| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,262 |
| تعداد مقالات | 36,058 |
| تعداد مشاهده مقاله | 55,183,982 |
| تعداد دریافت فایل اصل مقاله | 94,183,493 |
ارزیابی تنوع ژنتیکی جمعیت‌های بادرنجبویه (Melissa officinalis L.) از نظر صفات زراعی، بازده اسانس و غلظت سیترال | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 8، دوره 25، شماره 2 - شماره پیاپی 50، مهر 1396، صفحه 271-288 اصل مقاله (329.97 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.113316 | ||
| نویسندگان | ||
| محمد حسن رحیمی* 1؛ سعید اهری‌زاد2؛ ناصر محب‌علی‌پور3 | ||
| 1دانش آموخته دکتری تخصصی اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهرکرد | ||
| 2استاد، گروه بهنژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز | ||
| 3استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی، واحد میانه، میانه | ||
| چکیده | ||
| بادرنجبویه به‌عنوان یک گیاه دارویی ارزشمند در درمان و تسکین بیماریها کاربرد فراوانی داشته و شناخت کافی از تنوع ژنتیکی و طبقهبندی آن به‌منظور انتخاب ژنوتیپهای مناسب برای اهداف بهنژادی، ضروری بهنظر می‌رسد. از این‌رو، این تحقیق به‌منظور بررسی تنوع ژنتیکی و روابط بین صفات در 11 جمعیت بادرنجبویه شامل نُه جمعیت ایرانی و دو جمعیت خارجی با استفاده از طرح بلوک‌های کامل تصادفی در سه تکرار طی سا‌ل‌های 1390 و 1391 در مزرعه تحقیقاتی دانشگاه تبریز انجام شد. نتایج حاصل از تجزیه واریانس، بیانگر وجود تنوع ژنتیکی معنی‌دار از نظر اغلب صفات مورد مطالعه بود. بررسی پارامترهای ژنتیکی نشان داد که صفات درصد و عملکرد اسانس، تعداد ساقه در بوته و وزن تر ساقه و غلظت سیترال در بوته بالاترین مقادیر مربوط به پیشرفت ژنتیکی، ضرایب تنوع ژنتیکی و فنوتیپی را داشتند و بیشترین میزان وراثت‌پذیری عمومی بهوزن تر ساقه در بوته (71/0=2h)، درصد اسانس (65/0=2h) و تعداد ساقه در بوته (58/0=2h) مشاهده شد. جمعیت اردبیل از نظر غلظت سیترال، درصد و عملکرد اسانس در زمره جمعیت‌های برتر قرار داشت. تجزیه همبستگی نشانگر رابطه مثبت و معنی‌دار عملکرد اسانس با شاخص کلروفیل، طول و عرض برگ و درصد اسانس و همچنین همبستگی منفی و معنی‌دار با تعداد گره بود. در تجزیه به عامل‌ها بر مبنای مقادیر ویژه بزرگ‌تر از یک بهروش تجزیه به مؤلفه‌های اصلی، پنج عامل تعیین شد که در مجموع 40/87 درصد از تغییرات کل را تبیین کردند. تجزیه خوشه‌ای جمعیت‌های مورد مطالعه را در سه گروه قرار داد. با توجه به تنوع مشاهده شده در این پژوهش، امکان انتخاب جمعیت‌های مطلوب و استفاده از آنها در برنامه‌های بهنژادی مرتبط با بهبود صفات زراعی و اسانس وجود دارد. | ||
| کلیدواژه‌ها | ||
| اسانس؛ بادرنجبویه؛ تنوع ژنتیکی؛ صفات زراعی | ||
| عنوان مقاله [English] | ||
| Evaluation of genetic diversity in populations of lemon balm (Melissa officinalis L.) in terms of agronomic traits, essential oil and citral concentration | ||
| نویسندگان [English] | ||
| M.H. Rahimi1؛ S. Aharizad2؛ N. Mohebalipour3 | ||
| 1PhD, Graduated from Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Shahrekord, I.R. Iran | ||
| 2Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tabriz, I.R. Iran. | ||
| 3Assist. Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Islamic Azad University, Mianeh Branch, I.R. Iran | ||
| چکیده [English] | ||
| With many applications in treating and relieving of illnesses, lemon balm (Melissa officinalis L.) is considered as a valuable medicinal plant; as a result, sufficient knowledge about its genetic variation and its classification is necessary for selecting suitable genotypes for breeding purposes. Therefore, present study was to investigate genetic diversity and relationships between several traits of 11 populations of lemon balm consisted of nine Iranian plant populations and two foreign plant populations using randomized completely block design with three replications during 2011 and 2012 in research farm of University of Tabriz. Results of analysis of variance showed that genetic diversity for most of the traits was significant. Study of genetic parameters revealed that percentage and yield of essential oil, number of stems per plant, fresh weight of stem and citral concentration had the highest amounts of genetic advance, genetic and phenotypic coefficient of variations. The highest heritability was observed on fresh weight of stems per plant (h2=0.71), essential oil percentage (h2=0.59) and number of stem per plant (h2=0.58). Ardabil population was superior in terms of citral concentration and percentage and yield of essential oil among the populations. Pearson correlation coefficients indicated a positive and significant relationship between essential oil yield with chlorophyll index, leaf length and width and essential oil percentage and also a significant negative correlation with number of nodes. In factor analysis based on principal component analysis method with eigenvalues greater than one, five factors were determined that a total of 87.40% of the total variance was explained by them. Cluster analysis divided the studied populations into three groups. Due to the observed variability in the present study, selection of desirable populations for breeding programs regarding to improvement of agronomic traits and essential oil of the species is possible. | ||
