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Growth Inhibitory Effect of Anthemis haussknechtii Root Extract, as a Source of Parthenolide, on Breast Cancer Cell Line | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 10، دوره 5، شماره 2، آذر 2016، صفحه 205-210 اصل مقاله (210.69 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2016.109397 | ||
| نویسندگان | ||
| Nesa Jafari1؛ Sonbol Nazeri* 2؛ Zohreh Rabiei3؛ Sattar Tahmasebi Enferadi3؛ Reza Behroozi3 | ||
| 1Department of Plant Biotechnology, Bu-Ali Sina University, Hamedan, Iran and Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | ||
| 2Department of Plant Biotechnology, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | ||
| چکیده | ||
| Parthenolide is major Sesquiterpene lactones present in Anthemis haussknechtii Boiss. & Reut. (feverfew). This compound has many effects on different disease such as migrain and cancer. Parthenolide was reported from Tanacetum parthenium (L.) Sch.Bip. but other plants of Asteraceae family could contain parthenolide. In this study parthenolide was extracted and identified with two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Breast cancer cell line, MDA-MB-231, was exposed to different concentrations of parthenolide for 24 hours. Half maximal inhibitory concentration (IC50) was evaluated using Methylthiazol Tetrazolium (MTT) test. Based on results, 1000 µg/ml concentration is the minimum lethal dose that kills approximately 50% of cells after 24 hours. The results revealed that the A. haussknechtii parthenolide dramatically decreased survival of cancer cell line by inducing apoptosis. This is the first report of cytotoxicity effect of A. haussknechtii extract on breast cancer cell line. | ||
| کلیدواژه‌ها | ||
| Cell Viability, FT-IR؛ HPLC, MTT test, Parthenolide | ||
| مراجع | ||
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1. Bessada F, Barreira C M, Oliveira P P. Asteraceae species with most prominent bioactivity and their potential applications: A review. Indust Crop Prod. 2015; 76:604-615
2. Izadpanah M, Seyadan E. Evaluation of genetic diversity of Anthemis houssknechtii and Anthemis altissima population through agro–morphological characteristics. J Biol Environ Sci. 2015;7:403-412
3. Majdi M, Liu Q, Karimzadeh G, Malboobi MA, Beekwilder J, Cankar K, Vos Rd, Todorović S, Simonović A, Bouwmeester H. Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip). Phytochemistry. 2011;72:1739-1750.
4. Bork P M, Schmitz1 M, Kuhnt M, Escher C, Heinrich M. Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-KB. FEBS Lett.1997;402:85-90.
5. Holliday DL, Speirs V. Choosing the right cell line for breast cancer research. Breast Cancer Res. 2011;13:215-222. 6. Saleem A, Husheem M, Harkonen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. J Ethnopharmacol. 2002;81:327–36. 7. Riva L, Coradini D, Di Fronzo G, De Feo V, De Tommasi N, De Simone F. The antiproliferative effects of Uncaria tomentosa extracts and fractions on the growth of breast cancer cell line. Anticancer Res. 2001; 21:2457-61. 8. Hostanska K, Nisslein T, Freudenstein J, Reichling J, Saller R. Cimicifuga racemosa extract inhibits proliferation of estrogen receptor-positive and negative human breast carcinoma cell lines by induction of apoptosis. Breast Cancer Res Treat. 2004; 84:151-60. 9. Shoemaker M, Hamilton B, Dairkee SH, Cohen I, Campbell MJ. in vitro anticancer activity of twelve Chinese medicinal herbs. Phytother Res. 2005;19:649-51. 10. Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005;100:72-79. 11. Sobhani AM, Ebrahimi SA, Mahmoudian M. An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its beta-carboline alkaloids. J Pharmacol Sci.2002;5:19-23. 12. Funayama Y, Nishio K, Wakabayashi K, Nagao M, Shimoi K, Ohira T, et al. Effects of beta- and gamma-carboline derivatives of DNA topoisomerase activities. Mutat Res. 1996; 349:183-91. 13. Abe A, Yamada H. Harmol induces apoptosis by caspase-8 activation independently of Fas/Fas ligand interaction in human lung carcinoma H596 cells. Anticancer Drugs. 2009; 20:373-81. 14. Sahler J, Bernard J J, Spinelli S L, Blumberg N, Phipps R P. The Feverfew plant-derived compound, parthenolide enhances platelet production and attenuates platelet activation through NF-κB inhibition. Thromb Res. 2011; 127:426-434.
15. Turska J P, Paduch R, Majdan M, Szerszeñ M K, Rzeski W. Antiproliferative activity of parthenolide against three human cancer cell lines and human umbilical vein endothelial cells .Pharmacol Reports. 2007;59:233-237.
16. Heydari M, Poorbabaei H, Hatami K, Salehi A , Begim Faghir M.Floristic study of Dalab woodlands, north-east of Ilam province, west Iran. IJST. 2013; 37:301-307.
17. Chehregani A, Mehanfar N. :New chromosome counts in the tibe Anthemideae(Astraceae) from Iran. Cytologia. 2008;73:1-8.
18. Cheng G, Xie L. Parthenolide Induces Apoptosis and Cell Cycle Arrest of Human 5637 Bladder Cancer Cells. In Vitro. Molecules. 2011;1:6758-6768.
19. Koprowska K, Czyż M. Molecular mechanisms of parthenolide’s action: Old drug with a new face . Postepy Hig Med Dosw(online). 2010; 64:100-114.
20. Czyz M, Lesiak-Mieczkowska K, Koprowska K, Szulawska-Mroczek A.and M Wozniak .Cell context-dependent activities of parthenolide in primary and metastatic melanoma cell. Brit J Pharmacol. 2010;160:1144-1157. | ||
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