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Considerable Azadirachtin Production in Neem Cell Culture under Abiotic Elicitor Induction | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 9، دوره 5، شماره 2، آذر 2016، صفحه 195-204 اصل مقاله (331.71 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2016.109396 | ||
| نویسندگان | ||
| Ghasemali Garoosi* ؛ Banafsheh Gholami؛ Ramin Hosseini | ||
| Biotechnology Deptartment, Imam Khomeini International University (IKIU), Qazvin, Iran | ||
| چکیده | ||
| In this study the effect of different concentrations of some abiotic elicitors (salicylic acid, jasmonic acid, cadmium chloride and sodium chloride) with different incubation times (3, 6, 9 and 12) on azadiractin production and cell growth in the cell culture of the Iranian native Neem were investigated. Cell growth rate was measured by changes in dry cell weight and it was used as a basis for the treatment of elicitor effect. Azadirachtin was extracted with methanol and measured by high performance liquid chromatography (HPLC). The results showed that the AZ content of cells increased significantly in the cells elicited with different types and concentrations of all used elicitors compared to the control. The cell suspension which treated with 0.75 mM salicylic acid showed the highest Azadirachtin content (0.1268 g/g DCW) 6 days after incubation. The highest dry cell weight, with a significant increase, was observed in the suspension cultures treated with 0.1 mM cadmium chloride 12 days after incubation. However, this study highlighted the new valuable Iranian native Neem genotype and salicylic acid as a powerful elicitor for azadirachtin production. In addition, it was approved the effect of exposure time, individual intrinsic and more presumably combined condition of elicitors and their concentration on plant secondary metabolites production. | ||
| کلیدواژهها | ||
| Azadirachtin؛ Cell suspension culture؛ Elicitor؛ Neem | ||
| مراجع | ||
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1. Tinghui X, Wegener MK, O'Shea M, Deling M: World distribution and trade in neem products with reference to their potential in China. 2001 Conference (45th), January 23-25, Adelaide, 2001. Australian Agricultural and Resource Economics Society. 2. Rodrigues M, Festucci-Buselli RA, Silva LC, Otoni WC:Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents. Braz Arch Biol Technol. 2014;57:155-162. 3. Dasgupta T, Banerjee S, Yadava PK, Rao AR:Chemopreventive potential of Azadirachta indica (Neem) leaf extract in murine carcinogenesis model systems. J Ethnopharmacology. 2004;92:23-36. 4. Butterworth JH, Morgan E:Isolation of a substance that suppresses feeding in locusts. Chem Commu (London). 1968;1:23-24. 5. Salehzadeh A, Akhkha A, Cushley W, Adams RLP, Kusel JR, Strang RHC:The antimitotic effect of the neem terpenoid azadirachtin on cultured insect cells. Insect Biochem Mol Biol. 2003;33:681-689. 6. Tiwari R, Verma AK, Chakraborty S, Dhama K, Singh SV:Neem (Azadirachta indica) and its potential for safeguarding health of animals and humans: A review. J Biol Sci. 2014;14:110. 7. Prakash G, Srivastava AK:Statistical elicitor optimization studies for the enhancement of azadirachtin production in bioreactor Azadirachta indica cell cultivation. Biochem Eng J. 2008;40:218-226. 8. Sidhu O, Behl H:Seasonal variation in azadirachtins in seeds of Azadirachta indica. Curr Sci. 1996;70:1084-1085. 9. Shukor NAA, Seong LK:Azadirachtin variation of six provenances of Azadimchm excelsa (Jack) Jacob. Pakistan J Biol Sci. 2006;9:833-836. 10. Sidhu O, Kumar V, Behl HM:Variability in neem (Azadirachta indica) with respect to azadirachtin content. J Agric Food Chem. 2003;51:910-915. 11. Baldi A, Dixit V:Enhanced artemisinin production by cell cultures of Artemisia annua. Curr Trends Biotechnol Pharm. 2008;2:341-348. 12. Satdive RK, Fulzele DP, Eapen S:Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A. Juss by elicitation and media optimization. J Biotechnol. 2007;128:281-289. 13. Zhao J-L, Zhou L-G, Wu J-Y:Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. App Microbiol Biotechnol. 2010;87:137-144. 14. 