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Effect of Silybum marianum methanolic extract on nutritional indices, crustacean cardioactive peptide, α-amylase and protease activities of Helicoverpa armigera (Lep.: Noctuidae) | ||
| Journal of Entomological Society of Iran | ||
| مقاله 2، دوره 37، شماره 2 - شماره پیاپی 69، آبان 2017، صفحه 169-180 اصل مقاله (1.06 M) | ||
| نوع مقاله: Paper, English | ||
| نویسندگان | ||
| Vahid Mohammadi Gisour؛ Azam Mikani* ؛ Saeid Moharramipour | ||
| Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| چکیده | ||
| The efficacy of milk thistle, Silybum marianum methanolic extract was investigated on the third instar larvae of cotton bollworm, Helicoverpa armigera (Hübner). The experiments were done at 25±1 °C, 65±5 RH and photoperiod of 16:8 (L: D) h. The LC50 and LC20 values were estimated 10449 and 5654 ppm, respectively. One hundred microliters of the plant extract at 5654 ppm was added to 0.9 grs of artificial diet. Nutritional indices, crustacean cardioactive peptide (CCAP) content and digestive enzymatic (α-amylase and protease) activities were measured after 72 h. Plant extract decreased nutritional indices including approximate digestibility (AD), relative growth rate (RGR), relative consumption rate (RCR), efficiency of conversion of ingested food (ECI) and efficiency of digested food (ECD). Methanolic extract of S. marianum decreased α-amylase and protease activities in the midgut of H. armigera. The activity of α-amylase from 194 mU in control decreased to 86.8 mU in treatment. It also decreased protease activity from 108.2 mU in control to 60.6 mU in treatment. Incubation of dissected midgut with CCAP increased α-amylase and protease activities in H. armigera whereas the buffer alone had no effect. Feeding on artificial diet containing methanolic extract of S. marianum caused CCAP level in the midgut of the insect to decrease. Feeding on artificial diet containing methanolic extract of S. marianum inhibits release of CCAP in the midgut and leads to reduction of α-amylase and protease activities. | ||
| کلیدواژهها | ||
| Silybum marianum؛ Helicoverpa armigera؛ crustacean cardioactive peptide؛ Digestive enzymes؛ nutritional indices | ||
| عنوان مقاله [English] | ||
| اثر عصاره متانولی ماریتیغال بر شاخصهای تغذیهای، نوروپپتید سی سی ا پی و فعالیت آلفا آمیلاز و پروتئازکرم غوزه پنبه | ||
| نویسندگان [English] | ||
| وحید محمدی گیسور؛ اعظم میکانی؛ سعید محرمی پور | ||
| دانشکده کشاورزی دانشگاه تربیت مدرس | ||
| چکیده [English] | ||
| اثرات عصاره متانولی بذر ماریتیغال Silybum marianum روی لارو سن سه کرم غوزه پنبه Helicoverpa armigera (Hübner) در قالب رژیم غذایی مصنوعی در دمای 1±25 درجه، رطوبت نسبی 5 ±65 درصد و دوره نوری 8 :16 مورد بررسی قرار گرفت. LC50 و LC20 عصاره ماریتیغال به ترتیب برابر 10449 و 5654 پیپیام بود. صد میکرولیتر از عصاره گیاهی به غلظت 5654 پی پی ام (LC20) به نه دهم گرم غذای مصنوعی اضافه شده و پس از گذشت 72 ساعت، شاخصهای تغذیهای، مقدار نوروپپتید crustacean cardioactive peptide (CCAP) و میزان فعالیت آلفا آمیلاز و پروتئاز مورد بررسی قرار گرفت. عصاره گیاهی شاخصهای تغذیهای از جمله شاخص تقریبی هضم شوندگی غذا، نرخ رشد نسبی، نرخ مصرف نسبی ، کارایی تبدیل غذای خورده شده و کارایی تبدیل غذای هضم شده را کاهش داد. عصاره گیاهی میزان فعالیت آنزیم آلفا آمیلاز و پروتئاز را در معده میانی کرم غوزه پنبه کاهش داد. فعالیت آنزیم آلفا آمیلاز در شاهد mU 194 بوده در حالیکه در تیمار mU8/86 اندازه گیری شد. میزان فعالیت آنزیم پروتئاز نیز از mU 2/108 در شاهد به 6/60 mU در تیمار رسید. . قرار دادن معده میانی در بافر حاوی CCAP منجر به افزایش فعالیت آلفا آمیلاز و پروتئاز شد در حالیکه بافر به تنهایی هیچ تاثیری نداشت. تغذیه از غذای مصنوعی حاوی عصاره گیاهی میزان نوروپپتید CCAP را در معده میانی لارو کاهش میدهد. در کل میتوان نتیجه گرفت که تغذیه از غذای مصنوعی حاوی عصاره ماریتیغال باعث کاهش آزاد سازی نوروپپتید CCAP در معده میانی شده که این کاهش به نوبه خود منجر به کاهش فعالیت آلفا آمیلاز و پروتئاز می شود. | ||
