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کنترل بیولوژیک بیماری پژمردگی ورتیسیلیومی سیب زمینی با استفاده از عوامل بیوکنترل انتخابی (مقالهی انگلیسی) | ||
| آفات و بیماری های گیاهی | ||
| مقاله 16، دوره 84، شماره 2 - شماره پیاپی 103، اسفند 1395، صفحه 11-20 اصل مقاله (282.87 K) | ||
| نوع مقاله: مدیریت آفات و بیماریهای گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2017.108122 | ||
| نویسنده | ||
| جهانشیر امینی* | ||
| دانشیار گروه گیاهپزشکی دانشکده کشاورزی، دانشگاه کردستان | ||
| چکیده | ||
| بیماری پژمردگی ورتیسیلیومی سیب زمینی از عوامل محدود کننده کشت و تولید سیب زمینی است. در این تحقیق 14 عامل کنترل کننده زیستی از هفت گونه متفاوت شامل Trichoderma harzianum، Trichoderma deliquescens، Fusarium oxysporum، Talaromyces flavus، Bacillus subtilis، Pseudomonas fluorescens و Serratia marcescens به منظور بررسی فعالیت ضد قارچی در شرایط آزمایشگاه و گلخانه بر علیه قارچ بیمارگر Verticillium dahliae مورد آزمایش قرار گرفت. مکانیسم تاثیر آنتاگونیستی عوامل بیوکنترل علیه قارچ بیمارگر به روشهای کشت متقابل، تولید مواد فرار و مایع خارج سلولی بررسی گردید. تمام عوامل بیوکنترل سبب کاهش قابل توجهی از رشد پرگنه قارچ بیمارگر در شرایط آزمایشگاه شدند. جدایه های T. flavus بیشترین تاثیر بازدارندگی (7/66 – 4/65 درصد) به روش کشت متقابل روی قارچ بیمارگر ایجاد کردند. توانایی عوامل بیوکنترل در تولید سیدروفور، پروتئاز، سیانید هیدروژن و اندول استیک اسید نیز متفاوت بود. نتایج آزمایشات گلخانه ای بعد از 75 روز نشان داد تمام عوامل بیوکنترل سبب کاهش شدت بیماری و تغییر رنگ آوند در گیاهان تیمار شده به میزان متفاوتی شدند. بر این اساس، جدایه T. flavus TFPV24 بیشترین تاثیر را در کاهش شدت بیماری (76%) و تغییر رنگ آوند داشت و باعث افزایش محصول (171%) در مقایسه با شاهد گردید. بطور کلی جدایه های T. flavus در شرایط آزمایشگاه و گلخانه بیشترین و باکتری S. marcescens SMTR کمترین تاثیر آنتاگونیستی را روی کنترل قارچ بیمارگر داشتند. بنابراین، نتایج این تحقیق نشان داد جدایه هایT. flavus توانایی کنترل زیستی بیماری پژمردگی ورتیسیلیومی سیب زمینی را دارند. | ||
| کلیدواژهها | ||
| کنترل بیولوژیک؛ پژمردگی سیب زمینی؛ Talaromyces flavus؛ Fusarium oxysporum | ||
| عنوان مقاله [English] | ||
| Biocontrol of Verticillium wilt of potato caused by Verticillium dahliae using selected biocontrol agents (Article in English) | ||
| نویسندگان [English] | ||
| J. AMINI | ||
| چکیده [English] | ||
| Verticillium wilt of potato is a major limiting factor in potato production. In this study, a total of 14 biocontrol agents, consisting of seven different species (Trichoderma harzianum, Trichoderma deliquescens, Fusarium oxysporum, Talaromyces flavus, Bacillus subtilis, Pseudomonas fluorescens and Serratia marcescens), were examined for antifungal activity in vitro and greenhouse conditions against Verticillium dahliae which cause potato wilt disease. Antagonistic effects of biocontrol agents were evaluated against V. dahliae, through volatile and non-volatile metabolite production mechanisms in in vitro. All antagonists caused significant reduction in growth of V. dahliae compared to control. The maximum antagonistic properties were observed in isolates of T. flavus with inhibition zones ranging from 65.4 to 66.7% by dual culture method. The ability of biocontrol agents were varied in production of siderophore, protease, cyanide hydrogen and indole acetic acid. Results of the greenhouse study in 75 days after sowing indicated that all biocontrol agents reduced disease severity and vascular discoloration of Verticillium wilt at different rates. Accordingly, Trichoderma flavus TFPV24 was the most effective in reducing disease severity and vascular discoloration of potato Verticillium wilt by 76% and increasing yield by 171% compared to the untreated control. In general, in both in vitro and greenhouse assay, isolates of T. flavus were the most effective antagonist and the least antagonistic effect belonged to Serratia marcescens. The overall results of this study showed that isolates of T. flavus have high efficacy in controlling potato Verticillium wilt. | ||
| کلیدواژهها [English] | ||
| biocontrol, Verticillium wilt, Talaromyces flavus, Fusarium oxysporum | ||
| مراجع | ||
|
ABO-ELYOUSR, K. A. and M. M. HASHEM, 2009. Biological control of Fusarium wilt in tomato by plant growth-promoting yeasts and rhizobacteria. Plant Pathology Journal, 25(2): 199-204.
