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Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. (1393). Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing. سامانه مدیریت نشریات علمی, 13(2), 384-393. doi: 10.22092/ijfs.2018.114364
M Mirbakhsh; A Akhavan sepahy; M Afsharnasab; A Khanafari; M.R Razavi. "Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing". سامانه مدیریت نشریات علمی, 13, 2, 1393, 384-393. doi: 10.22092/ijfs.2018.114364
Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. (1393). 'Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing', سامانه مدیریت نشریات علمی, 13(2), pp. 384-393. doi: 10.22092/ijfs.2018.114364
Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing. سامانه مدیریت نشریات علمی, 1393; 13(2): 384-393. doi: 10.22092/ijfs.2018.114364

Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing

Iranian Journal of Fisheries Sciences
مقاله 11، دوره 13، شماره 2، تیر 2014، صفحه 384-393
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114364
نویسندگان
M Mirbakhsh* ؛ A Akhavan sepahy؛ M Afsharnasab؛ A Khanafari؛ M.R Razavi
چکیده
A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).
کلیدواژه‌ها
Vibriosis؛ shrimp diseases؛ Litopenaeus vannamei؛ 16S rDNA sequencing
آمار
تعداد مشاهده مقاله: 308


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