| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,533 |
| تعداد مشاهده مقاله | 49,381,139 |
| تعداد دریافت فایل اصل مقاله | 79,831,871 |
Effects of estradiol and oxytocin injection on the efficiency of artificial insemination in Iranian Zel ewes during the breeding season | ||
| Archives of Razi Institute | ||
| مقاله 5، دوره 72، شماره 1، خرداد 2017، صفحه 33-41 اصل مقاله (156.49 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.107489 | ||
| نویسندگان | ||
| Afshin Seifi-Jamadi1؛ Reza Masoudi1؛ A.R. Hosseinzadeh Aski2؛ Hamid Kohram* 3؛ Mohsen Sharafi4؛ Seyed Danial Moein Aledavoud5؛ Hassan sadeghipanah2 | ||
| 1Department of Animal Sciences, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Ghaemshahr Branch, Ghaemshahr, Iran | ||
| 3Department of Animal Sciences, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran; Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran | ||
| 4Department of Animal Sciences, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 5Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| چکیده | ||
| In sheep industry, pregnancy rate after artificial insemination (AI) declines due to the complex anatomy of the cervix in ewes, such that it might prevent effective intrauterine insemination. At estrus, cervical relaxation occurs to some degree in ewes, which is regulated by the changes in the levels of reproductive hormones. This study aimed to evaluate the effects of estradiol and intravenous (IV) or intramuscular (IM) oxytocin injection at different doses on the cervical opening and pregnancy rate of Iranian Zel ewes during the breeding season. For this purpose, three experiments were conducted on 120 ewes (3-4 years old, weighing 47±2.5 kg). In the first experiment, ewes were equally assigned to two groups to receive estradiol (100-200 µg). After 12 h, each group was equally divided into six subgroups (n=20) and received 50, 100 and 150 IU oxytocin via IV and IM injection. Cervical opening was measured before and 15 min and 12 h after estradiol injection and 20 min after oxytocin administration. In the second experiment, we only assessed the effect of oxytocin administration on cervical opening similar to the first experiment. In the third experiment, controlled internal drug release (CIDR) was used in all the ewes for 12 days to induce estrus synchronization. Afterwards, the ewes received 550 IU intrauterine equine chorionic gonadotropin at the time of CIDR removal. Before AI, ewes were equally categorized into three groups (n=40); the first group was considered as control, and the other two groups received 100 IU oxytocin via IM or IV injection. At 54 h after CIDR removal, all ewes were inseminated transcervically using diluted fresh semen. Pregnancy was detected via ultrasound 50 days after insemination, and lambing and twinning rates were measured after parturition. Results of the first and second experiment indicated that estradiol injection had no effect on cervical opening (P>0.05), while the administration of 100 or 150 IU oxytocin (IV or IM) could dilate the cervix with or without estradiol (P<0.05). Furthermore, administration of 100 IU oxytocin (IV or IM) in the third experiment improved pregnancy and lambing rates compared to the control group (P<0.05); however, it had no effect on the twinning rate of the ewes (P>0.05). According to the results, IV or IM injection of oxytocin could improve the pregnancy rate in Iranian Zel ewes through the dilation of cervical canal. Therefore, it is suggested that this method be applied to enhance the pregnancy rate of ewes during the breeding season. | ||
| کلیدواژهها | ||
| Cervical dilation؛ Estradiol؛ Oxytocin؛ Pregnancy rate؛ artificial insemination | ||
| عنوان مقاله [English] | ||
| بهبود بازده تولید مثلی در گوسفندان ایرانی نژاد زل با استفاده از تزریق هورمونهای استرادیول و اکسی توسین در فصل تولید مثلی | ||
| نویسندگان [English] | ||
| افشین سیفی جمادی1؛ رضا مسعودی1؛ علیرضا حسینزاده اسکی2؛ حمید کهرام3؛ محسن شرفی4؛ سید دانیال معین آل داوود5؛ حسن صادقی پناه2 | ||