14. Zare N, Farjaminezhad R, Asghari-Zakaria R, Farjaminezhad M:Enhanced thebaine production in Papaver bracteatum cell suspension culture by combination of elicitation and precursor feeding. Nat Prod Res. 2014;28:711-717. 15. 15. Prakash G, Srivastava AK:Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures. Process Biochem. 2005;40:3795-800. 16. Prakash G, Srivastava AK:Modeling of azadirachtin production by Azadirachta indica and its use for feed forward optimization studies. Biochem Eng J. 2006; 29:62-68. 17. Dai J, Yaylayan VA, Vijaya Raghavan G, Pare JR, Liu Z:Multivariate calibration for the determination of total azadirachtin-related limonoids and simple terpenoids in neem extracts using vanillin assay. J Agric Food Chem. 2001;49:1169-1174. 18. Smith JL, De Moraes CM, Mescher MC:Jasmonate- and salicylate‐mediated plant defense responses to insect herbivores, pathogens and parasitic plants. Pest Manag Sci. 2009;65:497-503. 19. Walker TS, Bais HP, Vivanco JM:Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L.(St. John's wort). Phytochemistry. 2002;60:289-293. 20.Balaji K, Veeresham C, Srisilam K, Kokate C:Azadirachtin, a novel biopesticide from cell cultures of Azadirachta indica. J Plant Biotechnol. 2003;5:121-130. 21. Muhammad R: Production of azadirachtin contents through in vitro culture of neem. University of Sindh, Jamshoro; 2012. 22. Singh M, Chaturvedi R:Statistical optimization of media for enhanced azadirachtin production from redifferentiated zygotic embryo cultures of neem (Azadirachta indica A. Juss.). In vitro Cell Dev Biol-Plant. 2012;48:92-98. 23. Srivastava S, Srivastava AK:Effect of elicitors and precursors on azadirachtin production in hairy root culture of Azadirachta indica. Appl Biochem Biotechnol. 2014;172:2286-2297. 24. Sivanandhan G, Arun M, Mayavan S, Rajesh M, Jeyaraj M, Dev GK, Manickavasagam M, Selvaraj N, Ganapathi A:Optimization of elicitation conditions with methyl jasmonate and salicylic acid to improve the productivity of withanolides in the adventitious root culture of Withania somnifera (L.) Dunal. Appl Biochem Biotechnol. 2012;168:681-696. 25. Kachroo A, Kachroo P: Salicylic acid, jasmonic acid and ethylenemediated regulation of plant defense signaling. Genet Eng: Springer; 2007:55-83. 26. Leslie CA, Romani RJ:Inhibition of ethylene biosynthesis by salicylic acid. Plant Physiol. 1988;88: 833-837. 27. Sandalio L, Dalurzo H, Gomez M, Romero‐Puertas M, Del Rio L:Cadmium‐induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot. 2001;52: 2115-2126. 28. Groppa M, Ianuzzo M, Tomaro M, Benavides M:Polyamine metabolism in sunflower plants under long-term cadmium or copper stress. Amino Acids. 2007; 32:265-275. 29. Touiserkani T, Haddad R:Cadmium-induced stress and antioxidative responses in different Brassica napus cultivars. J Agri Sci Tech. 2012;14:929-937. 30. Furze JM, Rhodes MJ, Parr AJ, Robins RJ, Withehead IM, Threlfall DR:Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Rep. 1991;10:111-114. 31. Lee K-T, Yamakawa T, Kodama T, Shimomura K:Effects of chemicals on alkaloid production by transformed roots of belladonna. Phytochemistry. 1998; 49:2343-2347. 32. Cetin ES, Babalik Z, Hallac-Turk F, Gokturk-Baydar N:The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures. Biol Res. 2014;47:1. 33. Hojati M, Modarres-Sanavy SAM, Karimi M, Ghanati F:Responses of growth and antioxidant systems in Carthamus tinctorius L. under water deficit stress. Acta Physiol Plant. 2011;33:105-112. 34. Ajungla L, Patil P, Barmukh R, Nikam T:Influence of biotic and abiotic elicitors on accumulation of hyoscyamine and scopolamine in root cultures of Datura metel L. Indian J Biotechnol. 2009;8:317-322. 35. Chavan SP, Lokhande VH, Nitnaware KM, Nikam TD:Influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of Carthamus tinctorius L. Appl Microbiol Biotechnol. 2011;89:1701-1707.
36. Mahalakshmi R, Eganathan P, Parida AK:Salicylic acid elicitation on production of secondary metabolite by cell cultures of Jatropha curcas L. Int J Pharm Sci. 2013;5:655-659. 37. Chawla H: Introduction to plant biotechnology. 3th edition ed: CRC Press Books; 2009. | ||
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