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بررسی اثر فلز روی بر بیان ژن لیمونن سینتاز در زیره سبز با PCR در زمان واقعی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 3، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 190-198 اصل مقاله (228.58 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109409 | ||
| نویسندگان | ||
| محمد طغرلی1؛ جعفر وطندوست* 2؛ ملک حسین اسدی3 | ||
| 1دانشجوی کارشناسی ارشد، بیوتکنولوژی کشاورزی، دانشگاه آزاد اسلامی، واحد سبزوار | ||
| 2نویسنده مسئول مکاتبات، استادیار، گروه زیستشناسی، دانشگاه حکیم سبزواری، سبزوار | ||
| 3استادیار، گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان | ||
| چکیده | ||
| روی بهعنوان یک عنصر ضروری برای رشد و نمو گیاهان، نقش مهمی در بسیاری از فرایندهای متابولیکی گیاهان ازجمله بیان ژن لیمونن سینتاز برعهده دارد. لیمونن سینتاز بهعنوان یک آنزیم کلیدی در مسیر بیوسنتز روغنهای فرار میباشد که در واکنشهای دفاعی گیاهان نقش دارد. از اینرو در این پژوهش اثر غلظتهای مختلف روی بر بیان ژن لیمونن سینتاز در زیره سبز و در قالب طرح کاملاً تصادفی بررسی شد. بعد از استخراج RNA و سنتز cDNA، بیان ژن با روش Real time PCR بررسی شد. نتایج مقایسه میانگین میزان بیان ژن با آزمون چند دامنهای دانکن در غلظتهای مختلف نشان داد که در گیاهان تیمار شده، افزایش غلظت روی تا 100 میکرومولار با افزایش بیان ژن لیمونن سینتاز رابطه مستقیم دارد. با توجه بهنقش آنزیم لیمونن سینتاز، افزایش بیان آن میتواند از طریق افزایش تولید مونوترپنها در گیاه باعث تولید بیشتر روغنهای فرار و در نتیجه محافظت گیاه در مقابل علفخوارها شود. | ||
| کلیدواژهها | ||
| بیان ژن؛ روی؛ زیره سبز؛ لیمونن سینتاز؛ real time PCR | ||
| عنوان مقاله [English] | ||
| Study of zinc effect on limonene synthase gene expression in cumin by Real Time PCR | ||
| نویسندگان [English] | ||
| Mohammad Toghroli1؛ Jafar Vatandoost2؛ M.H. Asadi3 | ||
| 1M.Sc., Department of Biotechnology, Faculty of Agriculture, Islamic Azad University, Sabzevar, I.R. Iran | ||
| 2Corresponding author Assis. Prof., Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, I.R. Iran, E-mail: j.vatan@hsu.ac.ir | ||
| 3Assis. Prof., Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. Iran. | ||
| چکیده [English] | ||
| Zinc as an essential element for plant growth, plays an important role in many metabolic processes of plants, such as limonene synthase gene expression pathway. Limonene synthase is considered as an important enzyme in volatile oil biosynthesis that plays significant role in plant defensive reactions. In the present study, limonene synthase gene expression in Cumin was evaluated in presence of different concentration of Zinc elicitors based on a completely randomized design. Total RNA was extracted and cDNA synthesis was performed and Real Time PCR quantity assay method was used for limonene synthase gene expression. Results of the comparison of gene expression with Duncan's multiple range tests in different concentrations showed that in treated plants, there was a direct correlation between increasing of Zinc concentration up to 100 µM and limonene synthase gene expression. Limonene synthase gene expression by increasing production of monoterpens may produce more volatile oils in plant species and thus protects the plant against herbivores. | ||
| کلیدواژهها [English] | ||
| Cuminum cyminum, Gene expression, limonen synthase, real time PCR, Zinc | ||
| مراجع | ||
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-Behera, S., Nagarajan, S. and Jagan Mohan Rao, L., 2004. Microwave heating and conventional roasting of cumin seeds (Cuminum cyminum L.) and effect on chemical composition of volatiles. Food chemistry, 87: 25-29. -Brown, P.H., Cakmak, I. and Zhang, Q., 1993. Form and function of zinc plants. Springer, 93-106. -Dorling, J., Leung, S., Anderson, C., Albert, W. and McManus, M., 2011. Changes in 1-aminocyclopropane-1-carboxlate (ACC) oxidase expression and enzyme activity in response to excess manganese in white clover (Trifolium repens L.). Plant Physiology and Biochemistry, 49: 1013-1019. -Dudareva, N., Negre, F., Nagegowda, D. and Orlova, I., 2006. Plant volatiles: recent advances and future perspectives. Critical Reviews in Plant Sciences, 25: 417-440. -Gershenzon, J., McConkey, M. and