| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,527,998 |
| تعداد دریافت فایل اصل مقاله | 90,477,189 |
تنوع ژنتیکی برخی جمعیتهایهندوانه (Citrullus lanatus var. lanatus) بر اساس صفات مورفولوژیک و نشانگرهای ریزماهواره(ISSR) | ||
| نهال و بذر | ||
| مقاله 5، دوره 32، شماره 4، بهمن 1395، صفحه 507-525 اصل مقاله (454.57 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/spij.2017.113086 | ||
| نویسندگان | ||
| حسین نهتانی1؛ اسد معصومی اصل* 1؛ کاظم صباغ2؛ رضا امیری فهلیانی1 | ||
| 1گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه یاسوج | ||
| 2استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل | ||
| چکیده | ||
| به منظور بررسی تنوع ژنتیکی بین جمعیتهای هندوانه بومی ایران،24 جمعیت بومی به همراه یک رقم خارجی (دورگ F-1 شرکت ساکاتا) با استفاده از صفات مورفولوژیک و نشانگرهای ISSR مورد مطالعه قرار گرفتند. برای بررسی تنوع مورفولوژیکی، جمعیتها در مزرعه و در قالب طرح بلوکهای کامل تصادفی در سه تکرار کاشته شده و صفات مورفولوژیک آنها شامل تعداد میوه در بوته، طول بلندترین شاخه، وزن میوه، طول و عرض میوه و ضخامت پوست میوه اندازهگیری شد. نتایج تجزیه واریانس نشان داد که بین جمعیتها از نظر تمام صفات مورفولوژیک مورد بررسی اختلاف معنیدار وجود داشت. گروهبندی جمعیتها بر اساس صفات مورفولوژیک با استفاده از تجزیه کلاستر، آنها را در چهار گروه مجزا قرار داد. در بررسی تنوع با استفاده از نشانگر مولکولی، ده آغازگر مورد استفاده 87 مکان قابل امتیازبندی تولید کردند که از این تعداد 75 مکان چندشکل بودند. مقادیرPIC برای آغازگرهای مورد استفاده از 52/0 (آغازگر P4) تا 91/0 (آغازگر UBC864) متغیر بوده و میانگین آن 83/0 برآورد شد. دندروگرام حاصل از تجزیه کلاستر به روش UPGMA مبتنی بر ضرایب تشابه ژنتیکی جاکارد، جمعیتها را در هفت گروه قرار داد. کمترین و بیشترین فاصله ژنتیکی بین جمعیتهای کردستان و کرمانشاه و جمعیتهای مازندران و خراسان بود. نتایج این پژوهش نشان داد که نشانگرهای ISSR به طور مؤثری میتوانند برای مطالعه تنوع ژنتیکی جمعیتهای هندوانه بومی ایران استفاده شوند و از میان آغازگرهای استفاده شده، UBC864 و UBC823 مناسبترین آغازگرها برای مطالعات بعدی تشخیص داده شدند. | ||
| کلیدواژهها | ||
| هندوانه؛ جمعیتها؛ تنوع ژنتیکی؛ نشانگرهای ISSR | ||
| عنوان مقاله [English] | ||
| Genetic Diversity of some Populations of Watermelon (Citrullus lanatus var. lanatus) Based on Morphological Traits and Microsatellite Markers (ISSR) | ||
| نویسندگان [English] | ||
| H. Nohtani1؛ A. Masoumiasl1؛ K. Sabbagh2؛ R. Amiri Fahliani1 | ||
| 1respectively, Department of Agronomy and Plant Breeding, College of Agriculture, Yasouj University, Yasouj, Iran. | ||
| 2Assistant Professor, Department of Plant Protection, College of Agriculture, University of Zabol, Zabol, Iran. | ||
| چکیده [English] | ||