AHMADZADEH, M. and A. SHARIFI TEHRANI, 2009. Evaluation of fluorescent Pseudomonads for plant growth promotion, antifungal activity against Rhizoctonia solani on common bean, and biocontrol potential. Biological Control, 48: 101-107.
ALSTROM, S. 1987. Factors associated with detrimental effects of rhizobacteria on plant growth. Plant soil, 102: 3-9.
AMINAE, M. M., B.MANSOORI and D. ERSHAD, 2006. A study on Verticillium wilt of Potato in Kerman province. In Proceedings of the 17th Iranian plant protection Congress, 163 University of Tehran, Karaj.
AMINI, J. and F. DZHALILOV, 2010. Induction of systemic resistance in tomato plants to Fusarium oxysporum f. sp. lycopersici causal agent of Fusarium wilt of tomato by non-pathogenic F. oxysporum under greenhouse conditions. Applied Entomology and Phytopathology, 78(1): 15-32.
AYER, W. A. and J. S. RACOK, 1990. The metabolites of Talaromyces flavus. Part 1. Metabolites of organic extract. Canadian Journal of Chemistry, 68: 2085-2094.
BAE, Y. S. and G. R. KNUDSEN, 2007. Effect of sclerotial distribution pattern of Sclerotinia sclerotiorum on biocontrol efficacy of Trichoderma harzianum. Applied Soil Ecology, 35(1): 21-24.
BILODEAU, G. J., S. T. KOLIKE, URIBE, P. and F. N. MARTIN, 2012. Develiopment of an assay for rapid deterction and quantification of Verticillium dahlia in soil. Phytopathology, 102: 331-343.
BOHUMIL PROKSA. 2010. Talaromyces flavus and its metabolites. Chemical Papers, 64(6): 696-714.
BRIC, J. M., R. M. BOSROCK and S. E. SILVERSONE, 1991. Rapid in situ assay for indole acetic acid production by bacteria immobilization on a nitrocellulose membrane. Applied and Environmental Microbiology, 57: 535-538.
CHANTAWANNAKUL, P., A. ONCHAROEN, K. KLANBUT, E. CHUKEATIROTE and S. LUMYONG, 2002. Characterization of proteases of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in northern Thailand. Science Asia, 28: 241-245.
DESJARDINS, A. E., S. P. MCCORMICK and D. L. CORSINI, 1995. Diversity of sesquiterpenes in 46 potato cultivars and breeding selections. Journal of Agricultural and Food Chemistery, 43: 2267-2272.
DUO-CHUAN, L. I., S. CHEN and L. U. JINA, 2005. Purification and partial characterization of two chitinases from the mycoparasitic fungus Talaromyces flavus. Mycopathologia, 159: 223-229.
EL-TARABILY, K. A., M. H. SOLIMAN, A. H. NASSER, H. A. AL-HASSANI, K. SIVASITHAMPARAM, F. MCKENNA and G. S. HARDY, 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathololgy, 49(5): 573-583.
