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تأثیر پوترسین و تیدیازورون بر اندامزایی درون شیشهای گیاه مریم‌گلی (Salvia officinalis L.) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 3، دوره 25، شماره 2 - شماره پیاپی 50، مهر 1396، صفحه 201-211 اصل مقاله (538.59 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.113311 | ||
| نویسندگان | ||
| سمیرا جعفری1؛ محمدحسین دانشور2؛ محمدرضا صالحی سلمی* 3؛ امین لطفی جلال آبادی4 | ||
| 1دانش‌آموخته کارشناسی ارشد گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان | ||
| 4استادیار گروه زراعت، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| چکیده | ||
| مریم‌گلی (Salvia officinalis L.) یکی از با ارزش‌ترین گیاهان تیره نعناع است و بومی ایران، مدیترانه، جنوب‌شرق آفریقا، آسیای مرکزی و جنوب آمریکا گسترش است. با توجه به قوه نامیه پایین بذر و پرهزینه و وقتگیر بودن تکثیر رویشی آن از یکسو و از سوی دیگر حفظ ذخایر ژرمپلاسم این گیاه دارویی در معرض انقراض، تکثیر انبوه آن توسط تکنیکهای کشت بافت امری ضروریست. در این مطالعه اندامزایی غیرمستقیم مریم‌گلی مورد بررسی قرار گرفت. ریزنمونههای برگ (5/0 در 5/0 سانتیمتر) و میانگره (1 سانتیمتر)، از گیاهچههای 45 روزه رشد کرده در شرایط درون شیشهای، در محیط کشت موراشیگ و اسکوگ (MS) حاوی 12 ترکیب مختلف از 2,4-D یا NAA بههمراه BAP قرار گرفتند. بهترین کالوسزایی از ریزنمونههای میانگره در محیط کشت MS حاوی 1 میلیگرم در لیتر NAA و 5/0 میلیگرم در لیتر BAP بهدست آمد. بهترین شاخهزایی (66/5 عدد) از کالوسهای بهدست آمده از ریزنمونههای میانگره در محیط کشت MS حاوی 50 میلیگرم در لیتر پوترسین و 5/0 میلیگرم در لیتر تیدیازورون بهدست آمد. بهترین رشد طولی شاخه و ریشهزایی در محیط کشت MS حاوی 1 میلیگرم در لیتر IBA ایجاد شد و گیاهان حاصل در گلخانه سازگار شدند. این روش میتواند بهمنظور باززایی گیاهان پس از انتقال ژن و بدون تغییر شیمری، مورداستفاده قرار گیرد. | ||
| کلیدواژه‌ها | ||
| پلی‌آمین؛ دارویی؛ ریشه‌دهی؛ شاخهزایی؛ کالوس؛ کشت بافت | ||
| عنوان مقاله [English] | ||
| Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis L. | ||
| نویسندگان [English] | ||
| S. Jafari1؛ M.H. Daneshvar2؛ M.R. Salehi Salmi3؛ Amin Lotfi Jalal-Abadi4 | ||
| 1M.Sc., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran. | ||
| 2Prof., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran | ||
| 3Assist. Prof., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran. | ||
| 4epartment of Agronomy, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran | ||
| چکیده [English] | ||
| In this study, an indirect organogenesis regeneration protocol for Salvia officinalis L. is described. Leaf (0.5 × 0.5 cm) and internode (1 cm in length) explants sections, from 45 day old in vitro germinated plant, were cultured in Murashige and Skoog (MS) basal medium supplemented with 12 different combinations of 2,4-dichlorophenoxy acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The best calli induction response was observed when 1 mg/l NAA and 0.5 mg/l BAP combination was applied to the internode explants. Also, results showed an interesting possibility for the stimulation of shoot regeneration (5.66) through internode segments cultured on MS medium containing 50 mg/l putrescine and 0.5 mg/l TDZ. Shoot elongation and rooting were achieved on MS medium with 1mg/l IBA. Excellent acclimatization to greenhouse conditions was observed for all transferred plantlets. By this procedure no morphological differences were observed between the regenerated and mother plants. This protocol may be also utilized to carry out plant regeneration after genetic transformation, in order to develop transformed plants without the presence of chimeric zones. | ||
| کلیدواژه‌ها [English] | ||
| Callus, Medicinal, Polyamine, rooting, Shoot regeneration, Tissue culture | ||
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