| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,251 |
| تعداد مقالات | 35,917 |
| تعداد مشاهده مقاله | 54,574,396 |
| تعداد دریافت فایل اصل مقاله | 93,695,642 |
تلفیق نماتدهای بیماری زای حشرات و حشره کش های شیمیایی در کنترل تریپس پیاز Thrips tabaci (Thysanoptera: Thripidae) در شرایط آزمایشگاه (مقاله‌ی انگلیسی) | ||
| آفات و بیماری های گیاهی | ||
| مقاله 15، دوره 84، شماره 2 - شماره پیاپی 103، اسفند 1395، صفحه 1-10 اصل مقاله (368.67 K) | ||
| نوع مقاله: حشره شناسی کشاورزی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2017.107591 | ||
| نویسندگان | ||
| مرضیه کشکولی1؛ نفیسه پورجواد2؛ جهانگیر خواجه علی* 3 | ||
| 1دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه صنعتی اصفهان | ||
| 2استادیار حشره‌شناسی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان | ||
| 3دانشیار حشره‌شناسی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان | ||
| چکیده | ||
| تلفیق نماتدهای بیماری‌زای حشرات و دیگر عوامل کنترلی با ایجاد اثرات افزایشی یا سینرژیستی در مرگ و میر آفت، برای دستیابی به کنترل مطلوب‌تر یک آفت استفاده شده است. در این مطالعه پتانسیل نماتدهای بیماری‌زای حشرات (Steinernema carpocapsae و Heterorhabditis bacteriophora) به تنهایی، حشره‌کش‌های شیمیایی (ایمیداکلوپرید، دلتامترین و آبامکتین) به تنهایی و تلفیق نماتدهاو حشره‌کش‌ها روی تریپس پیاز Thrips tabaci (Thysanoptera: Thripidae) مورد ارزیابی قرار گرفته است. در آزمایش‌های اولیه، اثر غلظت‌های مختلف حشره‌کش‌ها و نماتدها روی لارو تریپس آزمایش و مقادیر LC10 و LC30 حشره‌کش‌ها اندازه‌گیری گردید. سپس با انجام آزمایش‌های پیش‌تیمار، لارو تریپس ابتدا در معرض LC30 آبامکتین (16/5 میلی‌گرم/لیتر)، LC10 ایمیداکلوپرید (16/50 میلی‌گرم/لیتر) یا LC10 دلتامترین (28/10 میلی‌گرم/لیتر) قرار گرفته و سپس با دو غلظت 400 یا 1000 نماتد جوان بیماری‌زا/سانتی‌مترمربع تیمار شد. درصد مرگ و میر در بازه های زمانی مختلف (24، 48 و 72 ساعت پس از استفاده نماتد) ثبت شد. نتایج نشان داد که استفاده از نماتدها به تنهایی کمتر از 30% مرگ و میر تریپس را دنبال داشته است. تلفیق H. bacteriophora و همه حشره‌کش‌ها بطور افزایشی بر مرگ و میر تریپس اثر داشت و تیمار ایمیداکلوپراید اثر سوئی بر عملکرد H. bacteriophora در غلظت 1000 جوان بیماری‌زا/سانتی‌مترمربع نشان داد. اثرات افزایشی و سینرژیستی در تلفیق حشره‌کش‌ها با S. carpocapsae مشاهده شد. این تیمارها در مقایسه با نماتد تنها مرگ و میر بالاتری ایجاد کردند و اثرات سینرژیستی در تلفیق S. carpocapsae غلظت 400 جوان بیماری‌زا/سانتی‌مترمربع با ایمیداکلوپرید و دلتامترین و همچنین غلظت 1000 جوان بیماری-زا/سانتی‌مترمربع با آبامکتین مشاهده گردید. | ||
| کلیدواژه‌ها | ||
| مدیریت تلفیقی آفات؛ Heterorhabditis bacteriophora؛ Steinernema carpocapsae؛ ایمیداکلوپرید؛ آبامکتین | ||
| عنوان مقاله [English] | ||
| Combination of entomopathogenic nematodes and chemical insecticides for controlling the onion thrips, Thrips tabaci (Thysanoptera: Thripidae) in the laboratory condition (Article in English) | ||
| نویسندگان [English] | ||
| M. KASHKOULI1؛ N. POORJAVAD2؛ J. KHAJEHALI3 | ||
| چکیده [English] | ||
