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Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Milk Thistle (Silybum marianum L. Gaertn.) under Tissue Culture Medium | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 8، دوره 6، شماره 1، تیر 2017، صفحه 59-69 اصل مقاله (312.68 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2017.113151 | ||
| نویسندگان | ||
| Sahar Eari؛ Mahnaz Aghdasi* ؛ Elham Ahmadzadeh؛ Manijeh Mianabadi | ||
| Faculty of Science, Departement of Biology, Golestaan University, Gorgan, Iran | ||
| چکیده | ||
| The Silybum marianum (L.) Gaertn. is the dicotyledonous herbs of the Asteraceae family that is important in medical industry. The biological active compound of S. marianum is a mixture of several flavonolignals generally known as silymarin. The purpose of this study was to optimize S. marianum tissue culture for callus induction, silymarin production and comparison of some biochemical traits between explants and its derived callus. In this experimental study, the seeds were surface sterilized and transferred to MS medium to achieve sterile seedlings. Then, the effects of different concentrations of 2,4-D either alone or in combination with Kin were investigated on callus induction and silymarin production of root and leaf explants from sterile seedlings. The experiment was performed in complete randomized design with three replicates. The results revealed that the highest percentage of callus induction and silymarin accumulation (14.4%) were observed with 0.5 and 1 mg/L 2,4-D and Kin in root explant after one month. The quantitative and qualitative data from HPLC method revealed that the major flavonolignans in the root and its derived callus was isosilybin B (ISBNB). But the main component in leaf explant and its derived callus was silydianin (SDN). The results showed that silymarin level in root and leaf explants was lower than their derived callus. Meanwhile lignin amount in the root and leaf explants was much higher than corresponding callus. In addition, peroxidase activity was significantly higher in callus derived from root explant, compared to leaf explant and its derived callus. The current data demonstrated that callus derived from root explants can be an efficient source for silymarin production. | ||
| کلیدواژه‌ها | ||
| Antioxidant؛ Callus؛ Flavonolignan؛ silymarin | ||
| مراجع | ||
|
1. Nikolova MT, Ivancheva SV. Quantitative flavonoid variations of Artemisia vulgaris L. and Veronica chamaedrys L. in relation toaltitude and polluted environment. Acta Biol Szeged. 2005;49:29-32.
2. Cacho M, Moran M, Corchete P, Frarandez-Tarrago J. Influence of medium composition on the accumulation of flavonolignans in cultured cells of Silybum marianum (L) Gaertn. Plant Sci. 1999;144:63-68.
3. Kurkin VA, Zapeschnaya GG, Volotsueva AV, Avdeeva EV, Pimenovm KS. Flavolignans of Silybum marianum fruit. Chem Nat Prod. 2001;37:315-317.
4. Flora K, Hahn M, Banner K, Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol. 1998;93:139-143.
5. Kren V, Walterova D. Silybin and silymarin-New effects and application. Biomed Pap. 2005;149:29-41.
6. Kropácová K, Misúrová E, Haková H. Protective and therapeutic effect of silymarin on the development of latent liver damage. Radiats Biol Radioecol. 1998;38:411-415.
7. Santosh k. Silymarin and skin cancer prevention: Anti-inflammatory, antioxidant and immunomodulatory effects. Int J Encol 2004;18:101-112.
8. Sanchez-Sampedro MA, Fernandez-Tarrago J. Enhanced silymarin accumulation is related to calcium deprivation in cell suspension cultures of Silybum marianum. Plant Physiol. 2005;162:1-6.
9. Kordi H, Aghdasi M, Khalafi M. An investigation on flavonolignans in different organs of Silybum marianum L. in Gorgan region. Iranian J Med Aroma Plants. 2013;3:651-665.
10. Rahnama H, Hasanloo T, Shams MR, Sepehrifar R. Silymarin production by hairy root culture of Silybum marianum (L.) Gaertn. Iranian J Biotech. 2008;6:113-118.