| کلیدواژهها [English] | ||
| ماریتیغال, کرم غوزه پنبه, crustacean cardioactive peptide, آنزیمهای گوارشی, شاخصهای تغذیهای | ||
| مراجع | ||
|
Baskar, K., Maheswaran, R., Kingsley, S. & Ignacimuthu, S. (2010) Bioefficacy of Couroupita guianensis(Aubl) against Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae) larvae. Spanish Journal of Agricultural Research 8, 135–141. Brantley, S.J., Oberlies, N. H., Kroll, D. J. and Paine, M.F. ( 2010) Two Flavonolignans from Milk Thistle (Silybum marianum) Inhibit CYP2C9-Mediated Warfarin Metabolism at Clinically Achievable Concentrations. The Journal of Pharmacology and Experimental Therapeutics 332, 1081–1087. Bues, R., Bouvier, J.C. & Boudinhon, L. (2005) Insecticide resistance and mechanisms of resistance to selected strains of Helicoverpa armigera (Lepidoptera: Noctuidae) in the south of France. Crop Protection 24, 814–820. Caasi-Lit, M.T. (2005) Effects of crude and partially purified extracts from UV-B-irradiated rice leaves on Helicoverpa armigera (Hübner). Photochemistry and Photobiology 81, 1101–1106. Charleston, D.S., Kfir, R., Vet, L.E.M. & Dicke, M. (2005) Behavioural responses of diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) to extracts derived from Melia azedarach and Azadirachta indica. Bulletin of Entomological Research 95, 457–465. Elpidina, E.N., Vinokurov, K.S., Gromenko, V.A., Rudenskaya, Y.A., Dunaevsky, Y.E. & Zhuzhikov, D.P. (2001) Compartmentalization of proteinases and amylases in Nauphoeta cinerea midgut. Archive of Insect Biochemistry and Physiology 48, 206–216. Feng, X., Jiang, H., Zhang, Y., i He, W. & Zhang, L. (2012) Insecticidal activities of ethanol extracts from thirty Chinese medicinal plants against Spodoptera exigua (Lepidoptera: Noctuidae). Journal of Medicinal Plants Research 6, 1263–1267. Finney, D. J. (1971) Probit Analysis.3th ed. Cambridge University Press. London. Fouda, M.M.A. & Takeda, M. (2015) Distribution of Short Neuropeptide F-like Immunohistochemical Reactivity in the Brain and Midgut ofthe Terrestrial Isopod, Armadillidium vulgare (Latreille). American Journal of Life Sciences 3, 76–82. Fuse, M., Zhang, J.R., Partridge,E., Nachman, R.J., Orchard, I., Bendena, W.G. & Tobe, S.S. 1999. Effects of an allatostatin and a myosuppressin on midgut carbohydrate enzyme activity in the cockroach Diploptera punctate. Peptides 20, 1285–1293. Hasheminia S.M., JalaliSendi, J., TalebiJahromi K.H. & Moharramipour, S. (2011) The effects of Artemisia annua L. and Achillea millefolium L.crude leaf extracts on the toxicity, development, feeding efficiency and chemical activities of small cabbage Pieris rapae L. (Lepidoptera: Pieridae). Pesticide Biochemistry and Physiology 99, 244–249. Hasheminia, S.M., Jalali Sendi, J., T. Jahromi, K. & Moharramipour, S. (2013) Effect of milk thistle, Silybium marianum, extract on toxicity, development, nutrition, and enzyme activities of the small white