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Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 72، شماره 3، آذر 2017، صفحه 159-164 اصل مقاله (253.46 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.111610 | ||
| نویسندگان | ||
| S. A. Pourbakhsh* 1؛ A. R. Abtin1؛ A. Ashtari2؛ B. Kheirkhah3؛ M. A. Bayatzadeh2؛ Salimeh Ahangran4 | ||
| 1Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| 2Master | ||
| 3Assistant Professor | ||
| 4Bachelor | ||
| چکیده | ||
| Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis, which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease in goats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semen samples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented for M. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was applied to detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bp fragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed for both the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positive and typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. In addition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the samples were shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M. agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended to concern semen as one of the significant sources for this pathogen and the possibility for transmission to the female goats through semen is highlighted. Moreover, presence of this microorganism in semen could be involved in infertility of goat population. | ||
| کلیدواژه‌ها | ||
| Goat buck؛ Lipoprotein gene؛ Mycoplasma agalactiae؛ Semen؛ 16srRNA | ||
| عنوان مقاله [English] | ||
| جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بز | ||
| چکیده [English] | ||
| بیماری آگالاکسی یکی از عفونیترین بیماریهای شایع در گوسفند و بز میباشد. این بیماری از عفونتهای مایکوپلاسمایی محسوب میشود که معمولا همهگیر است. مایکوپلاسما آگالاکتیه بعنوان اصلیترین عامل این بیماری در بزها میباشد. هدف از این مطالعه جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بزهای نر بود. در مجموع 39 نمونه از اسپرم بزهای نر جمع آوری شد. تمام نمونه ها در محیط اختصاصی (PPLO broth) برای جداسازی مایکوپلاسما آگالاکتیه مورد کشت قرار گرفتند. پس از استخراج DNA باکتریها، آزمون واکنش زنجیرهای پلیمراز بر روی نمونه های استخراجی با استفاده از پرایمرهای اختصاصی با محصولاتی به طول 163 جفت باز در ژن 16S rRNA برای جنس مایکوپلاسما و 375 جفت باز در ژن لیپوپروتئین برای گونه مایکوپلاسما آگالاکتیه انجام شد. از مجموع 39 نمونه گرفته شده( %3/74)29نمونه مثبت بوده و دارای پرگنه در محیط کشت PPLOآگار بودند. (%4/97) 38 نمونه در آزمون واکنش زنجیرهای پلیمراز جنس مایکوپلاسما مثبت گزارش شدند و همچنین (%3/15)6 نمونه نیز در آزمون واکنش زنجیرهای پلیمراز گونه مایکوپلاسما آگالاکتیه مثبت تشخیص داده شدند که همگی آنها در آزمون واکنش زنجیرهای پلیمراز جنس مایکوپلاسما مثبت گزارش شده بودند. این مطالعه اولین گزارش جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بزهای نر در ایران میباشد. نتایج این مطالعه نشان داد که اسپرم میتواند محل مناسبی برای حضور این پاتو‍ژن و سپس انتقال آن به جنس ماده محسوب شود، لذا این عامل را میتوان به عنوان یکی از عوامل ناباوری در بزهای ماده محسوب نمود. | ||
| کلیدواژه‌ها [English] | ||
| مایکوپلاسما آگالاکتیه, بزهای نر, اسپرم, 16S rRNA, ژن لیپوپروتئین | ||
| مراجع | ||
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Abo-Elnaga, T.R., Gouda, A.S.A., Kamel, Y.M., Mahmoud, M.A., Osman, W.A., 2012. Application of the Polymerase Chain Reaction for identification of Mycoplasma isolated from aborted she camel. Glob Vet 8, 386-392. Ak, K., Ak, S., Gurel, M., Hasoksuz, A., Barau, Y., Ozturkeler, I., et al., 1995. Experimental studies on the effects of Mycoplasma agalactiae on the spermatozoa and genital organs of rams. Pendik Vet Mikrobiyol Derg 26, 139-155. Bergonier, D., Berthelot, X., Pourmarat, F., 1997. Contagious agalactia of small ruminants current of knowledge concerning epidemiology diagnosis and control. Rev Sci Techenol 16, 848-873. Bermudez, V.M., Miller, R.B., Rosendal, S., Fernando, M.A., Johnson, W.H., O'Brien, P.J., 1992. Measurement of the cytotoxic effects of different strains of Mycoplasma equigenitalium on the equine uterine tube using a calmodulin assay. Can J Vet Res 56, 331-338. Bielanski, A., Devenish, J., Phipps-Todd, B., 2000. Effect of Mycoplasma bovis Mycoplasma bovigenitalium in semen on fertilization and association with in vitro produced