11. Elhaak M, Zayed M, Mattar M, Gad G, Dietz K. Optimization of Silybum marianum L. callus production and magnifying callus silymarin accumulation by elicitors or precursors. Int J Adv Pharm Biol Chem. 2016;5:148-161
12. Asghari G, Saidfar GA, Mahmudi S. Biotransformation of Aromatic Aldehydes by Cell Cultures of Peganum harmala L. and Silybum marianum (L.) Gaertn. Iranian J Pharm Res. 2004;3:127-130.
13. HasanlooT, Khavari-Nejad RA, Majidi E, Shams Ardakani MR. Flavonolignan Production in Cell Suspension Culture of Silybum marianum. Pharmacol Biol. 2008;12:876-882.
14. Rady MR, Matter MA, Ghareeb HA, Hanafy MS, Saker MM, Eid SA, Hammoda FM, Imbaby SI, Nazief NH. In vitro cultures of Silybum marianum and silymarin accumulation. J Genet Engend Biotechnol. 2014;12:75-79.
15. Cimino C, Cavalli SV, Spina F, Natalucci C, Priolo N. Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases. Elect J Biotechnol. 2006;9:1-4.
16. Khalili M, Hasanloo T, KazemiTabar SK, Rahnama H. Influence of exogenous salicylic acid on flavonolignans and lipoxygenase activity in the hairy root cultures of Silybum marianum. Cell Biol Int. 2009;33:988-94.
17. Alikaridis F, Papadakis D, Pantelia K, Kephalas T. Flavonolignan production from Silybum marianum transformed and untransformed root cultures. Fitotrapia. 2000;71:379-384.
18. Murashing T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 1962;15:473-497.
19. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and pralin contetents in Burkina Fasan honey, as well as their scavenging activity. Food Chem. 2005;91:571-577.
20. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10:178-182.
21. Iiyama K, Wallis AFA. Determination of lignin in herbaceous plants by an improved acetyl bromide procedure. J Sci Food Agric. 1990;51:145-161.
22. Sykłowska-Baranek k, Pietrosiuk A, Naliwajski MR, Kawiak A, Jeziorek M, Wyderska S, Lojkowska E, Chinou I. Effect of L-phenylalanine on PAL activity and production of naphthoquinone pigments in suspension cultures of Arnebiaeuchroma (Royle) Johnst. In Vitro Cell Develop Biol. 2012;48:555-564.
23. Kar M, Mishra D. Catalase, peroxidase, and Polyphenoloxidase activities during Rice leave senescence. J Plant Physiol. 1976;57:315-319.
24. Chance B, Maehly AC. Assay of catalase and peroxidases. Methods Enzymol. 1995;11:755-764.
25. Hobbie LJ. Auxins, Molecular genetic approach in Arabidopsis. Plant Physiol Biochem. 1998;36:91-102.
26. Mok MC. Cytokinins and plant development–An overview. In Cytokinins: Chemistry, Activity, and Function, ed. DWS Mok, MC Mok. Boca Raton, Florida: CRC Press. 1994;155-66.
27. Skoog F, Miller CO. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957;11:118-130.
28. El sheriff F, KhattabS, Ibrahim A. Improved silymarin content in elicited multiple shoot cultures of Silybum marianum L. Physiol Mol Biol Plants. 2013;19:127-136.
29. Saba M, Iqbal PS, Srivastav A. In Vitro Micropropagation of Silybum marianum L. from Various Explants and Silybin Content. J Plant Biochem Biotechnol. 2000;9:81-87.30. Heller W, Forkmann G. Biosynthesis. In: Harbone JB (ed) the flavonoids. London, UK: Chapman and Hall. 1988.
31. Schmid JPW, Doemer SD, Clouse RA, Dixon CJ, Lam B. Developmental and environmental regulation of a bean chalkone synthase promoter in transgenic tobacco. Plant Cell. 1990;2:619-631.
32. Freudenberg K, Neish AC. Constitution and Biosynthesis of Lignins. New York, USA: Springer Verlag. 1968. | ||
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