Production of pharmaceutical active ingredients via hairy root induction of Birch (Betula pendula) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 1, Volume 24, Issue 2 - Serial Number 48, December 2016, Pages 165-176 PDF (369.76 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2016.109403 | ||
| Authors | ||
| Razieh Jafari Hajati* 1; Vahideh Payamnoor2; Kamal Ghasemi Bezdi2; Najmeh Ahmadian Chashmi3 | ||
| 1Ph.D student, Faculty of forest sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran | ||
| 2Assis. Prof., Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran | ||
| 3Assis. Prof., Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I.R.Iran | ||
| Abstract | ||
| Betula pendula Roth produces a range of biologically active terpenoids such as betulin and betulinic acid. The compounds with cytotoxic effects which have been used for their medicinal properties. Agrobacterium-infected hairy roots are characterized by high growth rate and genetic stability that can beprovided noticeable amounts of the biological material. In this study, production of hairy roots by B. pendula was investigated on different explants, media and A. rhizogenes strains. Growth rate of hairy roots were studied during 20 days and the terpenoids content (betulin & betulinic acid) were assayed by HPLC. Hairy root cultures were established 20 days after infection by infecting bark segments of B. pendula by Agrobactrium strains of C58C1 and LB9402 in WPM medium. Growth of hairy root by lines of C58C1 strain (D and E lines) and one line from LB9402 strain (LB1) were significantly better than others. The most betulin content was observed in the hairy roots, 8 days after culture, which was about 0.47 mg g-1 dry waight (DW). Furthermore, the most amount of antioxidant activity (75%) was assessed on E line roots, 20 days after cultures. Although the highest content of betulinic acid (0.6 mg g-1 DW ) was obtained by LB1 lines but betulin content and antioxidant activity were considerably less than other hairy root lines. Results indicated that due to higher relative performance, E line of hairy roots could be used for analysis of terpenoids and pharmaceutically important metabolites in future studies. | ||
| Keywords | ||
| Antioxidant; Betula pendula; betulin; Betulinic acid; hairy root | ||
| References | ||
-Asghari, Gh., 2006. Biotechnological Production of Medicinal Plants and Herbal Medicines. Publications of Esfahan SID, 287p. Persian. -Bandeali, A., Keyhanfar, M. and Asghari, Gh., 2013. Improve the production of artemisinin in Artemisia annua Hairy roots using Staphylococcus aureus. Journal of Medicinal Plants, 4: 82-92. Persian. -Christey, M.C. and Braun, R.H., 2005. Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes mediated transformation. Methods in Molecular Biology, 286: 47 -60. -Cichewicz, R.H. and Kouzi, S.A., 2004. Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection. Medicinal Research Reviews, 24:90–114. -Doyle, J.J. and Doyle, J.L., 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 5-13. -Fan, G., Zhai, Q., Li, X. and Zhan, Y., 2013. Compound of Betula platyphylla cell suspension cultures in response to fungal elicitor. Biotechnol & Biotechnol, 27: 3569-3572. -Gamborg, O.L., Miller, R.A. and Ojima, K.,1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research. 50: 151-158. -Fett-Neto, A.G., Stewart, J.M., Nicholson, S.A., Pennington, J.J. and Di-Cosmo, F., 1994. Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of T. cuspidata. Biotechnology and Bioengineering, 44: 967-971. -Guillen, S., Tremouillaux-Guillen, J., Pati, P.K., Rideau, M. and Gantet, P., 2006. Harnessing the potential of hairy roots. Trends in Biotechnology, 24: 403-409. -Hassanloo, T., Rezazade, Sh. and Rahnama, H., 2008. Hairy roots as a source of compounds with medicinal value. Journal of Medicinal Plants. 