| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,208 |
| تعداد مقالات | 35,560 |
| تعداد مشاهده مقاله | 53,005,469 |
| تعداد دریافت فایل اصل مقاله | 92,289,422 |
Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin | ||
| Archives of Razi Institute | ||
| مقاله 5، دوره 72، شماره 3، آذر 2017، صفحه 181-187 اصل مقاله (190.79 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.111609 | ||
| نویسندگان | ||
| N. Razmaraii؛ H. Babaei1، 2؛ A. Mohajjel Nayebi2؛ S. Ahdi Khosroshahi3؛ S. Farajnia* 4 | ||
| 1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran | ||
| 2School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran | ||
| 3Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran | ||
| 4Drug Applied Research Center, Tabriz University of Medical Sciences | ||
| چکیده | ||
| Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN. | ||
| کلیدواژهها | ||
| Antioxidant activity؛ Crocin؛ Cytotoxicity؛ Grape Seed Extract؛ Phenytoin | ||
| مراجع | ||
|
Ahn, H.S., Jeon, T.I., Lee, J.Y., Hwang, S.G., Lim, Y., Park, D.K., 2002. Antioxidative activity of persimmon and grape seed extract: in vitro and in vivo. Nutr Res 22, 1265-1273.
Alavizadeh, S.H., Hosseinzadeh, H., 2014. Bioactivity assessment and toxicity of crocin: a comprehensive review. Food Chem Toxicol 64, 65-80.
Anderson, B.O., Yip, C.H., Smith, R.A., Shyyan, R., Sener, S.F., Eniu, A., et al., 2008. Guideline implementation for breast healthcare in low-income and middle-income countries: overview of the Breast Health Global Initiative Global Summit 2007. Cancer 113, 2221-2243.
Aune, D., Chan, D.S., Vieira, A.R., Rosenblatt, D.A.N., Vieira, R., Greenwood, D.C., Norat, T., 2012. Dietary compared with blood concentrations of carotenoids and breast cancer risk: a systematic review and meta-analysis of prospective studies. Am J Clin Nutr. 034165.
Balasundram, N., Sundram, K., Samman, S., 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence and potential uses. Food Chem 99, 191-203.
Bruno, E., Gargano, G., Villarini, A., Traina, A., Johansson, H., Mano, M.P., et al., 2016. Adherence to WCRF/AICR cancer prevention recommendations and metabolic syndrome in breast cancer patients. Int J Cancer 138, 237-244.
Correa, J.D., Queiroz-Junior, C.M., Costa, J.E., Teixeira, A.L., Silva, T.A., 2011. Phenytoin-induced gingival overgrowth: a review of the molecular, immune and inflammatory features. ISRN Dent 2011, 497850.
Dinicola, S., Cucina, A., Antonacci, D., Bizzarri, M., 2014. Anticancer effects of grape seed extract on human cancers: a review. J Carcinogenesis MutaS8: 005 .
Duan, X.J., Zhang, W.W., Li, X. M., Wang, B.G., 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem 95, 37-43.
Ferlay, J., Shin, H.R., Bray, F., Forman, D., Mathers, C., Parkin, D.M., 2010. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127, 2893-2917.
Fernández, J.A., 2006. Anticancer properties of saffron, Crocus sativus Linn. Advances Phytomedicine 2, 313-330.
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., et al., 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113, 71-88.
Kumar, S., Pandey, A.K., 2013. Chemistry and Biological Activities of Flavonoids: An Overview. Sci World J 2013, 1-17.
Mazzio, E.A., Soliman, K.F., 2009. In vitro screening for the tumoricidal properties of international medicinal herbs. Phytotherapy Res 23, 385-398.
Mousavi, S.H., Moallem, S.A., Mehri, S., Shahsavand, S., Nassirli, H., Malaekeh-Nikouei, B., 2011. Improvement of cytotoxic and apoptogenic properties of crocin in cancer cell lines by its nanoliposomal form. Pharm biol 49, 1039-1045.
Nassiri-Asl, M., Hosseinzadeh, H., 2009. Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds. Phytotherapy Res: PTR 23, 1197-1204.
Newman, D.J., Cragg, G.M., 2007. Natural Products as Sources of New Drugs over the Last 25 Years⊥. J Nat Prod 70, 461-477.
Pereira, C.A., Alchorne Ade, O., 2010. Assessment of the effect of phenytoin on cutaneous healing from excision of melanocytic nevi on the face and on the back. BMC Dermatol 10, 7.
Razmaraii, N., Babaei, H., Mohajjel Nayebi, A., Assadnassab, G., Ashrafi Helan, J., Azarmi, Y., 2016a. Crocin treatment prevents doxorubicin-induced cardiotoxicity in rats. Life Sci 157, 145-151.
Razmaraii, N., Babaei, H., Nayebi, A.M., Asadnasab, G., Helan, J.A., Azarmi, Y., 2016b. Cardioprotective Effect of Phenytoin on Doxorubicin-Induced Cardiac Toxicity in a Rat Model. J Cardiovasc Pharmacol 69, 237-245.
Rizzon, P., Di Biase, M., Favale, S., Visani, L., 1987. Class 1B agents lidocaine, mexiletine, tocainide, phenytoin. Eur Heart J 8, 21-25.
Sen, S., Chakraborty, R., 2011. The role of antioxidants in human health. Oxidative stress: diagnostics, prevention and therapy 1083, 1-37.
Sung, J., Lee, J., 2010. Antioxidant and antiproliferative activities of grape seeds from different cultivars. Food Sci Biotechnol 19, 321-326.
World Health Organization, 2002. WHO traditional medicine strategy 2002-2005.
World Health Organization, 2014. Estimated cancer incidence, mortality and prevalence worldwide in 2012. | ||
|
آمار تعداد مشاهده مقاله: 2,374 تعداد دریافت فایل اصل مقاله: 2,969 |
||