Related Links
The genetic characteristics of Marghos goat based on Cytochrome B gene | ||
| علوم دامی | ||
| Article 12, Volume 29, Issue 113, March 2017, Pages 135-140 PDF (677.79 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/asj.2017.109470 | ||
| Authors | ||
| M. Abdolmaleki; K. Pahlavan Afshar; hamid reza seyedabadi* | ||
| Abstract | ||
| Preservation of genetic diversity in population of Marghos goat is very important. Studying cytochrome b region of mitochondrial genome in within breeds can give useful information about genetic diversity of the population. The purpose of current research was genetic and phylogenetic investigation of mitochondrial genome cytochrome b region in Marghos goat. In order to, blood samples were collected from 40 Marghos goats. After extracting DNA, fragment 892 bp of cytochrome b region genome mitochondrial amplified by primers was designed and fragment amplified after purification was sequenced. Four different haplotypes were determined based on four single nucleotide polymorphism sequences. The haplotype and nucleotide diversity were estimated in Marghos goat population 0.1 and 0.00239 respectively. Using the UPGM phylogenetic test results showed that the Marghos goat population is separate group from other Asian goat breeds. The reason for this gap may be due to the geographic distance between the distributions of in Marghos goat with other breeds goats. | ||
| Keywords | ||
| Cytochrome b; Marghos goat; Mitochondrial genome; phylogeny | ||
| References | ||
|
توکلیان، ج. (1387). نگرشی بر ذخایر ژنتیکی دام و طیور ایران. انتشارات موسسه تحقیقات علوم دامی کشور.ص ص. 205-209. جوانروح علی آباد، ع.(1381). بررسی تنوع ژنتیکی شش توده بز بومی ایران با استفاده از نشانگر RAPD. پایان نامه کارشناسی ارشد. دانشکده کشاورزی، دانشگاه تربیت مدرس. نژاد گشتی، م. (1383). بررسی مولکولی تعدادی از نشانگرهای ریزماهواره در شش توده بز بومی ایران. پایان نامه کارشناسی ارشد. دانشگاه آزاد اسلامی واحد کرج. Bellagamba, F., Moretti, V.M., Comincini, S. and Valfare, F. (2001). Identification of species in animal Feedstuffs by polymerase chain reaction restriction fragment length polymorphism analysis of Mitochondrial DNA. Journal of Agricultural and Food Chemistry. 49: 3775-3781. Chen, S., Fan, B., Liu, B., Yu, M. and Zhao, S. (2006). Genetic variations of 13 indigenous Chinese goat breeds based on cytochrome b gene sequences. Biochemical Genetics. 44: 87-97. Eyre-walker, A. and Awadalla, P. (2001). Does human mtDNA recombine. Journal of Molecular Evolution. 53: 430-435. Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 41: 95–98. Luikart, G., Gielly, L., ExcoYer, L., Vigne, J. D., Bouvet, J. and Taberlet, P. (2001). Multiple origins and weak phylogeographic structure in domestic goats. Proceedings of the National Academy of Sciences. 98: 5927–5932. Miller, S.A., Dykes, D.D. and Polesky, H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research. 16: 1215. Mohammadi Pestehbig, F., Pirani, N., Shoja, J. and Mohammadhashemi, A. (2011). Determination the mtDNA D-loop Sequence in Marandi Native Chicken Population and Its Phylogenic Relationships with Other Breeds. Research Journal of Animal Sciences. 21: 1-9. Nei, M. and Kumar, S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York. Nei, M. (1987). Molecular Evolutionary Genetics .Columbia Univ Press, NewYork. Pakpahan, S., Tunas Artama, W., Widayanti, R. and Suparta, G. (2016) .Genetic Characteristics and Relationship in Different Goat Populations of Indonesia Based on Cytochrome B Gene Sequences. Asian Journal of Animal Sciences.10:29-38. Pirani, N., Mohammadhashemi, A., Alijani, S., Rezazadeh Goli, R. and Ghanbari, S. (2010). Molecular Analysis of Mazandrani native chicken population based on HVR-I region of Mitochondrial DNA. Journal Agriculture Biotechnology. 1: 53-60. Sutopo, J. and Kurnianto, E. (2014). The genetic diversity of Kejobong goat based on Cytochrome B gene.Journal of the Indonesian. Tropical Animal Agriculture. 39: 75-82. Takada, T., Kikkawa, Y., Yonekawa, H., Kawakami, S. and Amano, T. (1997). Bezoar (C. aegagrus) is a matriarchal candidate for ancestor of domestic goat (C. hircus): evidence from the mitochondrial DNA diversity. Biochemical Genetics. 35: 315-326. Tamura, K., Stecher, G., Peterson, D., Filipski A. and Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30: 2725-2729. | ||
|
Statistics Article View: 1,061 PDF Download: 778 |
||
Journal management system. designed by sinaweb