| For assessing the genetic diversity of Iranian populations of watermelon, 24 local populations together with one foreign cultivar (F-1 hybrid of Sakata Company) were studied using morphological traits and ISSR markers. Populations were grown in field in a randomized complete block design with three replications, and then morphological traits including fruit number in bush, the tallest banch length, fruit weight, fruit diameter and length and rind thickness were measured. Results of analysis of variance showed significant differences among all populations regarding the morphological traits. Cluster analysis based on morphological traits, divided populations in to four groups. In assessment of genetic diversity by molecular markers, ten used primers generated 87 scorable bands, which 75 sites were polymorphic. Polymorphic information content (PIC) of primers varied from 0.52 (marker P4) to 0.91 (marker UBC864) with anaverage of 0.83. Cluster analysis based on Jaccard genetic similarity coefficient, placed populations into seven main clusters. Minimum and maximum genetic distances were observed between populations of Kordestan and Kermanshah and Mazandaran and Khorasan, respectively. Generally the results of this study revealed that ISSR can be efficiently used for assessment of genetic diversity in watermelon populations native to Iran and UBC823 and UBC864 were recognized as the best primers forfiture studies. | ||
| کلیدواژهها [English] | ||
| watermelon, populations, Genetic diversity, ISSR markers | ||
| مراجع | ||
|
Abdemishani, S., Eshraghi, H., and Fasihi-Harandi A. 1997. Polymerase chain reaction and it`s applications in genetics and plant breeding. Proceedings of the 4th Agronomy and Plant Breeding Congress, Isfahan, Iran (in Persian). Alvandi, R., Etminan, A., Mohammadi, R., and Shooshtari, L.2015. Genetic diversity of durum wheat genotypes using agronomic characteristics and molecular markers. Seed and Plant Improvement Journal 31-1 (3): 441-458 (in Persian). Anderberg, M. R. 1973. Cluster Analysis for Applications. Academic Press, New York, USA. Anonymous 2013a. Production Yearbook. Available on: http://www.fao.org. Anonymous 2013b. Statistics of Agricultural Crops in 2011. Ministry of Agriculture Jihad, Department of Planning and Economy, Office of Statistics and Information Technology, Tehran, Iran (in Persian). Azizi, H., Bernousi, I., Abdollahi Mandoulakani, B., and Darvishzadeh R. 2012. Study of genetic structure and diversity in cultivated alfalfa populations (Medicago sativa L.) using ISSR markers. Journal of Modern Genetics 6(4): 61- 69 (in Persian). Bihamta, M. R., Naserian-Khiabani, B., and Zamani, M. J. 2009. Molecular Markers in Plant Genetics and Biotechnology. University of Tehran Press, Tehran, Iran (in Persian). Bisognin, D. A. 2002. Origin and evolution of cultivated cucurbits. Ciencia Rural, Santamaria 32(51): 715-723.
Dashchi, S., Abdollahi, B. M., Darvishzade, R., and Bernousi, I. 2012. Molecular similarity relationships among Iranian bread wheat cultivars and breeding lines using ISSR Marker. Notulae Botanicae Horti Agrobotanici Clojnapoca 40: 254-260. Fabriki-Ourang, S., Shams-Bakhsh, M., Jalali Javaran, M., and Ahmadi, J. 2009. Analysis of genetic diversity of Iranian melons (Cucumis melon L.) using ISSR markers. Iranian Biology Journal 22(2): 271- 281 (in Persian).