| 1گروه علوم جانوری، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، ایران | ||
| 2گروه علوم دامی دانشکده کشاورزی دانشگاه آزاد اسلامی، شعبه قائم شهر | ||
| 3گروه علوم جانوری، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، ایران، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4گروه علوم جانوری، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، ایران گروه علوم طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 5گروه علوم طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده [English] | ||
| در این مطالعه از میشهای سه تا چهار ساله با میانگین وزنی 5/2± 47 کیلوگرم استفاده شد. در آزمایش اول میشها به دو گروه مساوی (n=120) تقسیم شده و به ترتیب به آنها مقدار 100 و 200 میکرولیتر استرادیول تزریق شد. 12 ساعت پس از تزریق استرادیول میش های هر گروه به شش زیر گروه مساوی (n=20) تقسیم شدند و مقدار 50 ، 100 و 150 واحد اکسیتوسین را به صورت درون وریدی یا درون عضلانی دریافت نمودند. میزان نفوذ به سرویکس قبل از تزریق، 15 دقیقه و 12 ساعت پس از تزریق استرادیول (قبل از تزریق اکسیتوسین) و همچنین 20 دقیقه پس از تزریق اکسیتوسین با پیپت مدرج تلقیح مصنوعی اندازهگیری شد. آزمایش دوم مانند آزمایش اول بود با این تفاوت که به میشها استرادیول تزریق نشد و فقط اثر اکسیتوسین بر باز شدن سرویکس تعیین شد. در آزمایش سوم میشها (n=120) برای همزمانی فحلی به مدت 12 روز سیدرگذاری شدند و در هنگام سیدربرداری به همه میشها مقدار 550 واحد هورمون eCG تزریق شد. قبل از تلقیح مصنوعی میشها به سه گروه مساوی تقسیم شدند. گروه اول به عنوان گروه شاهد انتخاب شد و هورمون اکسیتوسین دریافت نکرد. دو گروه دیگر به دو دسته تزریق درونعضلانی و تزریق درونوریدی تقسیم شدند و 100 واحد هورمون اکسیتوسین را دریافت کردند. تمام میشها 54 ساعت. استرادیول به تنهایی اثری بر باز شدن سرویکس نداشت (05/0P<) ولی تزریق عضلانی و یا وریدی اکسیتوسین با دوزهای 100 و 150 واحد در حضور و یا عدم حضور استرادیول باعث بازشدن سرویکس در میشهای زل شد(05/0P>). | ||
| کلیدواژهها [English] | ||
| اتساع سرویکس, استرادیول, اکسیتوسین, درصد آبستنی | ||
| مراجع | ||
|
Akinbami, M.A., Meredith, S., Warren, J.E., Jr., Anthony, R.V., Day, B.N., 1990. Cervical dilation, conception rate, and concentrations of progesterone and estradiol-17B in postpartum ewes treated with porcine relaxin. Theriogenology 34, 927-940. Anel, L., Alvarez, M., Martinez-Pastor, F., Garcia-Macias, V., Anel, E., de Paz, P., 2006. Improvement strategies in ovine artificial insemination. Reprod Domest Anim 41 Suppl 2, 30-42. Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E., Boixo, J.C., et al., 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology 63, 1235-1247. Ayad, V.J., Leung, S.T., Parkinson, T.J., Wathes, D.C., 2004. Coincident increases in oxytocin receptor expression and EMG responsiveness to oxytocin in the ovine cervix at oestrus. Anim Reprod Sci 80, 237-250. Buckrell, B.C., Buschbeck, C., Gartley, C.J., Kroetsch, T., McCutcheon, W., Martin, J., et al., 1994. Further development of a transcervical technique for artificial insemination in sheep using previously frozen semen. Theriogenology 42, 601-611. Cabrol, D., Dallot, E., Cedard, L., Sureau, C., 1987. Pregnancy-related changes in the distribution of glycosaminoglycans in the cervix and corpus of the human uterus. European Journal of Obstetrics and Gynecology and Reproductive Biology 20, 289-295. Croy, B.A., Prudencio, J., Minhas, K., Ashkar, A.A., Galligan, C., Foster, R.A., et al., 1999. A preliminary study on the usefulness of huil-8 in cervical relaxation of the ewe for artificial insemination and for embryo transfer. Theriogenology 52, 271-287. El Maradny, E., Kanayama, N., Kobayashi, H., Hossain, B., Khatun, S., Liping, S., et al., 1997. The role of hyaluronic acid as a mediator and regulator of cervical ripening. Human Reproduction 12, 1080-1088. Eppleston, J., Salamon, S., Moore, N.W., Evans, G., 1994. The depth of cervical insemination and site of intrauterine insemination and their relationship to the fertility of frozen-thawed ram semen. Animal Reproduction Science 36, 211-225. Flohr, S.F., Wulster-Radcliffe, M.C., Lewis, G.S., 1999. Technical note: development of a transcervical