| Entomopathogenic nematode (EPN) combinations with other control agents are applied to obtain more desirable control of a pest by additive or synergistic effects on the pest mortality. In this study, the potential of EPNs (Steinernema carpocapsae, and Heterorhabditis bacteriophora) alone, chemical insecticides (imidacloprid, deltamethrin, and abamectin) alone, and EPN-insecticide combinations, against the onion thrips, Thrips tabaci (Thysanoptera: Thripidae) was investigated. In the first experiments, the effects of different concentrations of insecticides and EPNs on the thrips larvae were tested and the LC10 and LC30 values of insecticides were calculated. Then using a pre-treatment bioassay, the thrips larvae were treated with the EPNs at two concentrations, 400 or 1000 infective juveniles (IJs) per cm2, after they had been exposed to LC30 of abamectin (5.16 mg per litre (l), LC10 of imidacloprid (50.16 mg/l), or LC10 of deltamethrin (10.28 mg/l). Mortality percentages were recorded at different time intervals, 24, 48 and 72 hours after the EPNs utilization. Results showed that the sole application of EPNs caused less than 30% thrips mortality. The combination of H. bacteriophora and all insecticides interacted additively on the thrips mortality whereas imidacloprid treatment showed adverse effects on the efficacy of H. bacteriophora at 1000 IJs/cm2. Additive and synergistic interactions resulted in combining insecticides with S. carpocapsae. These combinations gave mostly higher thrips mortality than with EPN alone and synergistic interactions were observed in S. carpocapsae application at 400 IJs/cm2 with imidacloprid and deltamethrin and also at 1000 IJs/cm2 with abamectin. | ||
| کلیدواژه‌ها [English] | ||
| integrated pest management (IPM), Heterorhabditis bacteriophora, Steinernema carpocapsae, Imidacloprid, Abamectin | ||
| مراجع | ||
|
ABBOTT, W. S. 1925. A method of computing the effectiveness of an insecticide, Journal of Economic Entomology, No. 18: 265-267.
ALAUX, C., J. L. BRUNET, C. DUSSAUBAT, F. MONDET, S., TACHAMITCHEN, M. COUSIN, J. BRILLARD, A. BALDY, L. P. BELZUNCES and Y. LE CONTE, 2010. Interactions between Nosema microspores and a neonicotinoid weaken honeybees (Apis mellifera), Environmental Entomology, No.12:774-782.
AL-SIYABI, A. A., M. M. KINAWY, M. AL-ANSRI, A. N. MAHAR, S. R. GOWEN and N. G. HAGUE, 2006. The susceptibility of onion thrips, Thrips tabaci to Heterorhabditis indicus, Communications in Agricultural and Applied Biological Sciences, No. 71: 239-243.
ALUMAI, A. and P. S. GREWAL, 2004. Tank-mix compatibility of the entomopathogenic nematodes, Heterorhabditis bacteriophora and Steinernema carpocapsae, with selected chemical pesticides used in turfgrass, Biocontrol Science and Technology, No. 14: 725-730.
BUITENHUIS SHIPP, J. L. 2005. Efficacy of entomopathogenic nematode Steinernema feltiae (Rhabditida: Steinernematidae) as influenced by Frankliniella occidentalis (Thysanoptera: Thripidae) development stage and host plant stage, Journal of Economic Entomology, No. 98: 1480-1485.
CUTHBERTSON, A. G. S., J. HEAD, K. F. A. WALTERS, and S. A. GREGORY, 2003. The efficacy of the entomopathogenic nematods, Steinernema feltiae, against the immature stages of Bemisia tabaci, Journal of Invertonmental Pathology, No. 83: 267-269.