butterfly, Pieris rapae. Journal of Insect Science. DOI: http://dx.doi.org/10.1673/031.013.14601. Kamaraj, C., Abdul Rahuman, A. & Bagavan, A. (2008) Screening for antifeedant and larvicidal activity of plant extracts against Helicoverpa armigera (Hübner), Sylepta derogata (F.) and Anopheles stephensi (Liston). Parasitology Research103, 1361–1368. Koul, O., Jain, M.P. & Sharma, V.K. (2000) Growth inhibitory and antifeedant activity of extracts from Melia dubia to Spodoptera litura and Helicoverpa armigera larvae. Indian Journal of Experimental Biology 38, 63–68. Maestro, J.L. & Bellés, X. (2006) Silencing Allatostatin Expression Using Double-Stranded RNA Targeted to Preproallatostatin mRNA in the German Cockroach. Archives of Insect Biochemistry and Physiology 62, 73–79. Muthusamy, B., Arumugam, E., Dhamodaran, K., Thangarasu, M., Kaliyamoorthy, K. & Kuppusamy, E. (2015) Bioefficacy of Caesalpinia bonducella extracts against tobacco cutworm, Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae). Journal of Coastal Life Medicine 3, 382–388. Park, J.H., Schroeder, A.J., Helfrich-Forster, C., Jakson, F.R., & Ewer, J.(2003) Targeted ablation of CCAP neuropeptide-containing neurons of Drosophila causes specific defects in execution and circadian timing of ecdysis behavior. Development 130, 2645–2656. Reiher, W., Shirras, C., Kahnt, J., Baumeister, S., Isaac, R. E., & Wegener, C. (2011) Peptidomics and peptide hormone processing in the Drosophila midgut. Journal of Proteome Research 10, 1881–1892. Sakai, T., Satake, H. & Takeda, M. (2006) Nutrient-induced α-amylase and protease activityis regulated by crustacean cardioactive peptide (CCAP) in the cockroach midgut. Peptides 27, 2157–2164. Shekari, M., Jalal Jalali Sendi, J., Etebari, K., Zibaee, A.& Shadparvar, A. (2008) Effects of Artemisia annua L. (Asteracea) on nutritional physiology and enzyme activities of elm leaf beetle, Xanthogaleruca luteola Mull. (Coleoptera: Chrysomellidae). Pesticide Biochemistry and Physiology 91, 66–74. Shorey, H. & Hale, R. (1965) Mass-rearing of the larvae of nine noctuid species on a simple artificial medium. Journal of Economic Entomology 58, 522–524. Stangier, J., Hilbich, C., Beyreuther, K., & Keller, R. 1987.Unusual cardioactive peptide (CCAP) from pericardial organs of the shore crab, Carcinus maenas. Procection Natural Academic Science USA 84, 575–579. Talekar, N.S., Open, R.T. & Hanson, P. (2006) Helicoverpa armigera management: a review of AVRDC's research on host plant resistance in tomato. Crop Protection 5, 461–467. Tamhane, V,A,, Chougule, N.P., Giri, A.P., Dixit, A.R., Sainani, M.N.& Gupta, V.S. (2005) In vivo and in vitro effect of Capsicum annum proteinase inhibitors on Helicoverpa armigera gut proteinases. Biochimistry and Biophysic Acta 1722, 156–167. Thushimenan, S., Baskaran, J., Baranitharan, M. & Jeyasankar, A. (2016) Laboratory investigation of Terminalia arjuna and Trachyspermum roxburghianum against groundnut pest, Helicoverpa armigera. Asian Journal of Pharmaceuticaland Clinical Research 9, 232–236. Walbbauer, G. P. (1968) The consumption and utilization of food by insects. Advances in Insect Physiology 5, 229–288. Warthen, J.R., Stokes, J.B., Jacobson, M., & Konzempel, M.P. (1984) Estimation of azadirachtin content in neem extracts and formulations, Journal of liquid Chromatography 7, 591–598. | ||
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