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مطالعه‌ی اثر بیوکنترلی سویههای سودوموناس فلورسنت مولد 2 و 4- دی استیل فلوروگلوسینول بر نماتد ریشه گرهی در ریزوسفر ارقام گوجه‌فرنگی | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 3، دوره 4، شماره 1 - شماره پیاپی 7، شهریور 1395، صفحه 27-39 اصل مقاله (313.48 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2016.108153 | ||
| نویسندگان | ||
| سعیده تنها1؛ فرشته بیات* 2؛ فاطمه جمالی3؛ آیت اله سعیدی زاده4 | ||
| 1دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر | ||
| 2دانشگاه خلیج فارس | ||
| 3گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی دانشگاه شاهد تهران | ||
| 4دانشگاه شاهد | ||
| چکیده | ||
| نماتد مولد ریشه گرهی Meloidogyne javanica یکی از عوامل مهم بیمارگر گوجه‌فرنگی در کشور به‌شمار میرود. در این تحقیق کنترل بیولوژیک این نماتد توسط سویههای UTPF68 و UTPF101باکتری Pseudomonas fluorescens، مورد ارزیابی قرار گرفت. ابتدا سویهها از نظر دارا بودن ژن phlD از طریق واکنش زنجیرهای پلیمراز (PCR) مورد بررسی قرار گرفتند. سپس توانایی تولید متابولیتهای ضد میکروبی توسط این باکتریها و تأثیر آن‌ها بر تفریخ تخم و فعالیت لارو سن دوم نماتد در شرایط آزمایشگاه و اثر بیوکنترلی آن‌ها بر فعالیت نماتد در ریزوسفر چهار رقم گوجه‌فرنگی در شرایط گلخانه براساس طرح بلوکهای کامل تصادفی با چهار تکرار مورد بررسی قرار گرفت. نتایج واکنش زنجیرهای پلیمراز نشان داد که هر دو سویه واجد ژن phlD بوده و قطعه DNA با طول 629 جفت باز در آن‌ها تکثیر شد. سویههای باکتریایی قادر به تولید آنتی بیوتیک DAPG، سیانید هیدروژن و پروتئاز در شرایط آزمایشگاهی بودند. سویه UTPF68 نسبت به سویه UTPF101 توانایی بیشتری در عدم تفریخ تخم نماتد داشت، درحالی‌که میزان مرگ و میر لارو سن دوم در هر دو سویه یکسان بوده است. نتایج گلخانه‌ای نشان داد که سویههای باکتری موجب افزایش شاخصهای رشدی ارقام و کاهش فعالیت نماتد در ریزوسفر آن‌ها شدند. میزان افزایش رشد یا کنترل نماتد بسته به رقم گوجه‌فرنگی و سویه باکتری متفاوت بود. | ||
| کلیدواژه‌ها | ||
| گوجه‌فرنگی؛ نماتد ریشه گرهی؛ سودومونادهای فلورسنت؛ 2 و 4- دی استیل فلوروگلوسینول | ||
| عنوان مقاله [English] | ||
| Evaluation of Pseudomonas fluorescens strains for biological control of root-knot nematode in some tomato cultivars | ||
| نویسندگان [English] | ||
| Saeedeh Tanha1؛ FERESHTEH BAYAT2؛ Fatemeh Jamali3؛ Ayatollah Saeedizadeh4 | ||
| 2PERSIAN GULF UNIVERSITY | ||
| 4Shahed University | ||
| چکیده [English] | ||
| The root-knot nematode, Meloidogyne javanica, is one of the major tomato pathogens in Iran. In the current study, Pseudomonas fluorescens UTPF101 and UTPF68 strains were evaluated for biological control of the pathogen. The strains were studied in terms of having phlD gene through polymerase chain reaction (PCR). The ability of bacteria to produce antimicrobial metabolites and their effect on hatching eggs and larvicidal activity were then studied in vitro. Finally, the ability of bacteria to control nematode on four varieties of tomatoes was studied via a factorial experiment in a randomized complete block design with four replications under greenhouse conditions. PCR procedure results showed that both strains contained phlD gene and 629 bp DNA fragment was reproduced in both of them. Bacterial strains were capable of producing DAPG antibiotic, hydrogen cyanide and protease in in vitro conditions. UTPF68 strain showed a greater impact on the egg hatching compared to strain UTPF101, while in terms of the impact on larvae mortality; both strains performed the same. The greenhouse results indicated that both bacterial strains increased the growth factors and reduced nematode pathogenicity in the infected plants. The rate of growth or controlling nematode was different depending on tomato cultivar or bacterial strain. | ||
| کلیدواژه‌ها [English] | ||
| Fluorescent pseudomonads, Root-knot nematode, tomato, 2-4-diacetylphloroglucinol | ||
| مراجع | ||
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