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Alinezhad, S., Tolouee, M., Kamalzadeh, A., Motalebi, A. A., Nazeri, M., Yasemi, M., Shams-Ghahfarokhi, M., Tolouei, R., Razzaghi-Abyaneh, M.. (1390). Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi. سامانه مدیریت نشریات علمی, 10(3), 363-374. doi: 10.22092/ijfs.2018.114144
S. Alinezhad; M. Tolouee; A. Kamalzadeh; A. A. Motalebi; M. Nazeri; M. Yasemi; M. Shams-Ghahfarokhi; R. Tolouei; M. Razzaghi-Abyaneh. "Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi". سامانه مدیریت نشریات علمی, 10, 3, 1390, 363-374. doi: 10.22092/ijfs.2018.114144
Alinezhad, S., Tolouee, M., Kamalzadeh, A., Motalebi, A. A., Nazeri, M., Yasemi, M., Shams-Ghahfarokhi, M., Tolouei, R., Razzaghi-Abyaneh, M.. (1390). 'Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi', سامانه مدیریت نشریات علمی, 10(3), pp. 363-374. doi: 10.22092/ijfs.2018.114144
Alinezhad, S., Tolouee, M., Kamalzadeh, A., Motalebi, A. A., Nazeri, M., Yasemi, M., Shams-Ghahfarokhi, M., Tolouei, R., Razzaghi-Abyaneh, M.. Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi. سامانه مدیریت نشریات علمی, 1390; 10(3): 363-374. doi: 10.22092/ijfs.2018.114144

Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi

Iranian Journal of Fisheries Sciences
مقاله 1، دوره 10، شماره 3، مهر 2011، صفحه 363-374
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114144
نویسندگان
S. Alinezhad؛ M. Tolouee؛ A. Kamalzadeh؛ A. A. Motalebi؛ M. Nazeri؛ M. Yasemi؛ M. Shams-Ghahfarokhi؛ R. Tolouei؛ M. Razzaghi-Abyaneh*
چکیده
  In the present study, mycobiota and natural occurrence of aflatoxin B1 (AFB1) in pellet feed and feed ingredients used in a feed manufacturing plant for rainbow trout nutrition was investigated. The samples were cultured on the standard isolation media for 2 weeks at 28 ºC. Identification of fungal isolates was implemented based on the macro- and microscopic morphological criteria. AFB1 was detected using high performance liquid chromatography (HPLC). Based on the results obtained, a total of 109 fungal isolates were identified of which Aspergillus was the prominent genus (57.0%), followed by Penicillium (12.84%), Absidia (11.01%) and Pseudallscheria (10.10%). The most frequent Aspergillus species was A. flavus (60.66%) isolated from all feed ingredients as well as pellet feed. Among 37 A. flavus isolates, 19 (51.35%) were able to produce AFB1 on YES broth in the range of 10.2 to 612.8 µg/g fungal dry weight. HPLC analysis of trout feed showed that pellet feed and all feed ingredients tested except gluten were contaminated with different levels of AFB1 in the range of 1.83 to 67.35 µg/kg. Unacceptable levels of AFB1 were reported for feed including soybean, fish meal and wheat. These results indicate the importance of AF contamination of trout feed in amounts higher than the acceptable level as a risk factor for fish farming production.
کلیدواژه‌ها
aflatoxin؛ Oncorhinchus mykiss؛ Aspergillus flavus؛ mycobiota؛ Troutfeed
آمار
تعداد مشاهده مقاله: 314


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