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Total Phenolic Content, Antioxidant Activity and In vitro Cytotoxicity of the Essential Oil of Jurinea leptoloba DC. | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 6، دوره 4، شماره 2، آذر 2015، صفحه 171-178 اصل مقاله (248.28 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2015.108905 | ||
| نویسنده | ||
| Mahboubeh Taherkhani* | ||
| Department of Chemistry, College of Science, Takestan Branch, Islamic Azad University, Takestan, Iran | ||
| چکیده | ||
| The essential oil from the aerial parts ofJurinea leptoloba DC., collected from Shiraz (South of Iran), was investigated for total phenolic content (TPC), antioxidant and cytotoxic effects. TPC of J. leptoloba oil was determined to be 16.53 ± 5.69 μg gallic acid equivalent/mg sample (μg GAE/mg oil) using the Folin-Ciocalteau assay. The ferric reducing power of the oil was determined 0.117 ± 0.01 GAE (mg/g).J. leptoloba oil exhibited a dose-dependent scavenging of DPPH, with an IC50 value of 24.50 mg/ml. Antioxidant activity percentage of the essential oil of J. leptoloba determined by β-carotene bleaching test revealed less potential than both standards BHT and BHA (62.01 ± 2.45%, 2.5 mg/ml oil). Cytotoxicity was measured using a modified MTT assay. IC50 valuesforHeLa and lymphocyte cells were calculated to be 290.76 and 2900.97 µg/ml, respectively. The results suggest application of J. leptoloba essentialoil as a moderate antioxidant and anticancer agent. | ||
| کلیدواژه‌ها | ||
| Jurinea leptoloba؛ Total phenolic content؛ Antioxidant activity؛ Cytotoxicity | ||
| مراجع | ||
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1. Rustaiyan A, Ganji M. Germacranolides from Jurinea eriobasis. Phytochemistry.1988;27:2991-2992.
2. Rustaiyan A, Saberi M, Habibi Z, Jakupovic J. Melampolides and other constituents from Jurinea leptoloba. Phytochemistry.1991;30:1929-1932.
3. Rustaiyan A, Habibi Z, Saberi M. Shirazolide and 14α-O-dihydroShirazolide two new elemanolides from Jurinea leptoloba.J Sci Islamic Republ Iran. 1991;2:25-27.
4. Rustaiyan A, Taherkhani M. Composition of the essential oil of Jurinea leptoloba growing wild in Iran. J. Basic. Appl Sci Res. 2013;3:708-710.
5. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem. 1999;47:3954-3962.
6. Lim TY, Lim YY, Yule CM. Evaluation of antioxidant, antibacterial and anti-tyrosinase activities of four Macaranga species. Food Chem. 2009;114:594-599.
7. Gachkar L, Yadegari, D, Bagher Rezaei M, Taghizadeh M, Alipoor Astaneh S, Rasooli I. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem. 2007;102:898-904.
8. Rasooli I, Bagher Rezaei M, Allameh A. Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock. Food Control. 2006; 17: 359-364.
9. Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. Biochemical activities of Iranian Mentha piperita L.and Myrtus communis L. essential oils. Phytochemistry. 2006;67:1249-1255.
10. Taga MS, Miller EE, Pratt DE. Chia seeds as a source of natural lipid antioxidant. J Am Oil Chemists Soc. 1984;61:928-931.
11. Sharafi SM, Rasooli I, Owlia P, Taghizadeh M, Darvish Alipoor Astaneh S. Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials. Pharmacogn Mag. 2010;6:147-153.
12. Sylvestre M, Legault J, Dufour D, Pichette A. Chemical composition and anticancer activity of leaf essential oil of Myrica gale L. Phytomedicine. 2005;12:299-304.
13. Torel J, Cillard J, Cillard P. Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry. 1986;25:383-385.
14. Ho SC, Tsai TH, Tsai PJ, Lin CC. Protective capacities of certain spices against peroxynitrite-mediated biomolecular damage. Food Chem Toxicol. 2008;46:920-928.
15. Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J Agric Food Chem. 2005;53:7749-7759.
16. Miliauskas G, van Beek TA, de Waard P, Venskutonis RP, Sudholter EJ. Identification of radical scavenging compounds in Rhaponticum carthamoides by means of LC-DAD-SPE-NMR. J Nat Prod. 2005;68:168-172.
17. Samarth RM, Panwar M, Kumar M, Kumar A. Protective effects of Mentha piperita Linn on benzo[a]pyrene-induced lung carcinogenicity and mutagenicity in Swiss albino mice. Mutagenesis.2006;21:61-66.
18. Lazutka JR, Mierauskiene J, Slapsyte G, Dedonyte V. Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthax piperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001;39:485-492. | ||
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