی قابل توجهی از آنها گزارش شده است. آزمایش به‌صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با فاکتور اصلی بستر کاشت در سه سطح (پرلیت + کوکوپیت، ماسه، خاک باغچه + کود دامی) و فاکتور فرعی قارچ مایکوریزا در چهار سطح (شاهد، Glomus mosseae، G. intraradice و کاربرد همزمان هر دو قارچ) در سه تکرار در پژوهشکده کشاورزی دانشگاه زابل واقع در چاه نیمه بقیه‌اله‌الاعظم (عج) اجرا شد. آنالیز فیتوشیمیایی عصاره به روش HPLC انجام شد. آنالیز آماری داده‌ها و ترسیم نمودارها به کمک نرم‌افزارهای SPSS و Excel انجام گردید. نتایج آزمایش حکایت از آن داشت که بستر خاک باغچه + کود دامی و کاربرد همزمان هر دو قارچ بهترین اثر را بر افزایش صفات طول ساقه، وزن تر و وزن خشک داشت. در بررسی اثر ساده بستر بر میزان متابولیت‌های ثانویه مشاهده شد که تیمار خاک باغچه + کود دامی باعث افزایش متابولیت‌های ثانویه ویتافرین آ، ویتانولید آ، گلوکوویتانولید، ویتااسترامونولید، ویتانوزید X و کوآگولین در این آزمایش شد. کاربرد همزمان هر دو قارچ نیز باعث افزایش بیشتر متابولیت‌های ثانویه (بجز ویتااسترامونولید و کوآگولین) گردید. نتایج حکایت از آن داشت که کاربرد همزمان دو گونه قارچ G. mosseae و G. intraradice در بستر خاک باغچه + کود دامی سبب افزایش صفات مورفولوژیکی و ویتانولوئیدها در گیاه دارویی پنیرباد شد. | ||
| کلیدواژه‌ها | ||
| بستر کشت؛ لاکتون‌های استروئیدی؛ مایکوریزا؛ ویتانولوئیدها؛ Withania coagulens (Stocks) Dun | ||
| عنوان مقاله [English] | ||
| Effect of mycorrhiza fungi and different substrates on quantitative and qualitative characteristics of Withania coagolense (Stocks) Dun. | ||
| نویسندگان [English] | ||
| Z. Mohkami1؛ A. Mirshekar2؛ F. Bidarnamani1؛ Z. Ghaffari Moghadam1؛ M. Forouzandeh1 | ||
| 1Agricultural Research Institute, University of Zabol, Zabol, Iran | ||
| 2Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| چکیده [English] | ||
| Withania coagolense (Stocks) Dun. is a perennial shrub, belonging to the Solanaceae family. The study species is rich in a group of valuable compounds as steroidal lactones called withanolides. Nowadays, significant pharmacological properties of withanolides have been reported. The effects of mycorrhizal fungi symbiosis and substratewere studied on withania in the greenhouse of Agricultural Institute at the University of Zabol. The study was conducted in a factorial experiment based on a completely randomized design. The main plot was substrate (cocopit + perlit, garden soil+ manure, sand) and the subplot consisted of mycorrhizal fungi (control, Glomusintraradice, G. mosseae,simultaneous use of both fungi). Phytochemical analysis of the extract was performed by HPLC. Statistical analysis was done using Excel and SPSS software. The results indicated that the garden soil+ manure and simultaneous use of both fungi had the best effect on increased shoot length and fresh and dry weight. According to the results, the garden soil+ manure resulted in increased production of secondary metabolites. The simultaneous use of both fungi resulted in higher production of secondary metabolites (except withastramonolid and coagolin). Our results clearly showed that the simultaneous use of both fungi in garden soil+ manure resulted in increased morphological traits and withastramonolid in the study specie. | ||
| کلیدواژه‌ها [English] | ||
| Substrate, steroid lactones, mycorrhizal fungi, Withania coagolense (Stocks) Dun, withanolides | ||
| مراجع | ||
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