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Characterization of digestive α- and β-glucosidases in the midgut of Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae) | ||
| Journal of Entomological Society of Iran | ||
| مقاله 6، دوره 35، شماره 4، خرداد 2016، صفحه 45-51 اصل مقاله (567.19 K) | ||
| نویسندگان | ||
| M. Shabarari؛ B. Naseri* | ||
| Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| چکیده | ||
| The imported willow leaf beetle, Plagiodera versicolora is one of the main pests of forest trees worldwide including Iran. Activities of α- and β-glucosidases in the midgut of P. versicolora adults were studied in this research. The results showed a higher α- glucosidase activity of adultsthan β-glucosidase. The optimal pHs for α- and β-glucosidases activities were found to be 6 and 9, respectively. Optimal temperatures for the enzymatic activities were recorded at 30 and 25 °C, respectively. Concentration of 0.5 mM K+ significantly minimized the activity of α- glucosidase and maximized the activity of α-glucosidase in presence of Ca2+. The activity of β-glucosidase was decreased by adding Mg2+ and Zn2+. 5 mM concentration of K+ had the most inhibitory impact on β-glucosidase activity, while 0.5 mM concentration of Na+ showed the most positive effect on the enzyme activity. The Km of midgut α- and β- glucosidase was 0.36 and 2.03 mM and the Vmax value was 5.46 and 18.51 μmol/min/mg protein, respectively. | ||
| کلیدواژهها | ||
| Plagiodera versicolora؛ α-glucosidase؛ β-glucosidase؛ Iran؛ Midgut | ||
| عنوان مقاله [English] | ||
| Characterization of digestive α- and β-glucosidases in the midgut of Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae) | ||
| نویسندگان [English] | ||
| میسره شابراری؛ بهرام ناصری | ||
| گروه گیاهپزشکی، دانشکدهی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| چکیده [English] | ||
| سوسک برگخوار بید Plagiodera versicolora یکی از آفات مهم درختان جنگلی در دنیا از جمله ایران میباشد. در پژوهش حاضر، فعالیت آلفا و بتا- گلوکوزیداز در معده میانی حشره کاملP. versicolora بررسی شد. نتایج نشان داد که فعالیت آلفا- گلوکوزیداز بیشتر از بتا-گلوکوزیداز بود. pH بهینه برای فعالیت آلفا و بتا- گلوکوزیداز بهترتیب 6 و 9 بهدست آمد. همچنین دمای بهینه فعالیت این دو آنزیم بهترتیب 30 و 25 درجه سلسیوس محاسبه شد. غلظت 5/0 میلیمولار یون K+بیشترین تأثیر را در کاهش فعالیت آنزیم آلفا- گلوکوزیداز داشت و بهینه فعالیت آلفا- گلوکوزیداز در حضور یون Ca2+مشاهده شد. فعالیت آنزیم بتا- گلوکوزیداز با اضافه کردن یونهای Mg2+وZn2+کاهش یافت. علاوه بر این، غلظت 5 میلیمولار یون K+ بیشترین تأثیر بازدارندگی را بر فعالیت بتا- گلوکوزیداز داشت و یون +Na (غلظت 5/0 میلیمولار) بیشترین تأثیر مثبت را بر فعالیت آنزیم داشت. مقدار Km آلفا و بتا گلوکوزیداز بهترتیب 36/0 و 03/2 میلیمولار و مقدار Vmax بهترتیب46/5 و 51/18 میکرومول بر دقیقه بر میلیگرم پروتئین بهدست آمد. | ||
| کلیدواژهها [English] | ||
| Plagiodera versicolora, آلفا- گلوکوزیداز, بتا- گلوکوزیداز, ایران, معده میانی | ||
| مراجع | ||
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