CUTHBERTSON, A. G. S., J. J. MATHERS, P. NORTHING, A. J. PRICKETT and K. F. A. WALTERS, 2008. The integrated use of chemical insecticides and the entomopathogenic nematodes, Steinernema carpocapsae (Nematoda: Steinernematidae), for the control of sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), Insect Science, No. 15: 447-453.
DE NARDO, E. A. and P. S. GREWAL, 2003. Compatibility of Steinernema feltiae (Nematoda: Steinernematidae) with pesticides and plant growth regulators used in glasshouse plant production, Biocontrol Science and Technology, No. 13: 441-448.
DIAZ-MONTANO, J., M. FUCHS, B. A. NAULT, J. FAIL, and A. M. SHELTON, 2011. Onion thrips (Thysanoptera: Thripidae): a global pest of increasing concern in onion, Journal of Economic Entomology, No. 104: 1-13.
EBSSA, L., C. BORGEMEISTER, O. BERNDT and H. M. POEHLING, 2001. Impact of entomopathogenic nematodes on different soil-dwelling stages of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), in the laboratory and undes semi-field conditions, Biocontrol Science and Technology, No. 11: 515-525.
EBSSA, L., C. BORGEMEISTER and H. M. POEHLING, 2003. Effectiveness of different species/strains of entomopathogenic nematodes for control of western flower thrips (Frankliniella occidentalis) at various concentrations, host densities and temperatures, Biological Control, No. 29: 145-154.
EBSSA, L., C. BORGEMEISTER and H. M. POEHLING, 2006. Simultaneous application of entomopathogenic nematodes and predatory mites to control western flower thrips Frankliniella occidentalis, Biological Control, No. 39: 66-74.
EHLERS, R. U. and D. L. SHAPIRO-ILAN, 2005. Mass production. 65-79 In: Nematodes as biocontrol agents. (Eds). Grewal, P. S., Ehlers, R. U. and Shapiro-Ilan, D. I. CAB International, Wallingford.
ELAD, Y., I. PERTOT and A. ENKEGAARD, 2004. Combined use of insect pathogenic fungi and nematodes against the onion thrips, Thrips tabaci, in the field, Bulletin OILB-SROP, No. 27: 141-143.
ELIMEM, M., D. A. TEIXEIRA, J. A. SILVA, and B. CHERMITI, 2014. Double-attraction method to control Frankliniella occidentalis (Pergande) in pepper crops in Tunisia, Plant Protectection Science, No. 50: 90–96.
GARCIA-DEL-PINO, F., X. ALABERN and A. MORTON, 2013. Efficacy of soil treatments of entomopathogenic nematodes against the larvae, pupae and adults of Tuta absoluta and their interaction with the insecticides used against this insect, BioControl, No, 58: 723-731.
HEAD, J., K. F. A. WALTERS and S. LANGTON, 2000. The compatibility of the entomopathogenic nematode, Steinernema feltiae, and chemical insecticides for the control of the South American leafminer, Liriomyza huidobrensis, BioControl, No. 45: 345-353.
JAMES, R. R. and J. XU, 2012. Mechanisms by which pesticides affect insect immunity, Journal of Invertebrate Pathology, No. 109: 175-182.
JENSEN, S. E. 2000. Insecticide resistance in the western flower thrips, Frankliniella occidentalis, Integrated Pest Management Reviews, No. 5: 131-146.
JONES, D. R. 2005. Plant viruses transmitted by thrips, European Journal of Plant Pathology, No. 113: 119-157.
KASHKOULI, M., J. KHAJEHALI and N. POORJAVAD, 2014. Impact of entomopathogenic nematodes on Thrips tabaci Lindeman (Thysanoptera: Thripidae) life stages in the laboratory and under semi –field conditions, Journal of Biopesticides, No. 7: 77-84.
KAYA, H. K. and R. GAUGLER, 1993. Entomopathogenic nematodes, Annual Review of Entomology, No. 38: 181-206.
KAYA, H. K. and S. P. STOCK, 1997. Techniques in insect nematology. 281-324 In: Manual of Techniques in Insect Pathology. (ED). Lacy, L. A., San Diego, Academic Press.
