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شیوع گسترده تیپ A ویروس زردی نکروتیک رگبرگ چغندرقند در ایران | ||
| چغندرقند | ||
| مقاله 4، دوره 33، شماره 2، دی 1396، صفحه 163-176 اصل مقاله (298.23 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/jsb.2018.107517.1128 | ||
| نویسندگان | ||
| مریم ابراهیم قمی1؛ سید باقر محمودی2؛ فرشاد رخشنده رو* 3؛ مسعود نادرپور4؛ پیمان نوروزی5 | ||
| 1دانشجوی دکتری گروه بیماری شناسی گیاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2دانشیار موسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات آموزش و ترویج کشاورزی ، کرج، ایران | ||
| 3استادیار گروه بیماری شناسی گیاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 4استادیار پژوهش، موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| 5دانشیار موسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| هدف از این تحقیق، بررسی ژنوتیپهای مختلف Beet necrotic yellow vein (BNYVV) در ایستگاههای تحقیقاتی چغندرقند کشور شامل طرق در خراسان رضوی، زرقان در فارس، میاندوآب در آذربایجان غربی و اکباتان در همدان بود. همزمان نمونههایی از مزارع حوزه کارخانههای قند تربتجام در خراسان رضوی، نهاوند در استان همدان، کمالآباد در استان البرز، نقده در استان آذربایجانغربی و قزوین در استان قزوین و نیز مزارع کشاورزان اراک در استان مرکزی مطالعه شد. جمعا 58 نمونه آلوده ریشه چغندرقند از این ایستگاهها و مزارع فوق مورد بررسی قرار گرفتند. همچنین تعداد 30 نمونه خاک آلوده از مزارع چغندرقند مغان، طرق، زرقان و کمالآباد کرج جمع آوری و جهت تعیین نوع تیپ ویروس عامل بیماری رقم حساس در آنها کشت شد. برای تعیین نوع تیپهای A و B، ناحیه TGB ژنوم ویروس BNYVV با روش multiplex PCR تکثیر شد. به علاوه، با تکثیر قسمتی از ژنوم RNA5 ویروس از طریق تکنیک مولکولی RT-PCR و جفت آغازگر اختصاصی، ردیابی تیپ P یا J انجام گردید. بر این اساس در بین نمونههای آلوده مورد بررسی با روش multiplex PCR و جفت آغازگرهای اختصاصی A و B تنها قطعه 324 جفت بازی تکثیر گردید. به این ترتیب در بین 88 نمونه آلوده مورد بررسی، بدون استثنا تیپ A شناسایی و هیچکدام از تیپهای B، P و Jردیابی نشدند. جدایههای BNYVV حاوی RNA5 در هیچیک از نمونه ها یافت نشد. | ||
| کلیدواژه‌ها | ||
| چغندرقند؛ Multiplex PCR؛ تیپ A؛ RNA5؛ BNYVV | ||
| عنوان مقاله [English] | ||
| Extensive outbreak of the type A of Beet Necrotic Yellow Vein Virus in Iran | ||
| نویسندگان [English] | ||
| M. Ebrahim-Ghomi1؛ B. Mahmoodi2؛ F. akhshanderoo3؛ M. Naderpour4؛ P. Norouzi5 | ||
| 1PHD Student of Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Associate professor of Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 3Assistant professor of Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 4Assistant professor of Seed and Plant Certification and Registration Institute(SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 5Associate professor of Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| The present study aimed to evaluate different Beet necrotic yellow vein virus (BNYVV) genotypes in sugar beet research stations in Iran including Toroq, Zarqan, Miandoab and Ekbatan. Some samples were also collected from sugar factories’ field in Torbat-e Jam, Nahavand, Kamalabad, Naqadeh and Qazvin and also sugar beet fields in Arak. A total of 58 infected sugar beet root samples were collected. In addition, 30 soil samples were collected from sugar beet fields in Moghan, Toroq, Zarqan and Kamalabad, and a susceptible cultivar was planted in them to determine the causal agent type. To detect type A or B of the virus, TGB region of the virus genome was multiplied using multiplex-PCR. Moreover, partial multiplication of RNA5 using RT-PCR and specific primers was performed to detect type P or J. Only 324 bp fragment was multiplied using multiplex-PCR and specific primers designed for type A or B. Therefore, among 88 samples, only type A was detected. No BNYVV isolates containing RNA5 was observed in any of the samples. | ||
| کلیدواژه‌ها [English] | ||
| BNYVV, Multiplex PCR, Rhizomania, RNA5, sugar beet, Type A | ||
| مراجع | ||
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