Study of callus induction and regeneration in Populus euphratica Oliv. via ovary culture | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 6, Volume 15, Issue 3 - Serial Number 29, November 2007, Pages 231-242 PDF (352.88 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2007.114951 | ||
| Authors | ||
| S. Sadat* 1; M.H. Assareh1; A. Jafari Mofid Abadi2; S.R. Tabaei -Aghdaei3; A. Ghamari Zare3 | ||
| 1Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran | ||
| 2Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran. | ||
| 3- Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran | ||
| Abstract | ||
| Immature and mature ovaries of Populus euphratica Oliv.were harvested from the female trees on the bank of Karun river. Five sterilization treatments were tested in a completely randomized design (CRD) with eight replications. 0.1% mercuric chloride and 70% ethanol both for 30 seconds, 70% ethanol for 30 seconds and 25% sodium hypochlorite for 10 minutes and also 70% ethanol for 1 minute and 25% sodium hypochlorite for 10 minutes were the best treatments. In order to Callus induction, four treatments were evaluated through a covariance analysis in a CRD design with six replications. MS basal medium supplemented with 3 mgl-1 IBA and 0.1 mgl-1 BAP and also 3 mgl-1 2,4-D and 0.1 mgl-1 Kin were proposed for callus induction. In addition, the regression equation between the explants age and the amount of callus production was obtained. Regression curve demonstrated the decrease of callus induction as a result of increase in explants age. Regeneration was studied using four hormonal treatments in a factorial experiment with four replications based on a completely randomized block design. MS basal medium supplemented with 2 mgl-1 BAP and 0.5 mgl-1 NAA was suggested as the best treatment. Also, combination of NAA and IBA both 0.1 mgl-1 in half MS medium was suggested as the best treatments for root production. After the different stages of acclimatization in soil, the plantlets were transferred to field. | ||
| Keywords | ||
| Ovaries; MS medium; Callus; regeneration; rooting and acclimatization | ||
| References | ||
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