Effects of Sophora alopecuroides L. extract on AcrAB-TolC pump inhibition in E. coli | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 5, Volume 32, Issue 5 - Serial Number 79, December 2016, Pages 784-794 PDF (206.55 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2016.107995 | ||
| Authors | ||
| P. Mohammadi1; R. Pourahmad* 2 | ||
| 1M.Sc., Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran | ||
| 2Assistant Professor, Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran | ||
| Abstract | ||
| Gram-negative multi-drug resistant bacteria cause untreatable infections. Efflux pumps have a key role in generation of multi-drug resistant bacteria. The inhibition of this pump is a strategy against drug resistance. The aim of this research was to study the effect of Sophorea alopecuroides L. extract on inhibition of AcrAB-TolC pump in E. coli mutants. The extract containing total alkaloids, was used to measure minimum inhibitory concentration (MIC) of extract alone and in combination with ciprofloxacin. Resistant mutants were pretreated with a concentration lower than MIC for different time periods and then MIC was measured for ciprofloxacin. The expression of acrA in mutants treated or untreated with the extract in present and absent of ciprofloxacin was assayed. Results showed that the extract had antibacterial activity by itself and in a concentration lower than MIC in combination with ciprofloxacin had synergistic effect. Meanwhile, a 36-hour pretreatment caused decrease in MIC of ciprofloxacin. Results of real time PCR showed that treatment with extract led to decrease in acrA expression. In conclusion, the extract of Sophorea alopecuroides in E. coli multi drug resistant mutants possibly acts as a pump inhibitor. Therefore, better isolation of plant alkaloids and study their effects could provide a strategy for the synthesis of new drug. | ||
| Keywords | ||
| Herbal drug; Multi-drug resistance; AcrAB-TolC pump; efflux pump inhibitor; Sophora alopecuroides L | ||
| References | ||
|
- Andrews, J.M., 2001. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48(suppl 1): 5-16. - Atta-ur-Rahman, A.U., Choudhary, M.I., Parvez, K., Ahmed, A., Akhtar, F., Nur-E-Alam, M. and Hassan, N.M., 2000. Quinolizidine alkaloids from Sophora alopecuroides. Journal of Natural Products, 63(2): 190-192. - Blair, J.M. and Piddock, L.J., 2009. Structure, function and inhibition of RND efflux pumps in Gram-negative bacteria: an update. Current Opinion in Microbiology, 12(5): 512-519. - Botelho, M., 2000. Fractional inhibitory concentration index of combinations of antibacterial agents against cariogenic organisms. Journal of Dentistry, 28(8): 565-570. - Hächler, H., Cohen, S.P. and Levy, S.B., 1991. marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli. Journal of Bacteriology, 173(17): 5532-5538. - Karczmarczyk, M., Martins, M., Quinn, T., Leonard, N. and Fanning, S., 2011. Mechanisms of fluoroquinolone resistance in Escherichia coli isolates from food-producing animals. Applied and Environmental Microbiology, 77(20): 7113-7120. - Kourtesi, C., Ball, A.R., Huang, Y.Y., Jachak, S.M., Vera, D.M.A., Khondkar, P., Gibbons, S., Hamblin, M. and Tegos, G.P., 2013. Microbial efflux systems and inhibitors: approaches to drug discovery and the challenge of clinical implementation. The Open Microbiology Journal, 7: 34-52. - Küçükboyaci, N., Özkan, S., Adigüzel, N. and Tosun, F., 2011. Characterisation and antimicrobial activity of Sophora alopecuroides L. var. alopecuroides alkaloid extracts. Turkish Journal of Biology, 35(3): 379-385. - Kumar, A. and Schweizer, H.P., 2005. Bacterial resistance to antibiotics: active efflux and reduced uptake. Advanced Drug Delivery Reviews, 57(10): 1486-1513. - Lamers, R.P., Cavallari, J.F. and Burrows, L.L., 2013. The efflux inhibitor phenylalanine-arginine beta-naphthylamide (PAβN) permeabilizes the outer membrane of gram-negative bacteria. PLoS One, 8(3): e60666. - Matsumoto, Y., Hayama, K., Sakakihara, S., Nishino, K., Noji, H., Iino, R. and Yamaguchi, A., 2011. Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels. PLoS One, 6(4): e18547. - Nikaido, H. and Takatsuka, Y., 2009. Mechanisms of RND multidrug efflux pumps. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1794(5): 769-781. - Pfaffl, M.W., Horgan, G.W. and Dempfle, L., 2002. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30(9): e36. - Piddock, L.J., Garvey, M.I., Rahman, M.M. and Gibbons, S., 2010. Natural and synthetic compounds such as trimethoprim behave as inhibitors of efflux in gram-negative bacteria. Journal of Antimicrobial Chemotherapy, 65(6): 1215-1223. - Pourahmad Jaktaji, R. and Ebadi, R., 2013. Generation of clones with higher resistance to tetracycline and chloramphenicol from ciprofluoxacin resistant Escherichia coli mutants. International Research Journal of Applied and Basic Sciences, 4(10): 3063-3067. - Pourahmad Jaktaji, R., Ebadi, R. and Karimi, M., 2012. Study of organic solvent tolerance and increased antibiotic resistance properties in E. coli gyrA mutants. Iranian Journal of Pharmaceutical Research, 11(2): 595-600. - Pourahmad Jaktaji, R. and Jazayeri., N., 2013. Expression of acrA and acrB genes in E. coli mutants with or without marR or acrR mutations. Iranian Journal of Basic Medical Sciences, 16(12): 1254-1258. - Pourahmad Jaktaji, R. and Mohiti, E., 2010. Study of mutations in the DNA gyrase gyrA gene of Escherichia coli. Iranian Journal of Pharmaceutical Research, 9(1): 43-48. - Seeger, M.A., Diederichs, K., Eicher, T., Brandstatter, L., Schiefner, A., Verrey, F. and Pos, K.M., 2008. The AcrB efflux pump: conformational cycling and peristalsis lead to multidrug resistance. Current Drug Targets, 9(9): 729-749. - Soto, S.M., 2013. Role of efflux pumps in the antibiotic resistance of bacteria embedded in a biofilm. Virulence, 4(3): 223-229. - Stavri, M., Piddock, L.J. and Gibbons, S., 2007. Bacterial efflux pump inhibitors from natural sources. Journal of antimicrobial Chemotherapy, 59(6): 1247-1260. - Sun, J., Deng, Z. and Yan, A., 2014. Bacterial multidrug efflux pumps: mechanisms, physiology and pharmacological exploitations. Biochemical and Biophysical Research Communications, 453(2): 254-267. - Szmolka, A. and Nagy, B., 2013. Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Frontiers in Microbiology, 4:1-13. - Tegos, G.P., Haynes, M., Strouse, J.J., Khan, M.M.T., Bologa, C.G., Oprea, T.I. and Sklar, L.A., 2011. Microbial efflux pump inhibition: tactics and strategies. Current Pharmaceutical Design, 17(13): 1291-1302. - Zechini, B. and Versace, I., 2009. Inhibitors of multidrug resistant efflux systems in bacteria. Recent Patents on Anti-infective Drug Discovery, 4(1): 37-50. - Zhou, X., Jia, F., Liu, X. and Wang, Y., 2012. Total Alkaloids of Sophorea alopecuroides‐induced Down‐regulation of AcrAB‐ToLC Efflux pump reverses susceptibility to ciprofloxacin in clinical multidrug resistant Escherichia coli isolates. Phytotherapy Research, 26(11): 1637-1643. - Zhou, X.Z., Jia, F., Liu, X.M., Yang, C., Zhao, L. and Wang, Y.J., 2013. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime. Chinese Journal of Integrative Medicine, 19: 945-949. | ||
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