morula and blastocyst stage embryos. Theriogenology 53, 1213-1223. Breard, A., Poumarat, F., 1988. [Isolation of Mycoplasma capricolum from bull semen]. Rev Elev Med Vet Pays Trop 41, 149-150. Byrne, J., Brennan, P., McCormack, R., Ball, H.J., 1999. Isolation of <em>Mycoplasma bovis</em> from the abomasal contents of an aborted bovine fetus. Vet Record 144, 211. Corrales, J.C., Esnal, A., De la Fe, C., Sánchez, A., Assunçao, P., Poveda, J.B., et al., 2007. Contagious agalactia in small ruminants. Small Rumin Res 68, 154-166. de la Fe, C., Amores, J., Martin, A.G., Sanchez, A., Contreras, A., Corrales, J.C., 2009. Mycoplasma agalactiae detected in the semen of goat bucks. Theriogenology 72, 1278-1281. de la Fe, C., Gomez Martin, A., Amores, J., Corrales, J.C., Sanchez, A., Poveda, J.B., et al., 2010. Latent infection of male goats with Mycoplasma agalactiae and Mycoplasma mycoides subspecies capri at an artificial insemination centre. Vet J 186, 113-115. Dedieu, L., Mady, V., Lefevre, P.-C., 1995. Development of two PCR assays for the identification of mycoplasmas causing contagious agalactia. FEMS Microbiology Letters 129, 243-249. Gil, M.C., Peña, F.J., Hermoso De Mendoza, J., Gomez, L., 2003. Genital Lesions in an Outbreak of Caprine Contagious Agalactia Caused by Mycoplasma agalactiae and Mycoplasma putrefaciens. J Vet Med 50, 484-487. Givens, M.D., Marley, M.S., 2008. Pathogens that cause infertility of bulls or transmission via semen. Theriogenology 70, 504-507. Gomez-Martin, A., Corrales, J.C., Amores, J., Sanchez, A., Contreras, A., Paterna, A., et al., 2012. Controlling contagious agalactia in artificial insemination centers for goats and detection of Mycoplasma mycoides subspecies capri in semen. Theriogenology 77, 1252-1256. Hasso, S.A., Al-Aubaidi, J.M., Al-Darraji, A.M., 1993. Contagious agalactia in goats: It's severity as related to the route of infection and pregnancy. Small Rumin Res 10, 263-275. Jones, G.E., Rae, A.G., Holmes, R.G., Lister, S.A., Jones, J.M., Grater, G.S., et al., 1983. Isolation of exotic mycoplasmas from sheep in England. Vet Rec 113, 540. Jurmanova, K., Sterbova, J., 1977. Correlation between impaired spermatozoan motility and mycoplasma findings in bull semen. Vet Rec 100, 157-158. Kissi, B., Juhasz, S., Stipkovits, L., 1985. Effect of mycoplasma contamination of bull semen on fertilization. Acta Vet Hung 33, 107-117. Kojima, A., Takahashi, T., Kijima, M., Ogikubo, Y., Nishimura, M., Nishimura, S., et al., 1997. Detection of Mycoplasma in avian live virus vaccines by polymerase chain reaction. Biologicals 25, 365-371. Kotani, H., Nagatomo, H., Ogata, M., 1980. Isolation and Serological Comparison of Ureaplasmas from Goats and Sheep. Japanese J Vet Sci 42, 31-40. Lambert, M., 1987. Contagious agalactia of sheep and goats. Rev Sci Technol OIE 6, 699-711. Le Grand, D., Poumarat, F., Martel, J.L., 1995. [Genital Ureaplasma diversum infection: investigations in cattle in France]. Vet Res 26, 11-20. Miller, R., Chelmonska-Soyta, A., Smits, B., Foster, R., Rosendal, S., 1994. Ureaplasma Diversum As a Cause of Reproductive Disease in Cattle. Vet Clin North Am: Food Anim Prac 10, 479-490. Nicholas, R.A.J., Ayling, R.D., 2003. Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci 74, 105-112. Nunez-Calonge, R., Caballero, P., Redondo, C., Baquero, F., Martinez-Ferrer, M., Meseguer, M.A., 1998. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod 13, 2756-2761. OIE., 2008. Contagious agalactia Singh, N., Rajya, B.S., Mohanty, G.C., 1974. Granular vulvovaginitis (GVV) in goats associated with Mycoplasma agalactiae. Cornell Vet 64, 435-442. Spergser, J., Aurich, C., Aurich, J.E., Rosengarten, R., 2002. High prevalence of mycoplasmas in the genital tract of asymptomatic stallions in Austria. Veterinary Microbiology 87, 119-129. Sylla, L., Stradaioli, G., Manuali, E., Rota, A., Zelli, R., Vincenti, L., et al., 2005. The effect of Mycoplasma mycoides ssp. mycoides LC of bovine origin on in vitro fertilizing ability of bull spermatozoa and embryo development. Anim Reprod Sci 85, 81-93. Tola, S., Idini, G., Manunta, D., Galleri, G., Angioi, A., Rocchigiani, A.M., et al., 1996. Rapid and specific detection of Mycoplasma agalactiae by polymerase chain reaction. Vet Microbiol 51, 77-84
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