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In vitro Valuation of Toxic Effects of Fennel (Foeniculum vulgare Miller.) Seed Decoctions on Plant and Human Cells | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 9، دوره 1، شماره 2، آذر 2012، صفحه 145-150 اصل مقاله (1022.78 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2012.108478 | ||
| نویسندگان | ||
| Samirasadat Mirfendereski1؛ Mehrnaz Keyhanfar* 2؛ Khosro Piri1 | ||
| 1Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran | ||
| چکیده | ||
| The medicinal plants have been used since ancient times and are still utilized by the majority of the population. Hence, it is essential to investigate the cytotoxic and genotoxic effects of these plants. The aim of this study was to evaluate the toxic effects of fennel seeds decoctions, traditionally used in Iran as an herbal remedy. The cytotoxicity was tested in vitro using Allium cepa L. roots and human cells and genotoxicity were evaluated using A. cepa L. roots. The seeds decoctions were prepared in the traditional method and in two concentrations, commonly used in Iran (CC) and 10 times concentration (10C). The A. cepa L. root tips were treated with the decoctions and the mitotic index (MI) and chromosome aberrations were assessed. Human lymphocytes and human bone marrow endothelial cell line (HBMEC) were also treated with the extracts and the cell viabilities were measured using trypan blue and lactate dehydrogenase (LDH) assays, respectively. Although both of the extracts decreased MI in the A. cepa L. root tip cells, only the 10C extract significantly increased chromosomal aberrations. In addition, dilutions 1:30, 1:62.5, 1:125 and 1:250 from the 10C extract were 100% cytotoxic to the human lymphocyte cells, however for the CC extract, only dilution 1:30 showed cytotoxic effects. The 1:30 dilution of the 10C extract caused 65% toxicity in HBMEC and none of the CC extract dilutions were toxic to these cells. The tested decoctions showed some cytotoxic and genotoxic effects and the safe dosage of the traditional decoctions needs to be precisely evaluated. | ||
| کلیدواژه‌ها | ||
| Foeniculum vulgare Miller؛ Allium cepa L؛ Cytotoxic؛ Genotoxic | ||
| مراجع | ||
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1. Asghari G. Biotechnology of medicinal plants and herbal medicines production Jahad Daneshgahi Isfahan. 2006.
2. Fu PP, Chiang HM, Xia Q, Chen T, Chen BH, Yin JJ, Wen KC, Lin G, Yu H. Quality assurance and safety of herbal dietary supplements. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009;27:91-119.
3. Zargari A. Medicinal plants. Tehran University, 1991.
4. Ruberto G, Baratta M T, Deans SG, Dorman HJ. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimumessential oils. Planta Med. 2000;66:687-693.
5. Ghassemi Dehkordi N, and colleagues. Iranian herbal pharmacopoeia. Ministry of Health and Medical Education. 2002.
6. Omidbaigi R. Approaches to production and processing of medicinal plants. Tarrahan-e-nashr. 2000.
7. Ozcan M, Chalchat JC, Arslan D, Ates A, Unver A. Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare subsp. piperitum) fruit oils obtained during different vegetation. J Med Food. 2006;9:552-561.
8. Sing G, Maurya S, De Lampasona MP, Catalan C. Chemical constituents, antifungal and antioxidative potential of Foeniculum vulgare volatile oil and its acetone extract. Food Control 2006;17:745-752.
9. Babatunde BB, Bakare AA. Genotoxicity Screening of Wastewaters from Agbara Industrial Estate, Nigeria Evaluated with the Allium Test. Pollution Research. 2006;25:227-234.
10. Bakare AA, Wale-Adeyemo AR. The mutagenic and cytotoxic effects of leachates from domestic solid wastes and Aba-Eku landfill, Nigeria on Allium cepa L. Nat Environ Poll Technol. 2004;3:455-462.
11. Fiskesjo G, Allium test for screening chemicals; evaluation of cytological parameters. Plants for Environmental Studies CRC Press. 1997;307-333.
12. Kwon Y, Choi W, Kim WC, Kim W, Kim M., Kang W, Kim C. Antimicrobial constituents of Foeniculum vulgare. Arch Pharm Res. 2002;25:154.
13. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DE, Tice R, Waters MD, Aitio A. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutat Res. 2000; 463:111-172.
14. Krishna G, Hayashi M. In vivo rodent micronucleus assay: protocol, conduct and data interpretation. Mutat Res. 2000;455:155-166.
15. Askin Celik T, Aslanturk OS. Evaluation of cytotoxicity and genotoxicity of Inula viscosa leaf extracts with Allium test. J Biomed Biotechnol. 2010; 2010: 189252. doi: 10.1155/2010/189252
16. Palanikumar L, Ragunathan I, Panneerselvam N. Chromosome aberrations induced by curcumin and aloin in Allium cepa L. root meristem cells. Turkish J Biol. 2011;35:145-152.
17. Gorelick NJ. Genotoxicity of trans-anethole in vitro. Mutat Res. 1995;326:199-209.
18. Abraham SK. Anti-genotoxicity of trans-anethole and eugenol in mice. Food Chem Toxicol. 2001;39:493-498.
19. Ebeed NM, Abdou HS, Booles HF, Salah SH, Ahmed ES, Fahmy K. Antimutagenic and Chemoprevention Potentialities of Sweet Fennel (Foeniculum vulgare Mill.) Hot Water Crude Extract. J Am Sci. 2010;6:831-842. | ||
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