29: 1-17. -Hiltunen, E., Pakkanen, T.T. and Alvila, L., 2006. Phenolic compounds in silver birch (Betula pendula Roth) wood. Holzforschung, 60: 519–527. -Hu, Z.B. and Du, M., 2006. Hairy root and its application in plant genetic engineeering. Journal of Integrative Plant Biology, 48: 121-127. -I.U.C.N., 2001. IUCN Red list categories and criteria. IUCN, Gland, Switzerland. -Kahkonen, M.P., Hopia, A. I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S. and Heinonen, M., 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47: 3954–3962. -Kim, J.A., Baek, K.H., Son, Y.M., Son, S.H. and Shin., H., 2009. Hairy Root Cultures of Taxus cuspidata for Enhanced Production of Paclitaxel. Journal of the Korean Society for Applied Biological Chemistry, 52:144-150. -Kokate, C.K., 2006. Medicinal Plant Biotechnology.CBS Publisher and Distributors, 506 pp. -Krasutsky, P.A., 2006. Birch bark research and development. Natural Product Reports, 23:919–942. -McCown, B.H. and Sellmer, J.C., 1982. Media and physical environment. In: Bonga, J.M. and Durzan, D.J (Eds.), Cell and Tissue Culture in Forestry, VoL 1, General Principles and Biotechnology. Martinus Nijhoff Publishers, Pordrecht. 422. -Martin, C., Parra, T., Clemente-Muñoz, M. and Hernandez-Bermejo, J.E., 2008. Genetic diversity and structure of the endangered Betula pendula subsp. fontqueri populations in the south of Spain. Silva Fennica, 42: 487–498. -Mehri Rad, N., 2014. Possibility to increase betulin extract of betula litwinowii callus In Vitro condition. MS thesis. Gorgan University of Agricultural Sciences and Natural Resources. Iran, MS thesis, 74p. Persian. -Nagata, I. and Takebe, I., 1971. Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12-20. -Omidi, M. and Farzin, N., 2012. Biotechnology applications to increase the effectiveness of medicinal plants. Modern Genetic, 7: 209-220. -Payamnoor, V. and Jafari Hajati, R., 2015.To assess changes of major secondary metabolites produced in the calli of Betula pendula by affecting expellant type and medium culture. Research project of Gorgan University of Agricultural Sciences and Natural Resources, Iran, No, 21-314-92, 31p. Persian. -Payamnoor, V., Jafari Hajati, R. and Nazari, J., 2015. Callogenesis of two species of birch (Bpendula and B litwinowii) using the bark explant and evaluation of inducted betulin. Reaserch project of Gorgan University of Agricultural Sciences and Natural Resources, Iran, No, 20-314-92, 21p. Persian. -Pisha, E., Chai, H., Lee, I.S., Chagwedera, T.E., Farnsworth, N.R., Cordell, A.C., Beecher, C.W.W., Fong, H.H.S., Kinghorn, A.D., Brown, D.M., Wani, M.C., Wall, M.E., Hieken, T.J., Das Gupta, T.K. and Pezzuto, J.M., 1995. Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis: Natural Medicines, 1: 1046-1051. -Rajasekaran, K., Hudspeth, R.L., Cary, J.W., Anderson, D.M. and Cleveland, T.F., 2000. High-frequency transformation of catton (Gossypium hirsutum L.) by particle bombardment of embrogenic cell suspension cultures. Plant Cell Report, 19: 539 - 45. -Simola, L.K., 1985. Propagation of plantlets from leaf callus of Betula pendula f. purpurea. Scientia Hortic, 26: 77-85. -Srivastava, P.S. and Steinhauer, A.,1981. Isozymes in differentiating shoot bud cultures of Betula pendula Roth. Zeitschrift für Pflanzenphysiologie, 103: 341-346. -Srivastiva, P.S., Steinhauer, A. and Glock, H., 1985. Plantlet differentiation in leaf and root cultures of birch (Betula Pendula Roth). Plant Science, 42:209-214. -Stephanie, G., Jocelyne, T.G., Pratap, K.P., Marc, R. and Pascal. G., 2006. Hairy root research:recent scenario and prospects. Current Opinion in Plant Biology, 9: 341-346. -Valko, M., Izakovic. M., Mazur, M., Rhodes, C.J. and Telser, J., 2004. Role of oxygen radicals in DNA damage and cancer incidence. Molecular Cell Biochemistry, (1-2): 37-56. -Zare, H., 2002. Ecologia study of Betula pendula stands in Sangde and Lar vally. Tarbiat Modares University. Iran, M.Sc. thesis, 140p. Persian. -Zhao, G., Yan, W.D. and Cao, D., 2007. Simultaneous determination of betulin and betulinic acid in white birch bark using RP–HPLC. Journal of Pharmaceutical and Biomedical, 43; 959–962. | ||
|
Statistics Article View: 1,162 PDF Download: 851 |
||
Journal management system. designed by sinaweb