ERDOGAN, O. and K. BENLIOGLU, 2010. Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. Under field conditions. Biological Control, 53: 39-45.
FAHIMA, T. and Y. HENIS, 1995. Quantitative assessment of the interaction between the antagonistic fungus Talaromyces flavus and the wilt pathogen Verticillium dahliae on eggplant roots. Plant soil, 176(1): 129-137.
FAHIMA, T., Y. HENIS and D. HORNBY, 1990. Interactions between pathogen, host and biocontrol agent: multiplication of Trichoderma hamatum and Talaromyces flavus on roots of diseased and healthy hosts. In Biological control of soil-borne plant pathogens, 165-180: CAB International.
FAHIMA, T., L. MADI and Y. HENIS, 1992. Ultrastructure and germinability of Verticillium dahliae microsclerotia parasitized by Talaromyces flavus on agar medium and in treated soil. Biocontrol Science and Technology, 2: 69-78.
IAKOVOS, S. P., E. T. SOTIRIOS, A. S. IOANNIS, C. IORDANIS and J. P. EPAMINONDAS, 2009. Mode of action of a non-pathogenic Fusarium oxysporum strain against Verticillium dahliae using real time QPCR analysis and biomarker transformation. Biological Control, 50(1): 30-36.
ISSAC, I. 1967. Speciation in Verticillium. Annu Rev Phytopathol, 5(1): 201-222.
JOHNSON, D. A., R. BAKER and R. A. BOYDSTON, 2013. Field evaluation of mutant and hybrid lines of mint for resistance to Verticillium wilt and yield. Crop protection, 43: 1-6.
KARIMI, K., J. AMINI, B. HARIGHI and B. BAHRAMNEJAD, 2012. Evaluation of biocontrol potential of pseudomonas and bacillus spp. against Fusarium wilt of chickpea. Aust J Crop Science, 6(4): 695-703.
KAZEMPOUR, M. N. 2004. Biological control of Rhizoctonia solani, the causal agent of rice sheath blight by antagonistic bacteria in greenhouse and field conditions. Plant Pathology journal, 3(2): 88-96.
KIM, K. K. A., D. R. FRAVEL and G. C. PAPAVIZAS, 1990. Glucose oxidas as the antifungal principle of talaron from Talaromyces flavus. Canadian Journal of Microbiology, 36(11): 760-764.
KOTAN, R., S. FIKRETTIN, D. ERKOL and E. CAFER, 2009a. Biological control of the potato dry rot caused by Fusarium species using PGPR strains. Biological Control, 50(2): 194-198.
KOTAN, R., F. SAHIN, E. DEMIRIC and C. EKEN, 2009b. Studies on the biological control of potato dry rot disease caused by Fusarium solani with application of some bacterial strains. In 5th Biological control congress Erzuum, Turkey.
KRAUS, J. and J. E. LOPER, 1990. Biocontrol of Pythium damping-off of cucumber by Pseudomonas fluorescens pf-5: Mechanistic studies In Plant Growth Promoting Rhizobacter, 172-175 (Eds C. Keel, B. Koller and G. Defago). Interlaken, Switzerland: The second international workshop on plant growth promoting rhizobacteria
LETICIA, Q., A. GASTON, B. NATALIA, V. PATRICIA, P. CARLOS, D. FERNANDO, C. MONICA, A. NORA and A. ALICIA, 2009. Three native Pseudomon fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa. Biological Control, 51: 42-50.
LOLIAM, B., T. MORINAGA and S. CHAIYANA, 2013. Biocontrol of Pythium aphanidermatum by the cellulolytic actinomycetes Streptomyces rubrolavendulae S4. Science Asia, 39(6): 584-590.
MADI, L., T. KATAN, J. KATAN and Y. HENIS, 1997. Biological control of Sclerotinia rolfsii and Verticillium dahlia by Talaromyces flavus is mediated by different mechanisms. Phytopathology, 87: 1054-1060.
MAROIS, J. J., D. R. FRAVEL and G. C. PAPAVIZAS, 1984. Ability of Talaromyces flavus to occupy the rhizosphere and its interaction with Verticillium dahliae. Soil Biology and Biochemistry, 6: 387-390.