oocyte recovery procedure for sheep. J Anim Sci 77, 2583-2586. Halbert, G.W., Dobson, H., Walton, J.S., Buckrell, B.C., 1990. A technique for transcervical intrauterine insemination of ewes. Theriogenology 33, 993-1010. Hawk, H.W., 1983. Sperm Survival and Transport in the Female Reproductive Tract. Journal of Dairy Science 66, 2645-2660. Kershaw-Young, C.M., Khalid, M., McGowan, M.R., Pitsillides, A.A., Scaramuzzi, R.J., 2009. The mRNA expression of prostaglandin E receptors EP2 and EP4 and the changes in glycosaminoglycans in the sheep cervix during the estrous cycle. Theriogenology 72, 251-261. Kumaresan, A., Chand, S., Suresh, S., Mohanty, T.K., Prasad, S., Layek, S.S., et al., 2013. Effect of estradiol and cloprostenol combination therapy on expulsion of mummified fetus and subsequent fertility in four crossbred cows. Vet Res Forum 4, 85-89. Ledger, W.L., Ellwood, D.A., Taylor, M.J., 1983. Cervical softening in late pregnant sheep by infusion of prostaglandin E-2 into a cervical artery. J Reprod Fertil 69, 511-515. Leethongdee, S., Khalid, M., Bhatti, A., Ponglowhapan, S., Kershaw, C.M., Scaramuzzi, R.J., 2007. The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period. Theriogenology 67, 767-777. Lightfoot, R.J., Salamon, S., 1970. Fertility of ram spermatozoa frozen by the pellet method. I. Transport and viability of spermatozoa within the genital tract of the ewe. J Reprod Fertil 22, 385-398. Masoudi, R., Kohram, H., Shahne, A.Z., Davoud, S.D.M.A., 2012a. Effect of estradiol and oxytocin on ovine cervical relaxation. African J Biotechnol 11, 2803-2806 Masoudi, R., Kohram, H., Shahne, A.Z., Davoud, S.D.M.A., Sharif, A.A., 2012b. Effects of exogenous oxytocin on cervical penetration of Iranian ewes. African J Biotechnol 11, 2398-2401. Mitchell, S.E., Robinson, J.J., King, M.E., McKelvey, W.A., Williams, L.M., 2002. Interleukin 8 in the cervix of non-pregnant ewes. Reproduction 124, 409-416. Mylne, M.J., McKelvey, W.A., Fernie, K., Matthews, K., 1992. Use of a transcervical technique for embryo recovery in sheep. Vet Rec 130, 450-451. Perry, K., Haresign, W., Wathes, D.C., Khalid, M., 2010. Intracervical application of hyaluronan improves cervical relaxation in the ewe. Theriogenology 74, 1685-1690. Sayre, B.L., Lewis, G.S., 1997. Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes. Theriogenology 48, 267-275. Shemesh, M., Dombrovski, L., Gurevich, M., Shore, L.S., Fuchs, A.-R., Fields, M.J., 1997. Regulation of bovine cervical secretion of prostaglandins and synthesis of cyclooxygenase by oxytocin. Reproduction, Fertility and Development 9, 525-530. Stellflug, J.N., Wulster-Radcliffe, M.C., Hensley, E.L., Cowardin, E.A., Seals, R.C., Lewis, G.S., 2001. Oxytocin-induced cervical dilation and cervical manipulation in sheep: effects on laparoscopic artificial insemination. J Anim Sci 79, 568-573. Stys, S.J., Dresser, B.L., Otte, T.E., Clark, K.E., 1981. Effect of prostaglandin on cervical compliance in pregnant ewes. American Journal of Obstetrics & Gynecology 140, 415-419. Torres, S., Sevellec, C., 1987. Repeated superovulation and surgical recovery of embryos in the ewe. Reprod. Nutr. Dévelop. 27, 859-863. Viudes-de-Castro, M.P., Salvador, I., Marco-Jimenez, F., Gomez, E.A., Silvestre, M.A., 2009. Effect of oxytocin treatment on artificial insemination with frozen-thawed semen in Murciano-Granadina goats. Reprod Domest Anim 44, 576-579. Wulster-Radcliffe, M.C., Lewis, G.S., 2002. Development of a new transcervical artificial insemination method for sheep: effects of a new transcervical artificial insemination catheter and traversing the cervix on semen quality and fertility. Theriogenology 58, 1361-1371. Wulster-Radcliffe, M.C., Wang, S., Lewis, G.S., 2004. Transcervical artificial insemination in sheep: effects of a new transcervical artificial insemination instrument and traversing the cervix on pregnancy and lambing rates. Theriogenology 62, 990-1002. | ||
|
آمار تعداد مشاهده مقاله: 2,073 تعداد دریافت فایل اصل مقاله: 2,986 |
||