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Autecology, Ethnopharmacology, Phytochemical, Antioxidant and Antimicrobial Activity of Thymus carmanicus Jalas. from Golestan Province in North of Iran | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 10، دوره 4، شماره 1، تیر 2015، صفحه 67-75 اصل مقاله (202.62 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2015.108893 | ||
| نویسنده | ||
| Masoumeh Mazandarani* | ||
| Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran | ||
| چکیده | ||
| This research had been carried out to ecological characters, phytochemical, anti bacterial and antioxidant activity of Thymus carmanicus Jalas. in Golestan province .In several field observation, the aerial parts of plant in blooming were collected from Deraznoo Mountain inSeptember 2013 (2700 m). Essential oil was obtained by hydro distillation and analyzed by GC/MS. Phytochemical assay included: TP (total phenol) and TF (Total flavonoid) were determined by spectrophotometrically, antioxidant capacity were obtained by TAC, RP and DPPH methods and the antibacterial by using agar well method and the minimum inhibitory concentration (MIC) assay. Results showed that T. carmanicus is the most aromatic mountainous herb, which wild growing in cold dry climate (1800-2750 m) in Golestan province, with annual raining 30.5 mm, annual temperature 17.5 °C , in sandy clay loam soil with pH= 7.5 and Ec=2.1 dc and have long been used in traditional medicines of this region as a strong tonic, anti inflammation, carminative, anti virus, anti infection, anti fungus, anti ulcer and sedative . The carvacrol (41.4%), thymol (27.2%) and β-caryophylene were the major constituents of T. carmanicus oil , with total phenols (TP= 81.7±0.3 GAE/ g), total flavonoids (TF= 34.2±0.8 QUE/g), which had good antioxidant activity (IC50 = 21.8±0.1 µg/ml ), especially in DPPH method .The maximum activity of the essential oil was observed against Candida albicans ( 35.8±0.6 mm), S.epidermidis ( 33.1± 0.4 mm), B. subtilus ( 29.5± 0.2 mm), S.aureus ( 28.3±0.2 mm), E.coli (23.1±0.4 mm), Enterococcus faecalis ( 17.2±0.8 mm) and Kelebsiella pneumoniae ( 16.5±0.2mm) with MIC value in the range of 10 - 132 mg/mL. According to these results, the aerial parts of plant have rich source of terpenoides , flavonoid and phenolic componds and showed good antioxidant and antibacterial activity, which will be confirmed the traditional uses of T. carmanicus Jalas. in these regions as an good anti-inflammatory, sedative and anti infection. | ||
| کلیدواژه‌ها | ||
| Antioxidant؛ Autecology؛ essential oil؛ Ethnopharmacology؛ Golestan Province؛ TF and TP content؛ Golestan؛ Thymus carmanicus Jalas | ||
| مراجع | ||
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1. Mazandarani M, Borhani G, Fathiazad F. Phytochemical analysis, antioxidant activity and ecological requirements of Capparis spinosa L. in Golestan and Semnan provinces (North of Iran). J. Med. Plants By-prod. 2014; 1: 21-26. 2. Zarghami Moghaddam P, Mazandarani M, Zolfaghari MR . Antibacterial and antioxidant activities of root extract of Onosma dichroanthum Boiss. in north of Iran. Afr. J. Microbiol. Res. 2012;6:1776-1781. 3. Zargari A. Medicinal Plants. Tehran, Iran: Tehran University Press, 1990;4:2842 4. Amin G. Popular medicinal plants of Iran. Tehran, Iran: Tehran University of Medical Sciences Press. 2005. 5. Nejad Ebrahimi S, Hadian b, Mirjalili MH, Sonboli C, Yousefzadie D. Essential oil composition and antibacterial activity of Thymus caramanicus at different phenological stages. Food Chem 110 .2008;927-931. 