MENENDEZ, A. B. and A. GODEAS, 1998. Biological control of Sclerotinia sclerotinia attacking soybean plants: degradation of the cell wall of this pathogen by Trichoderma harzianum. Mycopathology, 142: 153-160.
NARAGHI, L., A. HEYDARI, S. REZAEE, M. RAZAVI, and H. JAHANFAR, 2010. Study on antagonistic effect of Talaromyces flavus on Verticillium albo-atrum, the causal agent of potato wilt disease. Crop protection, 29: 658-662.
OLESEN, M. H., L. C. DELEURAN, R. GISLUM and B. BOELT, 2014. Preventing an increase in Verticillium wilt incidence in spinach seed production. Crop protection, 66: 107-113.
PROKSA, B., J. ADAMCOVA and J. FUSKA, 1992. 2-Methylsorbic acide, an antifungal metabolite of Penicillium vermiculatum. Journal Applied Microbiology and Biotechnology, 37: 443-445.
SADEGHI, A., E. KARIMI, P. A. DAHAJI, M. G. JAVID, Y. DALVAND and H. ASKARI, 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology, 28(4): 1503-1509.
SAEED, R., N. KAKVAN, A. HEYDARI, L. NARAGHI, and H. R. ZAMANIZADEH, 2013. Development of new bioformulation using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping-off disease. Crop protection, 53: 80-84.
SPINK, D. S. and R. C. ROWS, 1989. Evaluation of Talaromyces flavus as abiological control agent against Verticillium dahliae in potato. Plant Disease, 73: 230-236.
SRIVIDYA, S., A. THAPA, D. V. BHAT, K. GOLMEI and N. DEY, 2012. Streptomyces sp. 9p as effective biocontrol against chilli soilborne fungal phytopathogens. Eurropean Journal of Experimental Biology, 2(1): 163-173.
TABARRAEI, M., J. AMINI and B. HARIGHI, 2011. Effects of fluorescent Pseudomonads for control of damping-off disease of cantaloupe caused by Phytophthora drechsleri. Australian Journal Crop Science, 5(11): 1427-1433.
TANAKA, Y. and S. OMURA, 1993. Agroactive compounds of microbial origin. Annual Review of Microbiology, 47(1): 57-87.
TARIQ, M., S. YASMIN and F. Y. HAFEEZ, 2010. Biological control of potato black scurf by rhizosphere associated bacteria. Brazilian Journal of Microbiology, 41(2): 439-451.
THANASSOULOPOULOS, C. C. and W. J. HOOKER, 1968. Factors influencing infection of field grown potato by Verticillium albo-atrum. American Potato Journal, 45: 203-216.
TJAMOS, E. C. and D. R. FRAVEL, 1995. Detrimental effect of sublethal heating and Talaromyces flavus on microsclerotial of Verticillium dahlia. Phytopathology, 85: 388-392.
TJAMOS, E. C. and D. R. FRAVEL, 1997. Distribution and establishment of the biocontrol fungus Talaromyces flavus in soil and on roots of solanaceous crops. Crop protection, 16(2): 135-139.
UPPAL, A, K. and A. E. HADRAMI, R. L. ADAM, M. TENUTA and F. DAAY, 2008. Biological control of potato Verticillium wilt under controlled and field conditions using selected bacterial antagonists and plant extracts. Biological Control, 44: 90-100.
WELLER, D. M. and R. J. COOK, 1983. Suppression of take all of wheat by seed treatment with fluorescent pseudomonads. Phytopathology, 73: 463-469.
YANG, P., Z. X. SUN, S. Y. LIU, H. X. LU, Y. ZHOU and M. SUN, 2013. Combining antagonistic endophytic bacteria in different growth stages of cotton for control of Verticillium wilt. Crop prottection, 47: 17-23.
YANGUI, T., S. SAYADI, A. GARGOUBI and A. DHOUIB, 2010. Fungicidal effect of hydroxytyrosol-rich preparation from olive mill wastewater against Verticillium dahlia. Crop protection, 29: 1208-1213. | ||
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