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بررسی فیتوشیمیایی عصاره گیاه Loganberry (Rubus×Loganobaccus) تکثیر شده از طریق کشت بافت | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 3، دوره 33، شماره 5 - شماره پیاپی 85، دی 1396، صفحه 717-729 اصل مقاله (340.83 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2017.109508.1960 | ||
| نویسندگان | ||
| شیما مریخ1؛ محبوبه طاهرخانی* 2؛ سید رضا حسینی دوست3 | ||
| 1دانشجوی کارشناسی ارشد، گروه آموزشی فیتوشیمی و شیمی فناوری اسانس، دانشکده شیمی دارویی، واحد علوم دارویی، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2استادیار، گروه شیمی، دانشکده علوم پایه، واحد تاکستان، دانشگاه آزاد اسلامی، تاکستان، ایران | ||
| 3استاد، گروه میکروبیولوژی، دانشکده علوم و فناوریهای نوین، واحد علوم دارویی، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| گیاه مورد مطالعه، گیاهی هیبریدی با نام علمی Loganberry (Rubus×Loganobaccus) از خانواده Rosaceae و جنس Rubusمیباشد که میوهای مانند توتفرنگی میدهد. میوه این گیاه قرمز و بسیار ترش است و این گیاه بسیار کمیاب، یکی از ارقام تمشک قرمز میباشد. Loganberry بومی ایران نبوده و در حال حاضر از طریق کشت درون شیشهای تکثیر میگردد. تاکنون تحقیقی از نظر فیتوشیمیایی بر روی گونه هیبریدی Loganberry انجام نشده است. در این تحقیق، عصاره برگ گیاه Loganberry Rubus×Loganobaccus)، تکثیر شده از طریق کشت درون شیشهای، از نظر ترکیبهای طبیعی مورد بررسی قرار گرفت. برای انجام این تحقیق، ابتدا مراحل عصارهگیری و چربیگیری انجام شد. عصاره روی ستون کروماتوگرافی برده شد و از نظر ترکیبهای تشکیلدهنده بررسی شد. سپس ترکیب طبیعی با استفاده از روشهای مختلف طیفسنجی IR، 1H-NMR، 13C-NMR و dept 135 شناسایی شد. نتایج حاصل از روشهای طیفسنجی منجر به شناسایی یک فلاونوئید به نام هسپریدین (Hesperidin) شد. | ||
| کلیدواژهها | ||
| Loganberry؛ کشت درون شیشه ای؛ عصاره؛ هسپریدین | ||
| عنوان مقاله [English] | ||
| Phytochemical investigation on the extract of loganberry, propagated via plant tissue culture | ||
| نویسندگان [English] | ||
| Sh. Merikh1؛ M. Taherkhani2؛ S.R. Hosseini Doust3 | ||
| 1M.Sc. student, Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Chemistry, College of Science, Takestan Branch, Islamic Azad University, Takestan, Iran | ||
| 3Department of Microbiology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| The loganberry (Rubus × loganobaccus), belonging to the Rosaceae family and Rubus genus, gives fruits like strawberry. The fruit is red and very sour and this rare species is one of the cultivars of red raspberry. Loganberry is not native to Iran and currently is propagated through in vitro plant tissue culture. So far, phytochemical research has not been conducted on loganberry. In present study, the leaf extract of loganberry, propagated through in vitro culture, was investigated phytochemically. The extract of loganberry was purified and the obtained natural product was elucidated by NMR spectroscopy techniques. In this study, a flavonoid, namely hesperetin, was isolated from the leaves of the loganberry. This structure was elucidated by spectral methods (1H-NMR, 13C-NMR, IR and dept 135). | ||
| کلیدواژهها [English] | ||
| Loganberry, plant tissue culture, extraction, hesperidin | ||
| مراجع | ||
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