Study of tissue culture in dioecious sorrel | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 3, Volume 15, Issue 4, February 2008, Pages 273-283 PDF (436.01 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2008.114936 | ||
| Authors | ||
| A.M. Shakib* 1; M. Ahmad-Raji2; M. Orujloo2; A. Izadi-Darbandi3 | ||
| 1Agricultural Biotechnology Research Institute, Iran. | ||
| 2Agricultural Biotechnology Research Institute | ||
| 3College of Agriculture, Shahed University. | ||
| Abstract | ||
| Sorrel(Rumex acetosa L.) is a perennial species, being used as a model plant in molecular genetic studies of sex determination, because of several characteristics such as dioecy, sex chromosomes and short life cycle it is In this study regeneration potentials of different tissues such as cotyledon, hypocotyl, leaf, petiole and flowering stem cultured on media containing different cytokinin and auxins combinations are presented. The results showed callus induction in cultured tissues on media containing auxins. Tissues cultured on media containing cytokinins alone or in combinations with auxins showed regeneration response. The highest regeneration rate 100% was obtained from stem segments cultured on medium containing 1 mg/l TDZ. Leaf and petiole after stem had regeneration rate of 35% and 40 % on medium containing 1 mg/l TDZ and 0.75 mg/l IAA and NAA. The highest number of regenerants per explant was 8.8 using stem segments cultured on medium containing TDZ. Histological observations showed that regenerated shoots originated from the cells in the area of the vascular tissues. Regenerated shoots rooted on medium without hormone and plants produced seeds after growth in glasshouse conditions. | ||
| Keywords | ||
| Sorrel (Rumex acetosa L); explant; callus induction and regeneration tissue culture | ||
| References | ||
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Ainsworth, C., Crossley, S., Buchanan-Wollaston, V., Tangavelu, M. and Parker, J., 1995. Male and female flowers of the dioecious plant sorrel show different patterns of MADS box gene expression. The Plant Cell 7: 1583-1598. Culafic, L., Budimir, S., Vujicic, R. and Nelskovic, M., 1987a. Induction of somatic embryogenesis and embryo development in Rumex acetosella L. Plant Cell, Tissue and Organ Culture, 11: 133-139. Culafic, L., Samofalova, A. and Nesieovic, M., 1987b. In Vitro organogenesis in two dioecious species, Rumex acetosella L. and R. acetosa L. (Polygonaceae). Plant Cell, Tissue and Organ Culture, 11: 125-131. Parker, J.S. and Clark, M.S., 1991. Dosage sex chromosom system in plants. Plant Science, 80, 79-92. Shakib, A.M., 1999. MADS-box genes in sorrel (Rumex acetosa L.). Ph.D Thesis, University of London. | ||
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