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بررسی اثر غلظتهای مختلف تنظیمکننده رشد گیاهی و نوع ریزنمونه بر میزان القای کالوس، رویانزایی و باززایی شاخساره مرزنجوش بخارایی (Origanum vulgare ssp. gracile) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 10، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 264-276 اصل مقاله (378.56 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109416 | ||
| نویسندگان | ||
| بهمن حسینی* 1؛ افسانه بیقامت2 | ||
| 1دانشیار، علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه | ||
| 2کارشناس ارشد گیاهان دارویی، علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه | ||
| چکیده | ||
| مرزنجوش بخارایی (Origanum vulgar ssp. gracile) از گیاهان دارویی متعلق به خانواده نعناعیان (Lamiaceae) میباشد. ترکیب غالب اسانس آن را کارواکرول تشکیل میدهد. در این تحقیق تأثیر غلظتهای مختلف 2,4-D در ترکیب با BAP بر روی کالوسزایی ریزنمونههای مختلف (کوتیلدون، هیپوکوتیل، گره، نوک شاخه و برگ) گیاه مرزنجوش بخارایی بررسی شد. اثر متقابل نوع محیط کشت در نوع ریزنمونه در سطح احتمال 1 درصد معنیدار شد. در ریزنمونه برگ حداکثر القای کالوس (100 درصد) در تیمارهای 1 و 3 میلیگرم در لیتر 2,4-D و در ریزنمونه گره در تیمار 3 میلیگرم در لیتر 2,4-D حداکثر درصد القای کالوس (3/93 درصد) مشاهده شد. تیمار 1/0 میلیگرم در لیتر BAP کمترین میزان القای کالوس (صفر درصد) را در ریزنمونه برگ نشان داد. بیشترین درصد تولید کالوس رویانزا در محیط کشت MS حاوی 1 میلیگرم در لیتر 2,4-D در ترکیب با 1/0 میلیگرم در لیتر BAP در ریزنمونه نوک شاخه بهدست آمد و کمترین میزان در محیط کشت MS بدون تنظیمکننده رشد گیاهی و محیط حاوی 1/0 میلیگرم در لیتر BAP در همه ریزنمونهها و در محیط حاوی 5 میلیگرم در لیتر 2,4-D در ریزنمونه هیپوکوتیل مشاهده شد. با افزایش غلظت تنظیمکننده رشد گیاهی 2,4-D رویانزایی کاهش یافت و در بین ریزنمونهها نوک شاخه و گره بالاترین درصد رویانزایی را داشتند. در آزمایش بررسی اثر BAP در شاخهزایی ریزنمونهها، بیشترین میزان شاخهزایی (100 درصد) در ریزنمونه گره و نوک شاخه در هر دو تیمار صفر و 1/0 میلیگرم در لیتر BAP بهدست آمد. | ||
| کلیدواژهها | ||
| اسانس؛ بنزیلآمینو پورین؛ توفوردی؛ وزن تر | ||
| عنوان مقاله [English] | ||
| Effects of different concentrations of growth regulators and explants type on callus induction, embryogenesis and shoot regeneration of Origanum vulgare ssp. Gracile | ||
| نویسندگان [English] | ||
| B. Hosseini1؛ A. Bighamat2 | ||
| 1Associate Prof., Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran | ||
| 2M.Sc., Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran | ||
| چکیده [English] | ||
| Origanum vulgar ssp. gracile is one of medicinal plants belonging to Lamiaceae family. Carvacrol was detected as a dominant component of essential oils of the species. Effects of different concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) in combination with benzyl adenine (BAP) were investigated on callus induction of various explants (Cotyledon, Hypocotyl, Node, Shoot Tip and leaf) of the species.Interaction between culture media and explant types was significant at 1% Level of probability. The highest percentage of callus induction (100%) was obtained on MS medium supplemented with 1 and 3 mg l−1 2,4-D on node explants. Maximum callus induction (93.3%) was observed on MS medium supplemented with 3 mg l−1 2,4-D. No callus induction (0%) was observed on control and MS medium supplemented with 0.1 mg l−1 BA on leaf explants. Higher percentage of embryogenic callus production was obtained on MS medium supplemented with 1 mg l−1 2,4-D in combination with 0.1 mg l−1 BAP on shoot tip explants. Percentage of embryogenic induction was the lowest on hormone free MS medium and media containing 0.1 mg l−1 BAP in all explants and MS media supplemented with 5 mg l−1 2,4-D on hypocotyl explant. By increasing 2,4-D concentration, embryogenesis was decreased and maximum embryogenesis percentage was obtained by shoot tip and node explants. Effects of BAP on shoot regeneration from explants, the highest shoot regeneration (100%) on nodes and shoot tip explants on hormone free media and MS media fortified with 1 mg l−1 BAP were obtained. | ||
| کلیدواژهها [English] | ||
| BAP, essential oil, fresh weight, 2, 4-D | ||
| مراجع | ||
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