Croteau, R., 2000. Regulation of monoterpene accumulation in leaves of peppermint. Plant Physiology, 122: 205-214. -Ghannadnia, M., Haddad, R., Zarinkamar , F. and Sharifi, M. , 2011. Different expression of limonene synthase gene in organs and developmental stages of cumin (Cuminum cyminum L.). Iranian Journal of Medicinal and Aromatic Plants, 27: 495-501. -Ghorbanli, M. , Ahmadi, F., Monfared, A. and Bakhshi Khaniki, Gh., 2012. Effect of salt stress and its interaction with ascorbate on catalase, ascorbate peroxidase activity, proline and malondialdehyde in Cuminum cyminum L.four weeks after germination. Iranian Journal of Medicinal and Aromatic Plants, 28: 14-21. -Ibrahim, M., Kainulainen, P. and Aflatuni, A., 2008. Insecticidal, repellent, antimicrobial activity and phytotoxicity of essential oils: with special reference to limonene and its suitability for control of insect pests. Agricultural and Food Science, 10: 243-259. -Kan, Y., Kartal, M., Ozek, T., Aslan, S. and Baser, K., 2007. Composition of essential oil of Cuminum cyminum L. according to harvesting times. Turkish Journal of Pharmaceutical Sciences, 1: 25-29. -Kjonaas, R. and Croteau, R., 1983. Demonstration that limonene is the first cyclic intermediate in the biosynthesis of oxygenated p-menthane monoterpenes in Mentha piperita and other Mentha species. Archives of Biochemistry and Biophysics, 220: 79-89. -Lücker, J., Schwab, W., van Hautum, B., Blaas, J., van der Plas, L.H.W., Bouwmeester, H.J. and Verhoeven, H.A., 2004. Increased and altered fragrance of tobacco plants after metabolic engineering using three monoterpene synthases from lemon. Plant Physiology, 134: 510-519. -Mahmoud, S., Williams, M. and Croteau, R., 2004. Cosuppression of limonene-3-hydroxylase in peppermint promotes accumulation of limonene in the essential oil. Phytochemistry, 65: 547-554. -Muñoz-Bertomeu, J., Ros, R., Arrillaga, I. and Segura, J., 2008. Expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves. Metabolic Engineering, 10: 166-177. -Rion, B. and Alloway, J., 2004. Fundamental aspects of zinc in soils and plants, International Zinc. Association, 1-128. -Srivastava, K., 1989. Extracts from two frequently consumed spices cumin (Cuminum cyminum) and turmeric (Curcuma longa) inhibit platelet aggregation and alter eicosanoid biosynthesis in human blood platelets. Prostaglandins, Leukotrienes and Essential Fatty Acids, 37: 57-64. -Takayuki, S., Sugano, M., Majid, A. and Fujii, Y., 2007. Antifungal effects of volatile compounds from black zira (Bunium persicum) and other spices and herbs. Journal of Chemical Ecology, 33: 2123-2132. -Thippeswamy, N. and Naidu, K.A., 2005. Antioxidant potency of cumin varieties cumin, black cumin and bitter cumin on antioxidant systems. Journal of European Food Research and Technology, 220: 472-476. -Turner, G., Gershenzon, J., Nielson, E., Froehlich, J. and Croteau, R., 1999. Limonene synthase, the enzyme responsible for monoterpene biosynthesis in peppermint, is localized to leucoplasts of oil gland secretory cells. Plant Physiology, 120: 879-886. -Willer, C., Sanna, S., Jackson, A., Scuteri, A., Bonnycastle, L. and Stringham, H., 2008. Newly identified loci that influence lipid concentrations and risk of coronary artery disease. Nature Genetics, 40: 161-169. -Yousefi, K., Riahi, A. and Nasiri, M., 2011. Study of flavone synthase 1 (FNS1) gene expression in of cumin (Cuminum cyminum) in the presence of different concentrations of silver elicitors 12th Iranian Genetics Congress, Tehran. -Zarei, M. 2014. Study of limonene synthase gene expression in the presence of different copper concentrations in Cuminum cyminum L. M.Sc. Thesis, Azad University of Sabzevar, Sabzevar. -Zarinkamar, F., Ghannadnia, M. and Haddad, R., 2012. Limonene synthase gene expression under different concentrations of manganese in Cuminum cyminum L. African Journal of Plant Science, 6: 203-212. -Zwenger, S. and Basu, C., 2008. Plant terpenoids: applications and future potentials. Biotechnology and Molecular Biology Reviews, 3: 1-7. | ||
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