Farshadfar, E. 1998. Application of Biometrical Genetics in Plant Breeding, Vol. 1. Tagh-e- Bostan Press, Kermanshah, Iran (in Persian). Ghareyazie, B. 1997. Application of DNA markers in plant breeding. Key Article in the 4th Agronomy and Plant Breeding Congress, Isfahan, Iran (in Persian). Gichimu, B. M., Owuor, B. O., Mwai G. N., and Dida M. M. 2009. Morphological characterization of some wild and cultivated watermelon (Citrullus sp.) accession in Kenya. Arpn. Journal of Agricultural and Biological Science 4: 1990- 6145. Guerra-Sanz, J. M. 2002. Citrullus simple sequence repeats markers from sequence data bases. Molecular Ecology Notes 2: 223- 225. Hashizume, T., Shimamoto, I., and Hirai, M. 2003. Construction of a linkage map and QTL analysis of horticultural traits for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] using RAPD, RFLP and ISSR Markers. Theoretical and Applied Genetics 106: 779- 785. Huh, Y. C., Solmaz, I., and Sari, N. 2008. Morphological characterization of Korean and Turkish watermelon germplasm. pp. 327-333. In: Itrat, M. (ed.) Proceedings of the XIth Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae. INRA, Avignon, France. Jaccard, P. 1908. Nouvelles recherché sur la distribution florale. Bulletin Societe Vaud. Sciences Naturates 44: 223-270. Johnson, R. A., and Wichern, D. W. 2007. Applied Multivariate Statistical Analysis, 4th edition: Prentice Hall International, INC, New Jersey, USA. Karimi Shahri, M. R., Dehvari, V., Hajian Shahri, M., and Mokhtarian A. 2012. Genetic diversity of some grapevine cultivars of Khorasan Razavi province based on RAPD and ISSR markers. Seed and Plant Improvement Journal 28-1 (2):159-172 (in Persian). Kumar, L. S. 1999. DNA marker in plant improvement: An overview. Biotechnology Advances 17: 143-182.
Levi, A., and Thomas, C. E. 1999. An improved procedure for isolation of high quality DNA from watermelon and melon leaves. Cucurbit Genetics Cooperative Report 22: 41- 42.
Levi, A., Thomas, C. E., Newman, M., Reddy, O. U. K., Zhang, X., and Xu, Y. 2004. ISSR and AFLP markers differ among American watermelon cultivars with limited genetic diversity. Journal of the American Society for Horticultural Sciences 129(4): 553-558.
Levi, A., Thomas, C. E., Simmons, A. M., and Thies, J. 2005. Analysis based on RAPD and ISSR markers reveals closer similarities among Citrullus and Cucumis species than with Praecitrullus fistulosus (stocks) pangalo. Genetic Resources and Crop Evolution 52: 465-472.
Levi, A., Thomas, C. E., Wehner, T. C., and Zhandg, X. 2001. Low genetic diversity indicates the need to broaden the genetic base of cultivated watermelon. HortScience 36 (6): 1096- 1101.
Levi, A., Wechter, P., and Davis, A. 2008. EST- PCR markers represent ting watermelon fruit genes are polymorphic among watermelon heirloom cultivars sharing a narrow genetic base. Plant Genetic Resource 7(1): 16-32.
Mladenovic, E., Berenji, E., Ognjanov, V., Kraljevic-Balalic, M., Ljubojevic, M., and Cukanovic, J. 2008. Conservation and morphological characterization of bottle gourd for ornamental use. Proceedings of the 46th Croatain and 6th International Symposium on Agriculture, Opatija, Croatia. pp. 550-553.
Mujaju, C., Sehic, J., Werlemark, G., Garkava-Gstavsson, L., Fatih, M., and Nybom, H. 2010. Genetic diversity in watermelon (Citrullus lanatus) landraces from Zimbabwe reveald by RAPD and SSR markers. Hereditas 147(4): 142-153.
Narouei-Rad, M. R., Alladu, M., Ghasemi, A., and Fanaei, R. 2010. Genetic variation and heritability of local population of Sistan Watermelon. Iranian Journal of Horticultural Science 40(4): 95-103 (in Persian).
Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., and Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2: 225-238.
Stansfield, W. D. 1991. Theory and Problems in Genetics. McGraw-Hill, New York, USA.
Stepansky, A., Kovalski, I., Schaffer, A. A., and Prel-Treves, A. 1999. Variation in sugar levels and invertase activity in mature fruit representing a broad spectrum of Cucumis melo genotypes. Genetic Resources and Crop Evolution 46: 53-62.
Solmaz, I., Sari, N., and Aka-Kacar, Y. 2010. The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers. Genetic Resources and Crop Evolution 57: 763-771.
Zhang, X. P., Rhodes, B. B., and Shorupska, H. S.1994. RAPD molecular markers in watermelon. Cucurbit Genetics Cooperative Report 17: 116-119.
| ||
|
آمار تعداد مشاهده مقاله: 737 تعداد دریافت فایل اصل مقاله: 484 |
||