| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,272 |
| تعداد مقالات | 36,149 |
| تعداد مشاهده مقاله | 55,713,859 |
| تعداد دریافت فایل اصل مقاله | 94,492,362 |
A Molecular and Serological Study on Visceral Leishmaniasis in Asymptomatic Stray Dogs in Mashhad, Iran | ||
| Archives of Razi Institute | ||
| مقاله 3، دوره 72، شماره 4، اسفند 2017، صفحه 231-235 اصل مقاله (112.5 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.113300 | ||
| نویسندگان | ||
| S. Sabzavari1؛ G. Razmi* 1؛ A. Naghibi1؛ J. Khoshnegah2 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Zoonotic visceral leishmaniasis is caused by Leishmania infantum (L. infantum),and its major reservoir hosts are domestic dogs, most of which are asymptomatic. This study aimed to detect L. infantum spp. in asymptomatic stray dogs by molecular and serological methods in Mashhad, Iran, during 2011-12. In this study, 94 asymptomatic stray dogs were randomly selected and their blood samples were collected for indirect fluorescent antibody testing. Furthermore, tissue samples from all the L. infantum seropositive stray dogs were examined using semi-nested polymerase chain reaction (PCR). Accordding to the results, 11.7 %(11/94) of the dogs were L. infantum seropositive. The PCR positivity rate of L. infantum was 63.6% (7/11) in at least one of the collected specimens of the seropositive dogs. The L. infantum kinetoplast DNA (kDNA) was detected in the liver of 36% (4/11), the spleen of 27% (3/11), and the skin of 54.5 %(6/11) of the stray dogs. In this study, based on the molecular and serological examinations, visceral leishmaniasis infection among the stray dogs in Mashhad was confirmed. | ||
| کلیدواژه‌ها | ||
| Leishmania Infantum؛ Stray dogs؛ Mashhad | ||
| عنوان مقاله [English] | ||
| بررسی سرولوژی و ملکولی لیشمانیوز احشایی در سگهای ولگرد فاقد علائم در شهر مشهد | ||
| نویسندگان [English] | ||
| صدف سبزواری1؛ غلامرضا رزمی1؛ ابوالقاسم نقیبی1؛ جواد خوش نگاه2 | ||
| 1گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 2گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| چکیده [English] | ||
| بیماری لیشمانیوز احشایی ناشی از لیشمانیا انفانتوم بوده و سگهای اهلی به عنوان مخزن بیماری شناسایی شده اند. بسیاری از سگهای آلوده فاقد علائم بالینی بیماری بوده و به عنوان مخزن بیماری شناخته میشوند . هدف این مطالعه بررسی سرولوژی و ملکولی لیشمانیوز احشایی در سگهای ولگرد شهرستان مشهد میباشد. از سال 1390 تا 1392، تعداد 94 قلاده سگ بطور اتفاقی انتخاب و نمونههای خون برای آزمایش ایمونوفلورسنت غیر مستقیم جمع آوری شدند. علاوه بر این نمونههای بافتی جمع آوری شده سگهای ولگرد سرم مثبت جمع آوری شده با روش seminested-PCR بررسی ملکولی شدند. در این بررسی نمونههای سرمی 11 قلاده سگ ( 7/11%)، واجد عیار سرمی مثبت بر علیه لیشمانیا اینفانتوم بودند. همچنین آلودگی به لیشمانیا اینفانتوم با روش seminested-PCR در 7 قلاده از سگهای ولگرد سرم مثبت (6/63% ) تایید گردید. وجود kDNAلیشمانیا اینفانتوم به ترتیب در 36% بافت کبد، 27% بافت طحال و 5/54% بافت پوست سگهای ولگرد سرم مثبت تعیین شدند. در این مطالعه بر پایه نتایج سرولوژی و ملکولی وجود آلودگی به لیشمانیا اینفانتوم دربین سگهای ولگرد فاقد علائم شهر مشهد تعیین گردید. | ||
| کلیدواژه‌ها [English] | ||
| لیشمانیا اینفانتوم, سگ ولگرد, شهر مشهد | ||
| مراجع | ||
|
Adinezadeh, A., Eshrat Beigom, K., Mohebali, M., Shojaee, S., Rokni, M.B., Zarei, Z., et al., 2013. Endoparasites of stray dogs in Mashhad, Khorasan Razavi province, Northeast Iran with special reference to zoonotic parasites.Iran J Parasitol 8, 459. Aransay, A.M., Scoulica, E., Tselentis, Y., 2000. Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66, 1933-1938. Azizi, K., Rassi, Y., Javadian, E., Motazedian, M., Rafizadeh, S., Yaghoobi Ershadi, M., et al., 2006. Phlebotomus (Paraphlebotomus) alexandri: a probable vector of Leishmania infantum in Iran. Ann Trop Med Parasitol 100, 63-68. Baneth, G., Day, M., Roura, X., Shaw, S, 2005. Leishmaniosis. In : Shaw S; Day M (Eds.). Arthropod-borne infectious diseases of the dog and cat pp:89-99. Bokai, S., Mobedi, I., Edrissian GhH, N.A., 1998. Seroepidemiological study of canine visceral leishmaniasis in Meshkin-Shahr, northwest of Iran. Arch Inst Razi 41, 48-49. Camargo, J., Langoni, H., Troncarelli, M., Machado, J., Lucheis, S.B., Padovani, C., 2010. Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis. J Venom Anim Toxins Incl Trop Dis 16, 414-420. Dantas-Torres, F., 2007. The role of dogs as reservoirs of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Vet Parasitol 149, 139-146. de Andrade, H.M., Reis, A.B., dos Santos, S.L., Volpini, Â.C., Marques, M.J., Romanha, A.J., 2006. Use of PCR–RFLP to identify Leishmania species in naturally-infected dogs. Vet Parasitol 140, 231-238. de Queiroz, N., da Silveira, R., de Noronha, A., Oliveira, T., Machado, R., Starke-Buzetti, W., 2011. Detection of Leishmania (L.) chagasi in canine skin. Vet Parasitol 178, 1-8. Haddadzade, H., Fattahi, R., Mohebali, M., Akhoundi, B., Ebrahimzade, E., 2013. Seroepidemiologcal investigation of visceral leishmaniasis in stray and owned dogs in Alborz province, Central Iran using direct agglutination test. Iran J Parasitol 8, 152. Hamarsheh, O., Nasereddin, A., Damaj, S., Sawalha, S., Al-Jawabreh, H., Azmi, K., et al., 2012. Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine. Hamidi, A., Nadim, A., Edrissian, G.H., Tahvildar-Bidruni, G., Javadian, E., 1982. Visceral leishmaniasis of jackals and dogs in northern Iran. Trans R Soc Trop Med Hyg 76, 756-757. Heidarpour, M., Pourtaghi, M., Khoshnegah, J., 2014. Prevalence and risk factors for canine leishmaniasis in Mashhad, north east of Iran. Iran J Vet Sci Tech 4, 37-46. Hosseininejad, M., Mohebali, M., Hosseini, F., Karimi, S., Sharifzad, S., Akhoundi, B., 2012. Seroprevalence of canine visceral leishmaniasis in asymptomatic dogs in Iran. Iran J Vet Res 13, 54-57. Kirmse, P., Mahin, L., Lahrech, T., 1987. Canine leishmaniasis in Morocco with special reference to infantile kala-azar. Trans R Soc Trop Med Hyg 81, 212-213. Lachaud, L., Marchergui-Hammami, S., Chabbert, E., Dereure, J., Dedet, J.P., Bastien, P., 2002. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol 40, 210-215. Mahshid, M., Baharak, A., Iraj, S., Sina, K., Javad, K., Mehdi, B., 2014. Seroprevalence of canine visceral leishmaniasis in southeast of Iran. J Parasit Dis 38, 218-222. Maia, C., Ramada, J., Cristóvão, J.M., Gonçalves, L., Campino, L., 2009. Diagnosis of canine leishmaniasis: conventional and molecular techniques using different tissues. The Veterinary Journal 179, 142-144. Mancianti, F., Gradoni, L., Gramiccia, M., Pieri, S., Marconcini, A., 1986. Canine leishmaniasis in the Isle of Elba, Italy. Trop. Med. Parasitol 37, 110-112. Manna, L., Vitale, F., Reale, S., Caracappa, S., Pavone, L.M., Della Morte, R., et al., 2004. Comparison of different tissue sampling for PCR-based diagnosis and follow-up of canine visceral leishmaniosis. Vet Parasitol 125, 251-262. Mohebali, M., Hajjaran, H., Hamzavi, Y., Mobedi, I., Arshi, S., Zarei, Z., et al., 2005. Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet Parasitol 129, 243-251. Mohebali, M., Hamzavi, Y., Fallah, E., Zarei, Z., 2001. Study of canine visceral leishmaniasis in some parts of Iran and its health importance. Tehran Univ Vet Fac J 56, 55-59. Moshfe, A., Mohebali, M., Edrissian, G., Zarei, Z., Akhoundi, B., Kazemi, B., et al., 2009. Canine visceral leishmaniasis: asymptomatic infected dogs as a source of L. infantum infection. Acta Trop 112, 101-105. Nadim, A., Navid-Hamidid, A., Javadian, E., Bidruni, G.T., Amini, H., 1978. Present status of kala-azar in Iran. Am J Trop Med Hyg 27, 25-28. Rassi, Y., Azizi, K., Motazedian, M., Javadian, E., Rafizadeh, S., Fakhar, M., et al., 2007. The seminested PCR based detection of Leishmania infantum infection in asymptomatic dogs in a new endemic focus of visceral leishmaniasis in Iran. Iran J Arthropod Borne Dis 1, 38-42. Reis, L.E.S., Coura-Vital, W., Roatt, B.M., Bouillet, L.É.M., Ker, H.G., de Brito, R.C.F., et al., 2013. Molecular diagnosis of canine visceral leishmaniasis: a comparative study of three methods using skin and spleen from dogs with natural Leishmania infantum infection. Vet Parasitol 197, 498-503. Sabzevari, S., Razmi, G.R., Naghibi, A., Khoshnegah, J., 2013. A serological study of Leishmania infantum in dogs of Khorasan Razavi province, Iran. J Parasit Dis 37, 189-191. Solano-Gallego, L., Koutinas, A., Miró, G., Cardoso, L., Pennisi, M., Ferrer, L., et al., 2009. Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis. Vet Parasitol 165, 1-18. Xavier, S.C., de Andrade, H.M., Monte, S.J.H., Chiarelli, I.M., Lima, W.G., Michalick, M.S.M., et al., 2006. Comparison of paraffin-embedded skin biopsies from different anatomical regions as sampling methods for detection of Leishmania infection in dogs using histological, immunohistochemical and PCR methods. BMC Vet Res 2, 17. | ||
|
آمار تعداد مشاهده مقاله: 13,295 تعداد دریافت فایل اصل مقاله: 62,468 |
||