Rapid method with high efficiency to transform alfalfa for expression of BANYULS (BAN) gene | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 7, Volume 13, Issue 3 - Serial Number 21, October 2006, Pages 295-338 PDF (927.24 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2005.115163 | ||
| Authors | ||
| ُُSeyed Mohsen Hesamzadeh Hejazi* 1; S. Abdemishani2; S. Arcioni3 | ||
| 1Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran. | ||
| 2Tehran University | ||
| 3Genetic and Biotechnology Research Institiue of Forage crops, Italy | ||
| Abstract | ||
| Condensed tannins (CTS) are flavonoid oligomers, many of which have beneficial effects on animal (safe bloat) and human health. The functions of CT are due to their ability to form reversible complex with proteins, the formation of these protein-tannin complexes making protein unavailable for microbial enzyme and activate other mechanisms like deprivation of metal ions and inhibition of oxidative phosphorylation which also results in the prevention of foam formation and bloating. Anthocyanins are flavonoids that contribute to the red, violet or blue pigmentation of both seeds and flowers. The BAN gene encodes anthocyanidin reductase (ANR), an enzyme proposed to convert anthocyanidins to their corresponding 2, 3-cis -flavan -3-ols ((+)-catechin). Transformation of leaf explants from Medicago sativa var. Rgsy27 and M.sativa var. P1 genotypes was carried out with the plasmid pBI121.BAN(binary vector containing the BAN gene) by two strains of Agrobacterium tumefaciens(LBA 4404 & EHA 105). Leaf explants of Rgsy27 produced more than 90% proembryogenic calli, but in P1 produced about 30% .More than 65% of regenerated embryos were changed to whole plants in both genotypes. The efficiency of transformation in Rgsy27 and P1 plants was depended on Agrobacterium strains, so that the strain LBA 4404 was efficient more than EHA 105 for transformation of both genotypes. Ectopic expression of the BAN in Alfalfa transgenic foliage resulted in accumulation of CTs. Thus, it has been assumed that the BAN gene also acts as starter units in tannins condensation in alfalfa. Loss of the BAN gene function increases the amount of red pigment, suggesting that the BANYULS protein might be a negative regulator of pigment production. BANYULS activity is limiting factor for CT production, making this enzyme an attractive target for induction of over expression in forage plants, such as alfalfa (Medicago sativa) with the goal of inducing CT production in their foliage. The condensed tannin percentage in dry matter of transgenic Rgsy27 and P1 plants was 0.474 and 0.600, respectively and 0.259 and 0.408 in control plants. | ||
| Keywords | ||
| Alfalfa; Agrobacterium tumefaciens; Arabidopsis thaliana; BAN gene; Proanthocyaninidins(CT); Regeneration and Transformation | ||
| References | ||
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