KOPPENHOFER, A. M., I. M. BROWN, R. GAUGLER, P. S. GREWAL, H. K. KAYA and M. G. KLEIN, 2000a. Synergism of entomopathogenic nematodes and imidacloprid against white grubs: greenhouse and field evaluation, Biological Control, No. 19: 245-252.
KOPPENHOFER, A. M., R. S. COWLES, E. A. COWLES, E. M. FUZY and L. BAUMGARTNER, 2002. Comparison of neonicotinoid insecticides as synergists for entomopathogenic nematodes, Biological Control, No. 24: 90-97.
KOPPENHOFER, A. M., P. S. GREWAL and H. K. KAYA, 2000b. Synergism of imidacloprid and entomopathogenic nematodes against white grubs: the mechanism, Entomologia Experimentalis et Applicata, No. 94: 283-293.
KOPPENHOFER, A. M. and P. S. GREWAL, 2005. Compatibility and interactions with agrochemicals and other biocontrol agents. 147-166 In: Nematodes as biocontrol agents. (Eds). Grewal, P. S., Ehlers, R. U. and Shapiro-Ilan, D. I. CAB International, Wallingford.
KRISHNAYYAAND, P. V. and P. S. GREWAL, 2002. Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematode Steinernema feltiae, Biocontrol Science and Technology, No. 12: 259-266.
LALL, B. and L. SINCH, 1986. Biology and control of the onion thrips in India, Journal of Economic Entomology, No. 61: 676-679.
LOOMANS, A. J. M. and T. MURAI, 1997. Culturing thrips and parasitoids. 477-503 In: Thrips as crop pests. (Ed). Lewis, T. CAB International, Harpenden, Herts, UK.
MORTIZ, G. 1997. Structure, growth and development. 15-64 In: Thrips as crop pests. (Ed). Lewis, T. CAB International, Harpenden, Herts, UK.
OPARAEKE, A. M. 2006. The sensitivity of flower bud thrips, Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae), on cowpea to three concentrations and spraying schedules of Piper guineense Schum. & Thonn. Extracts, Plant Protection Science, No. 42: 106–111.
PREMACHANDRA, W. T. S. D., C. BORGEMEISTER, O. BERNDT, R. U. EHLERS and H. M. POEHLING, 2003. Combined release of entomopathogenic nematodes and the predatory mite Hypoaspis aculeifer to control soil dwelling stages of western flower thrips Frankliniella occidentalis, BioControl, No. 48: 529-541.
SAFFARI, T., H. MADADI and J. KARIMI, 2013. Pathogenicity of three entomopathogenic nematodes against the onion thrips, Thrips tabaci Lind. (Thys.; Thripidae), Archives of Phytopathology and Plant Protection, No. 46(20): 2459-2468.
SAS INSTITUTE, 2008. SAS/STAT®, release 9.2 Users Guide. Cary, NC.
SMITH, R. M., A. G. CUTHBERTSON and K. F. WALTERS, 2005. Note: Extrapolating the use of an entomopathogenic nematode and fungus as control agents for Frankliniella occidentalis to Thrips palmi, Phytoparasitica, No. 3: 436-440.
TOMALAK, M., S. PIGGOT and G. B. JAGDALE, 2005. Glasshouse applications. 147-166 In: Nematodes as biocontrol agents. (Eds). P. S. Grewal, P. S., Ehlers, R. U. and Shapiro-Ilan D. I. CAB International, Wallingford.
WILLIAMS, E. C. and K. F. A. WALTERS, 2000. Foliar application of the entomopathogenic nematode Steinernema feltiae against leafminers on vegetables, Biocontrol Science and Technology, No. 10 : 61-70.
WORKMAN, P. J. and N. A. MARTIN, 2002. Towards integrated pest management of Thrips tabaci in onions, New Zealand Plant Protection, No. 55: 188-192.
YU, S. J. 2008. The Toxicology and Biochemistry of insecticides, New York, NY: CRC. | ||
|
آمار تعداد مشاهده مقاله: 1,619 تعداد دریافت فایل اصل مقاله: 1,126 |
||