| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,251 |
| تعداد مقالات | 35,918 |
| تعداد مشاهده مقاله | 54,585,143 |
| تعداد دریافت فایل اصل مقاله | 93,700,322 |
In vitro Response of Asparagus breslerianus to NaCl | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 6، دوره 6، شماره 2، آذر 2017، صفحه 153-163 اصل مقاله (405.84 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2017.113538 | ||
| نویسندگان | ||
| Seyyed Javad Mousavizadeh* ؛ Mohammad Reza Hassandokht؛ Abdolkarim Kashi | ||
| Department of Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. | ||
| چکیده | ||
| Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to 0, 21.88, 43.76, 65.64, 76.58, 87.52, 109.40, 131.28, 153.16 and 175.04 mM NaCl in MS (Murashige and Skoog) basal medium supplemented with 0.88 µM BA (6-Benzylaminopurine) and 1.07 µM NAA (1- Naphthalene acetic acid). According to results, friable and compact type, green and white green calli were obtained up to 87.52 mM NaCl. Soft type, yellow and cream calli were observed with increasing salinity further 109.4 mM NaCl. Calli growth and plantlets regeneration were high in media up to 109.4 mM NaCl. In term of mineral accumulation, sodium content increased with an increase in NaCl levels. It indicates that A. breslerianus calli could acclimatize to salt stress by high osmotic adjustment. These suggested that, under in vitro salt-induced osmotic stress, A. breslerianus is reflected as salt resistant which tolerate NaCl up to 109.4 mM. | ||
| کلیدواژه‌ها | ||
| Asparagus؛ Salt tolerance؛ Callus type؛ Callus growth | ||
| مراجع | ||
|
1. Shannon MC, Grieve CM. Tolerance of vegetable crops to salinity. Sci Hortic. 1999;78:5-38.
2. Akhani H, Ghorbanli M. A contribution to the halophyte vegetation and flora of Iran. Towards the rational use of high salinity tolerant plants. 1993;1:35-44.
3. Francois LE. Salinity effects on asparagus yield and vegetative growth. J Am Soc Hortic Sci. 1987;112:432-436.
4. Kruistum GV, Poll JT, Meijer J, Lievens M. Effect of NaCl on Asparagus quality, production and mineral leaching. Acta Hort. 2008;776:87-90.
5. Bajji M, Lutts S, Kinet JM. Physiological changes after exposure to and recovery from polyethylene glycol-induced water deficit in callus cultures issued from durum wheat (Triticum durum desf.) cultivars differing in drought resistance. J Plant Physiol. 2000;156:75–83.
6. Venkataiah P, Christopher T, Subhash K.Selection and characterization of sodium chloride and mannitol tolerant callus lines of red pepper (Capsicum annuum L.).Plant Physiol. 2004;9:158-163.
7. Mills D. Differential response of various tissues of Asparagus officinalis to sodium chloride. J Exp Bot. 1989;40:485-491.
8. Bekheet SA, Taha HS, Sawires ES, El-Bahr MK. Salt stress in tissue cultures of Asparagus officinalis. Egyp J Hort. 2000;27:275-187. (Abstract).
9. Stajner N, Bohanec B, Jakse M. In vitro propagation of Asparagus maritimus - A rare Mediterranean salt-resistant species. Plant Cell Tiss Organ Cult. 2002;70:269-274.
10. Rechineer Kh. Flora Iranica. Liliaceae. 1982;15:1-31.
11. Yuritsyna NA. Vegetation of saline habitats on southeastern border of europe. Arid Eco. 2012;2:239-244.
12. Mousavizadeh SJ, Hassandokht MR, Kashi A. Multivariate analysis of edible Asparagus species in Iran by morphological characters. Euphytica. 2015;206:445-457.
13. Mousavizadeh SJ, Hassandokht MR, Kashi A, Gil J, Cabrera A, and Moreno R.Physical mapping of 5S and 45S rDNA genes and ploidy levels of Iranian Asparagus species. Sci Hortic. 2016;211:269-276.
14. Castro P, Gil J, Cabrera A, Moreno R. Assessment of genetic diversity and phylogenetic relationships in Asparagus species related to Asparagus officinalis. Genet Resour Crop Ev. 2013;60:1275-1288.
15. Moreno R, Espejo JA, Cabrera A, Millan T, Gil J. Ploidic and molecular analysis of ‘Morado de Huetor’ asparagus (Asparagus officinalis L.) population; a Spanish tetraploid landrace, Gen. Res. Crop Evol. 2006;53:729-736.