6. Sfaei-Ghomi J, Meshkat alsadat MH, Shamaei SH, Hashem Nejad M, Hasani A. Chemical charachtirization of bioactive volatile molecoules of four Thymus species using nanoskale injection method. Digest J. Nanomater. Biostruct. 2009; 4:835-841. 7. Hajimehdipoor H, Khanavi M, Zahedi H, Abedi Z, Kalantari Khandani N, Adib N, Pirali Hamedani M . Fingerprint Study of Thymus spp. by TLC. 2009;31:19-24. 8. Zenasni L, Bakhy KH, Gaboun F, Mousadak R, Benjouad AA, Al Faiz SH. Essential oil composition and biomass productivity of Moroccan endemic Thymus satureioides Coss. & Ball. growing in the Agoundis Valley . J. Med. Plant Res. 2014;8: 504-512. 9. Karaman S, Digrak M, Ravid U, Ilcim A. Antibacterial and antifungal activity of the essential oils of Thymus revolutus Celak. from Turkey. J. Ethnopharmacol. 2001;76:183-186. 10. Rasooli I, Mirmostafa S A. Antibacterial properties of Thymus pubescens and Thymus serpyllum essential oils. Fitoterapia. 2002;73:244-250. 11. Rasooli I, Mirmostafa SA. Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus. J Agric Food Chem. 2003;51:2200-2205. 12. Miguel G, Simoes M, Figueiredo AC, Barroso J.G, Pedro LG, Carvalho L . Composition and antioxidant activities of the essential oils of Thymus caespititius, Thymus camphoratus and Thymus mastichina. Food Chemistry.2004;86:183-188. 13. Pourmorad F, Hosseinimehr SJ, Shahabimajd N . Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol. 2006;5:1142-1145. 14. Arabshahi-Delou S, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 2007;102:1233-40. 15. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J. Appl. Microbiol.1999; 86:985-990. 16. Imelouane B, Amhamdi H, Wathelet JP, Ankit M, Khedid K, Elbachiri A. Chemical Composition and Antimicrobial Activity of Essential Oil of Thyme (Thymus vulgaris) from Eastern Morocco .Int. J. Agric. Biol. 2009;11:205-208. 17. Mazandarani, M, Osia N, Ghafourian M. Antioxidant activity and ethnopharmacological survey of Achillea biebersteinii Afan. in treatment of Dysmenorrhoea in traditional medicine of Golestan Province, Iran. Int. J. Women’s Health Reprod. Sci. 2015; 3:107-110. 18. Mazandarani M, Mirdeilami S Z, Pessarakli M. Essential oil composition and antibacterial activity of Achillea millefolium L. from different regions in North east of Iran. J. Med. Plants Res.2013; 7:1063-1069. 19. Ogbulie JN, Ogueke CC, Okoli IC, Anyanwu BN Antibacterial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. Afr. J. Biotechnol. 2007;6:1544-1548. 20. Mojab F, Nickavar B. Composition of the essential oils from three species from Labiatae from Iran. Planta Medica. 2006:72. 21. Rustaiyan A, Masoudi SH, Monfared A, Kamalinejad M, Lajevardi T, Sedaghat S. Volatile constituents of three Thymus species grown wild in Iran.Planta Medica. 2000;66:197-198. 22. Sefidkon F, Dabiri M, Mirmostafa S A. The essential oil of Thymus persicus (Ronniger ex Rech. f.) Jalas from Iran. J. Essent. Oil Res. 2002b;14:351-352. 23. Sadjadi S E, Khatamsaz M . Composition of the essential oil of Thymus daenensis Celak. ssp. lancifolius (Celak.) Jalas. J. Essent. Oil Res. 2003;15:34-35. 24. Knowles J R, Roller S, Murray DB, Naidu A S. Antimicrobial action of carvacrol at different stages of dual-species biofilm development by Staphylococcus aureus and Salmonella entericsero var. typhimurium. Appl. Environ. Microbiol.2005;71:797-803. 25. Kessler M, Ubeaud G, Jung L. Antiand pro‐oxidant activity of rutin and quercetin derivatives.J. Pharma. Pharmacol. 2003;55:131‐142 26. Zheng W, Wang SY. Antioxidant Activity and Phenolic Compounds in Selected Herbs. J. Agric. Food Chem. 2001;49:5165-5170. 27. Mozaffarian V. A dictionary of iranian plant names. 1996; N(5558):400. | ||
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