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اثر عصاره برخی گیاهان دارویی، اسید آلی و آنتیبیوتیک بر کاهش کلنیزاسیون باکتری کمپیلو باکتر ژژونی در روده جوجههای گوشتی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 32، شماره 5 - شماره پیاپی 79، دی 1395، صفحه 795-806 اصل مقاله (236.58 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.107996 | ||
| نویسندگان | ||
| کریم چعب1؛ شعبان رحیمی* 2؛ پژواک خاکی3 | ||
| 1دانشآموخته کارشناسیارشد، گروه علوم طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2استاد، گروه علوم طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3استادیار، گروه میکروبیولوژی، مؤسسه تحقیقات واکسن و سرمسازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| این آزمایش بهمنظور بررسی اثر گیاهان دارویی، اسید آلی و آنتیبیوتیک بر عملکرد جوجههای گوشتی انجام شد. گروههای آزمایشی شامل: گروه اول کنترل منفی (غیرآلوده)، گروه دوم کنترل مثبت (آلوده)، گروههای سوم، چهارم و پنجم بهترتیب شامل عصارههای گیاهی آویشن، سیر و بومادران (هر یک به میزان 1/0% در طول دوره)، گروه ششم اسید آلی (Selko-pH به میزان 1/0%، در دو هفته اول بهصورت 24 ساعت و در ادامه دوره 8-7 ساعت در روز) در آب آشامیدنی و گروه هفتم آنتیبیوتیک (اکسی تتراسایکلین به میزان 100 گرم در تن) بودند. در روز 21 تمام گروهها بجز کنترل منفی با باکتری کمپیلو باکتر ژژونی چالش (1 میلیلیتر) داده شدند. نتایج در 42 روزگی نشان داد که بالاترین عیار آنتیبادی علیه گلبولهای قرمز خون گوسفند مربوط به تیمار اسید آلی بود که نسبت به تیمار شاهد تفاوت معنیداری داشت (P<0.05). البته تیمارها بر روی ترکیبهای بیوشیمیایی خون تأثیر معنیداری گذاشتند (P<0.05)، بهطوری که تیمار آویشن باعث کاهش کلسترول سرم و تیمار سیر نیز کمترین میزان تریگلیسرید را نشان داد که از لحاظ آماری با تیمار شاهد تفاوت معنیداری داشتند (P<0.05). تیمارها تأثیر معنیداری بر مورفولوژی روده داشتند، بهطوری که بالاترین ارتفاع پرز و بیشترین عمق کریپتها بهترتیب در تیمارهای سیر و آویشن مشاهده شد که از لحاظ آماری با تیمار شاهد تفاوت معنیداری داشت (P<0.05). همچنین تیمارها بر موضعی شدن باکتری کمپیلو باکتر ژژونی تأثیر معنیداری داشتند (P<0.05)، بهطوری که کمترین جمعیت کلونی این باکتری در تیمار آنتیبیوتیک مشاهده شد و تیمار بومادران نیز باکتری را نسبت به تیمار شاهد بهصورت معنیداری کاهش داد. | ||
| کلیدواژهها | ||
| چالش؛ آویشن؛ بومادران؛ سیر؛ مورفولوژی روده؛ جوجه گوشتی | ||
| عنوان مقاله [English] | ||
| Effects of herbal extract, organic acid and antibiotic on reducing colonization of Campylobacter jejuni in intestine of broiler chickens | ||
| نویسندگان [English] | ||
| K. Chaab1؛ Sh. Rahimi2؛ P. Khaki3 | ||
| 1M.Sc. Graduate, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| This study was conducted to compare three herbal extracts (thyme, yarrow and garlic), antibiotic, and organic acids to reduce Campylobacter jejuni colonization in digestive tract of broiler chickens. A total of 336 one-day old male broilers (Cobb 500) were randomly allocated to 7 treatments, 4 replicates with 12 birds in each replicate with a completely randomized design. The experimental groups included negative control (basal diet without challenge), the positive control (basal diet with challenge), organic acids (1 mL per liter in drinking water for first 14 days of age, then 8 hours each day until end of the experiment), antibiotic (oxytetracycline 100 ppm in feed), yarrow extract (1 mL per liter in drinking water), thyme extract (1 mL per liter in drinking water), and garlic extract (1 mL per liter in drinking water) throughout the experiment. Except for the negative control group, all chickens were orally challenged with (109cfu/mL) C. jejuni suspension on day 21. The negative control group was inoculated with 1 mL of saline solution. Feed intake, weight gain, and feed conversion ratio were measured weekly. At end of the experiment (day 42), the blood samples were taken from the wing vein to determine serum biochemical parameters. The effect of treatments on the morphology of the small intestine, lymphatic organs weight, and colonization of C. jejuni were measured. The results of statistical analysis showed that treatments had significant effect (P>0.05) on Campylobacter jejuni colonization,so that, the highest and lowest levels of the bacterial colony were observed in positive control and antibiotic treatment. The negative control and yarrow treatments had a significant difference (P<0.05) with positive control. The experimental groups showed a significant effect on intestinal morphology and blood parameters (P>0.05). According to the results of this experiment, the use of above mentioned treatments had positive effects on decreasing serum cholesterol and triglyceride, as well as reducing the colonization of Campylobacter jejuni in digestive tract of broiler chickens. | ||
| کلیدواژهها [English] | ||
| challenge, Thyme, yarrow, garlic, organicacids, Antibiotics, Broiler Chickens | ||
| مراجع | ||
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