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تأثیر قارچ Funneliformis mosseae در بیان ژن دهیدرین در نهالهای پسته ایرانی تحت تنش خشکی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 11، دوره 25، شماره 2 - شماره پیاپی 50، مهر 1396، صفحه 314-323 اصل مقاله (218.12 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.113319 | ||
| نویسندگان | ||
| توران فیضی کمره1؛ رامین رحمانی* 2؛ حسن سلطانلو3؛ محمد متینی زاده4 | ||
| 1دانشجوی دکترای جنگلشناسی و اکولوژی جنگل، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 2عضو هیات علمی گروه جنگلشناسی و اکولوژی جنگل دانشکده علوم جنگل دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 3دانشیار، گروه بیوتکنولوژی و اصلاح نباتات، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 4دانشیار پژوهشی، بخش تحقیقات جنگل، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران | ||
| چکیده | ||
| قارچهای میکوریزی آربوسکولار یکی از مهمترین و فراوانترین میکروارگانیسمهای خاکزی هستند. این قارچها با 80 تا 90 درصد از گونههای گیاهی همزیستی برقرار میکنند. همزیستی میکوریزی میتواند الگوی بیان ژن دهیدرین را تحت تنش خشکی تغییر دهد. پروتئینهای دهیدرین، در زمان تنش خشکی باعث آبگیری سلولهای گیاهی میشوند، در سلول تجمع کرده، از ساختار غشاها و ماکرومولکولها حفاظت میکنند. هدف از انجام این پژوهش، ارزیابی عملکرد قارچ Funneliformis mosseae در تغییر بیان ژن دهیدرین نهالهای میکوریزی پسته ایرانی در طول دوره تنش خشکی است. همه نهالها بهمدت دو سال، سه بار در هفته آبیاری شدند، سپس تنش خشکی بهمدت نه روز اعمال شد که در این مرحله علائم پژمردگی در برگ نهالها مشاهده شد، سپس نهالها آبیاری دوباره شدند. نتایج نشان داد که تنش خشکی منجر به کاهش درصد کلونیزاسیون قارچ در ریشه نهالهای پسته ایرانی شد. بیان ژن دهیدرین در نهالهای پسته ایرانی (بدون میکوریز) در نهمین روز اعمال تنش خشکی بهطور معنیداری نسبت به شاهد افزایش یافت، اما همزیستی پسته ایرانی با قارچF.mosseaeبیان ژن دهیدرین را تحت تنش خشکی، بهطور معنیداری کاهش داد. در نهایت میتوان گفت که افزایش بیان ژن دهیدرین راهکار استفاده شده توسط همزیستی میکوریزی برای مقاومت به خشکی نهالهای پسته ایرانی نبوده است، شاید مهمترین عملکرد قارچهای میکوریزی در تحمل به خشکی گیاهان، از طریق اجتناب از تنش خشکی در گیاهان است. | ||
| کلیدواژهها | ||
| تنش خشکی؛ پسته ایرانی؛ دهیدرین؛ کلونیزاسیون؛ نهال میکوریزی | ||
| عنوان مقاله [English] | ||
| Evaluating the effects of Funneliformis mosseae on expression of dehydrin during drought stress on Iranian pistachio (Pistacia vera L.) | ||
| نویسندگان [English] | ||
| T. Feizi Kamareh1؛ Ramin Rahmani2؛ H. Soltanloo3؛ M. Matinizade4 | ||
| 1Ph.D. student, College of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran. | ||
| 2Associate Professor Department of Silviculture and Forest Ecology Faculty of Forest Sciences Gorgan University of Agricultural Sciences and Natural Resources (GUASNR) | ||
| 3Assoc. Prof., College of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran | ||
| 4Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran | ||
| چکیده [English] | ||
| Arbuscular mycorrhizal fungi are one of the most important and the most abundant micro-organisms in soil. The fungi have symbiotic relation with 80 to 90 percent of plant species. Arbuscular mycorrhizal symbiosis is able to alter pattern of dehydrin expression under drought stress. During period of drought acclimation that causes dehydration of cells, dehydrin proteins accumulate inside cells and protect membranes and macromolecules structures. All seedlings were irrigated three times per week during two years then were exposed to severe drought stress for nine days until wilt symptoms appeared on their leaves, then irrigated normally. It was evaluated whether Funneliformis mosseae is able to alter expression functions of dehydrin in the mycorrhiza seedlings of Pistacia vera under drought stress. Results showed that mycorrhizal colonization percentage in roots of P. vera declined significantly due to water stress treatments. The result revealed that the expression of dehydrin was increased significantly in non-mycorrhizal seedlings at 9 days exposure to the drought stress compared to the mycorrhizal seedlings, but the levels of dehydrin accumulation in mycorrhizal treatment subjected to drought were considerably lower than that of corresponding non-mycorrhiza seedlings, indicating that the accumulation of dehydrin proteins is not a mechanism by which the mycorrhizal symbiosis protects their host seedlings and suggesting that mycorrhizal seedlings were less strained by drought due to primary drought-avoidance mechanisms | ||
| کلیدواژهها [English] | ||
| colonization, dehydrin, Drought Stress, Iranian pistachio, mycorrizal seedling | ||
| مراجع | ||
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