16. Regalado JJ, Carmona-Martin E, Castro P, Moreno R, Gil J, Encina CL. Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs. Plant Cell Tiss Organ Cult. 2015;121:501-510.
17. Sheidai M, Inamdar AC. Polyploidy in the genus Asparagus L, Nucleus. Calcutta. 1992;35:93-97.
18. Sun YL, Hong SK. Effects of plant growth regulators and L-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.). Plant Cell Tiss Organ Cult. 2010;100:317-328.
19. Ghahreman A. Flora of Iran in natural color. Research Institute of Forests and Rangelands, Tehran. 1997; Number 2389. Cod 148.
20. Arnon DI. Photo synthesis by isolated chloroplast. Biochem Biphys. 1956;20:440-461.
21. Shah SH, Wainwright SJ, Merrett MJ. The interaction of sodium and calcium chlorides and light on growth, potassium nutrition, and proline accumulation in callus cultures of Medicago sativa L. New Phytol. 1990;116:37-45.
22. Hamada AM, EL-enany AE. Effect of NaCl salinity on growth, pigment and mineral element contents, and gas exchange of broad bean and pea plants. Biol Plant. 1994;36:75-81.
23. Ikeuchi M, Sugimoto K, Iwase A. Plant Callus: Mechanisms of Induction and Repression. The Plant Cell. 2013;25:3159-3173.
24. Naidoo G, Somaru R, Achar P. Morphological and physiological responses of the halophyte, Odyssea paucinervis (Staph) (Poaceae), to salinity. Flora. 2008;203:437-447.
25. Sharma V, Ramawat KG. Salinity-induced modulation of growth and antioxidant activity in the callus cultures of miswak (Salvadora persica). 3 Biotech. 2013;3:11-17.
26. Ahmad MSA, Javed F, Javed S, Alvi AKh. Relationship between callus growth and mineral nutrients uptake in salt-stressed indica rice callus. J Plant Nutri. 2009;32:382-394.
27. Aazami MA, Torabi M, Shekari F. Response of some tomato cultivars to sodium chloride stress under in vitro culture condition. Afri J Agri Res. 2010;5:2589-2592.
28. Yuxia Z, Wei-wei T, Yan-shu W, Jian-hui W. Effect of alkali-salt stress on anti-oxidative enzymes of Asparagus officinalis. Journal of Inner Mongolia University for Nationalities (Natural Sciences). 2006;21:165-168.
29. Errabii T, Gandonou ChB, Essalmani H, Abrini J, Idaomar M, Senhaji NS. Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiol Plant. 2007;29:95-102.
30. Benderradji L, Brini F, Kellou K, Ykhlef N, Djekoun A, Masmoudi Kh, Bouzerzour H. Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions. Agronomy. 2012;367851:1-8.
31. Zinnah KMA, Zobayer N, Sikdar SU, Liza LN, Chowdhury MN, Ashrafuzzaman M. In vitro Regeneration and Screening for Salt Tolerance in Rice (Oryza sativa L.). Int Res J Biol Sci. 2013;2:29-36.
32. Carmona-Martin E, Regalado JJ, Raghavan R, Encina CL. In vitro induction of autooctoploid asparagus genotypes. Plant Cell Tiss Organ Cult. 2014;121:249-254.
33. Lokhande VH, Nikam TD, Penna S. Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L. Plant Cell Tiss Organ Cult. 2010;102:17-25.
34. Tester M, Davenport R. Na+ tolerance and Na+ transport in higher plants. Ann Bot. 2003;91:503-527.
35. Madava-Rao KV, Raghavendra AS, Janardhan-Reddy K. Physiology and Molecular Biology of Stress Tolerance in Plants. Springer. 2006;345.
36. Melgar JC, Syvertsen JP, Martinez V, Garcia-sanchez F. Leaf gas exchange, water relations, nutrient content and growth in citrus and olive seedlings under salinity. Biol Plant. 2008;52:385-390.
37. Zekri M. Effect of NaCl on growth and physiology of sour orange and Cleopatra mandarin seedlings. Sci Hortic. 1991;47:305-315.
38. Flowers TJ, Troke PF, Yeo AR. The mechanisms of salt tolerance in halophytes. Annu Rev Plant Physiol. 1977;28:89-121.
39. Woodward AJ, Bennett IJ. The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tiss Organ Cult. 2005;82:189-200. | ||
|
آمار تعداد مشاهده مقاله: 737 تعداد دریافت فایل اصل مقاله: 679 |
||