| کلیدواژه‌ها [English] | ||
| agronomic traits, essential oil, Genetic diversity, lemon balm | ||
| مراجع | ||
|
- Abbaszadeh, B., Farahani, H.A., Valadabadi, S.A. and Darvishi, H.H., 2009. Nitrogenous fertilizer influence on quantity and quality values of balm (Melissa officinalis L.). Journal of Agricultural Extension and Rural Development, 1(1): 031-033. - Abdoshahi, R., 2014. Introduction to multivariate statistics. Shahid Bahonar University of Kerman Press, Iran, 240 pp (In Persian). - Adzet, T., Ponz, R., Wolf, E. and Schulte, E., 1992. Genetic variability of the essential oil content of Melissa officinalis. Planta Medicine, 58:558-561. - Aharizad, S., Rahimi, M.H., Moghadam, M. and Mohebalipour, N., 2012. Study of genetic diversity in lemon balm (Melissa officinalis L.) populations based on morphological traits and essential oils content. Annals Biological Res, 3(12): 5748-53. - Ahmadian Yazdeli, M., Tallei, D., Kordnaeech, A. and Forghani, A.R., 2016. Genetic structure of lemon balm (Melissa officinalis L.) based on morphological analysis. Proceedings of the Twenty-Fourth National Congress of Genetics. Shahid Beheshti University, Tehran (In Persian). - Allard, R.W., 1999. Principles of Plant Breeding. John Wiley & Sons, New York. - Askari, F. and Sefidkon, F., 2004. Essential oil composition of Melissa officinalis L. from different regions. Iranian Journal of Medicinal and Aromatic Plants Research, 20(2): 229-237 (In Persian). - Aziz, E.E. and El-Ashry, S.M., 2009. Efficiency of slow release urea fertilizer on yield and essential oil production of lemon balm (Melissa officinalis L.) plant. American-Eurasian Journal of Agricultural and Environmental Science, 5(2): 141-147. - Babalar, M., Khoshsokhan, F., Fattahi Moghadam, M.R. and Pourmeidani, A., 2013. Assessment of morphological diversity and oil yields in some populations of thyme (Thymus kotschyanus Boiss. & Hohen). Iranian Journal of Horticultural Science, 44 (2): 119-128 (In Persian). - Bigdelou, M., Nazeri, V. and Hadian, J., 2013. Evaluation of morphological characters in different populations Kermani thyme (Thymus caramanicus Jalas) species native to Iran. Journal of Crop and Farming Breeding Plants, 1(2): 145-160 (In Persian). - Bisset, N.G. and Wichtl, M., 1994. Herbal drugs. Medpharm Scientific Publishers, Germany, 352 pp. - Brezovac, N., Carović, K., Kolak, I., Britvec, M. and Šatović, Z., 2005. Characterization and evaluation of lemon balm (Melissa officinalis) accessions held at Croatian bank of plant genes. Sjemenarstvo, 23(1): 57-66. - Chahal, G.S. and Gosal S.S., 2002. Principles and procedures of plant breeding: biotechnological and conventional approaches. CRC Press Boca Raton, England, 604 pp. - Cosge, B., Ipek, A. and Gurbuz, B., 2009. GC/MS analysis of herbage essential oil from lemon balms (Melissa officinalis L.) grown in Turkey. Journal of Applied Biological Sciences, 3(2): 136-139. - Eyvazi, A. and Heydari Rikan, M., 2011. Medicinal plants (with an emphasis on improving them). Jahad Daneshgahi Urmia University Press, Iran, 184 pp (In Persian). - Falconer, D.S., 1960. Introduction to Quantitative Genetics. Pearson Education, India, 305 pp. - Farshadfar, A., 2010. Principles and Multivariate Statistical Methods. Taq-e Bostan Press, Iran, 754 pp (In Persian). - Fufa, H., Baenziger, P.S., Beecher, B.S., Dweikat, I., Graybosch, R.A. and Eskridge, K.M., 2005. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 145: 133-146. - Heidari, S., Azizi, M., Soltani, F. and Hadian, J., 2014. Foliar application of Ca (NO3)2 and KNO3 affects growth, essential oil content, and oil composition of French tarragon. Industrial Crops and Products, 62: 526-532. - Immanuel, S.C., Pothiraj, N., Thiyagarajan, K., Bharathi, M. and Rabindran, R., 2011. Genetic parameters of variability, correlation and path coefficient studies for grain yield and other yield attributes among rice blast disease resistant genotypes of rice (Oryza Sativa L.). African Journal of Biotechnology 10: 3322-3334. - Jafarzadeh, A.A., kasrayi, R. and Neyshabouri, M.R., 1997. Detailed studies of 18 hectares of land and soils research station Karkaj. Journal of Agricultural Knowledge, 7 (1): 187-213 (In Persian). - Keyvanpajouh, S., 2011. Genetic diversity of Iranian yarrow (Achillea ssp.) populations using molecular markers and assessment of their essential oils. M.Sc thesis, University of Guilan, Iran, 105 pp (In Persian). - Kheradmannia, M. and Alirezaei, M., 2015. An introduction to multivariate statistics. Negarkhaneh Press, Iran. 222 pp (In Persian). - Khorsand, A., 2012. Medicinal plants. Shahrab Press, Iran, 212 pp (In Persian). - Madani, Z. and Fotukian, M.H., 2014. Genetic Diversity of some morphological traits in four populations of lemon balm Melissa officinalis L. 1th Electronic Conference on Environment and Findings in Agricultural Ecosystems. University of Tehran, Iran (In Persian). - Makeen, K., Abrahim, G., Jan, A. and Singh, A.K., 2007. Genetic variability and correlation studies on yield and its components in mungbean [Vigna radiata (L.) Wilczek]. Journal of Agronomy, 3: 25-34. - Mohammadi, S.A. and Prasanna, B.M., 2003. Analysis of genetic diversity in crop plants—salient statistical tools and considerations. Crop Science, 43(4): 1235-1248. - Moradkhani, H., Sargsyan, E., Bibak, H., Naseri, B., Sadat-Hosseini, M., Fayazi-Barjin, A. and Meftahizade, H., 2010. Melissa officinalis L., a valuable medicine plant: Journal of Medicinal Plants Research, 4: 2753-2759. - Muhammed, A., 2012. Genetic diversity in plants (Vol. 8). Springer Science and Business Media, USA, 506 pp. - Muller, S.F. and Klement, S., 2006. A combination of valerian and lemon balm is effective in the treatment of restlessness and dyssomnia in children. Phytomedicine, 13: 383-387. - Omidbeigi, R., 2005. Production and processing of medicinal plants. Astan Quds Razavi Press, Iran, 347 pp (In Persian). - Osman Sari, A. and Ceylan, A., 2002. Yield characteristics and essential oil composition of lemon balm (Melissa officinalis L.) grown in the Aegean region of Turkey. Turkish Journal of Agriculture and Forestry, 26: 217-224. - Patora, J., Majda, T., Góra, J. and Klimek, B., 2003. Variability in the content and composition of essential oil from lemon balm (Melissa officinalis L.) cultivated in Poland. Acta Poloniae Pharmaceutica, 60: 395-400. - Perry, E.K., Pickering, A.T., Wang, W.W., Houghton, P.J. and Perry, N.S., 1999. Medicinal plants and alzheimer’s disease: from etnobotany to phytotherapy. Journal of Pharmacy, 51: 527-534. - Saglam, C., Atakisi, I., Turhan, H., Kaba, S., Arslanoglu, F. and Onemli, F., 2004. Effect of propagation method, plant density and age of lemon balm (Melissa officinalis L.) herb and oil yield. New Zealand Journal of Crop and Horticultural Scienc, 32 (4): 419-423. - Salamati, M. and Yousefi, M., 2014. Evaluation of variation for yield and morphological traits in Dracocephalum moldavica L. genotypes. Journal of Plant Research, 27(1): 91-99 (In Persian). - Seidler-Łożykowska, K., Bocianowski, J. and Król, D., 2013. The evaluation of the variability of morphological and chemical traits of the selected lemon balm (Melissa officinalis L.) genotypes. Industrial Crops and Products, 49: 515-520. - Sen, S., Chakraborty, R. and De, B., 2011. Challenges and opportunities in the advancement of herbal medicine: India’s position and role in a global context. Journal of Herbal Medicine, 1(3): 67-75. - Sorensen, J.M., 2000. Melissa officinalis, essential oil, authenaticity, production and pharmalogical activity. International Journal of Aromatherapy, 10: 7-15. - Talle, B., Darvish, F., Mohammadi, A., Abbaszadeh, B. and Rohami, M., 2012. Assessment of relationship between effective traits on yield and compounds of essential oil and morphological traits of lemon balm (Melissa officinalis L.) accessions using path analysis and canonical correlation. Journal of Basic and Applied Scientific Research, 2: 3719-3723. - Tomlinson, T.R. and Akerele, O., 2015. Medicinal plants: their role in health and biodiversity. University of Pennsylvania Press, USA, 221 pp. - Toth, J., Mrlianova, M., Tekelova, D. and Koremova, M., 2003. Rosmarinic acid an important phenolic active compound of lemon balm (Melissa officinalis L.). Acta Facultatis Pharmaceuticae Universitatis Comenianae, 50: 146-139. | ||
|
آمار تعداد مشاهده مقاله: 1,312 تعداد دریافت فایل اصل مقاله: 808 |
||