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بررسی مولکولی خلوص لاین‌های CMS برنج | ||
| نهال و بذر | ||
| مقاله 10، دوره 28، شماره 1، فروردین 1391، صفحه 149-153 اصل مقاله (149.72 K) | ||
| شناسه دیجیتال (DOI): 10.22092/spij.2017.111097 | ||
| نویسندگان | ||
| غفار کیانی* ؛ قربانعلی نعمت‌زاده | ||
| عنوان مقاله [English] | ||
| Molecular Purity Assessment of Rice CMS Lines | ||
| نویسندگان [English] | ||
| G. Kiani؛ G. Nematzadeh | ||
| چکیده [English] | ||
| The cytoplasmic male sterile (CMS) lines that are used in production of hybrid seeds in three-line system often get contaminated with their maintainer lines. Using such impure CMS lines in rice hybrid seed production lead to un-uniformity and yield loss in hybrid rice. Laborious and time consuming phenotypic evaluations are used for elimination of off-type plants in CMS multiplication farms. The other option for off-type elimination in CMS lines is application of molecular markers. In this study, PCR-based mitochondrial markers was used for distinguishing five male sterile lines from five maintainer lines. Results showed that marker cms had ability to differentiate male sterile lines Neda A and Nemat A from their maintainers. Also, marker drrcms differentiated male sterile lines Dasht A, Champa A and Amol 3 A from their respective maintainers. Results of the study demonstrate use of these two markers for off-type detection in CMS seed multiplication process. This is helpful in promotion of hybrid seed production technology in Iran. | ||
| کلیدواژه‌ها [English] | ||
| Key words: Rice, mitochondrial markers, male sterility, genetic purity | ||
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آمار تعداد مشاهده مقاله: 229 تعداد دریافت فایل اصل مقاله: 173 |
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