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اثر ضدباکتریایی چند اسانس گیاه دارویی درکنترل باکتری‌های عامل پوسیدگی و پژمردگی سیب‌زمینی در شرایط آزمایشگاه و گلخانه | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 9، دوره 32، شماره 4 - شماره پیاپی 78، آبان 1395، صفحه 645-654 اصل مقاله (192.14 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.107136 | ||
| نویسندگان | ||
| لیلی علمشاهی* 1؛ مرضیه حسینی‌نژاد2 | ||
| 1دانش‌آموخته کارشناسی ارشد بیماری‌شناسی گیاهی، دانشگاه زابل، ایران | ||
| 2استادیار، پژوهشکده علوم و صنایع غذایی، مشهد، ایران | ||
| چکیده | ||
| امروزه استفاده از اسانس‌های گیاهی برای دستیابی به مواد ضدباکتریایی طبیعی برای کنترل عوامل بیماری‌زای گیاهی مورد توجه فراوان قرار گرفته است. در این تحقیق اثر بازدارندگی اسانس پنج گیاه آویشن باغی (Thymus sativum L.)، گشنیز (Coriandrum sativum L.)، زیره سبز (Cuminum cyminum L.)، رزماری (Rosmarinus officinalis L.) و اکالیپتوس(Eucalyptus globules L.) با روش تقطیر با آب استخراج و اثر بازدارندگی آنها بر روی باکتری‌های Ralstonia solanacearu (عامل پوسیدگی نرم سیب‌زمینی) و Pectobacterium carotovorum (عامل پژمردگی سیب‌زمینی) با روش دیسک دیفیوژن آزمایش شد. برای تعیین حداقل غلظت بازدارندگی و حداقل غلظت باکتری‌کشی از روش رقت لوله‌ای استفاده شد. آزمایش‌ها در قالب طرح فاکتوریل کاملاً تصادفی تجزیه واریانس گردید. مقایسه میانگین‌ها براساس آزمون چند دامنه‌ای دانکن (نرم‌افزار MSTAT-C) انجام شد. در مرحله گلخانه‌ای از اسانس آویشن باغی که بیشترین مقدار بازدارندگی را بر روی هر دو باکتری در مرحله آزمایشگاهی نشان داده بود، استفاده گردید. نتایج نشان داد که اسانس آویشن باغی با ایجاد هاله بازدارندگی به قطر 8/34 میلی‌متر در مقایسه با سایر اسانس‌ها دارای بیشترین اثر و فعالیت ضدباکتریایی بود. برای آزمایش‌های گلخانه‌ای از اسانس آویشن با غلظت‌های 5/0% به روش اسپری و 1% به روش ریختن اسانس به خاک استفاده شد. نتایج نشان داد که آویشن باغی به میزان 41% وقوع بیماری پوسیدگی نرم و 44% وقوع پژمردگی باکتریایی را در سیب‌زمینی کاهش داد. بنابراین اسانس آویشن باغی می‌تواند با داشتن قابلیت ضدباکتریایی مناسب، به‌عنوان عامل بازدارنده در مدیریت این دو بیماری در گیاه سیب‌زمینی بکار گرفته شود. | ||
| کلیدواژه‌ها | ||
| اثر ضدباکتریایی؛ اسانس گیاهی؛ Ralstonia solanacearum؛ Pectobacterium carotovorum | ||
| عنوان مقاله [English] | ||
| Inhibitory effects of essential oils of some medicinal plants on the causal agents bacteria of potato wilt and soft rot under laboratory and greenhouse conditions | ||
| نویسندگان [English] | ||
| L. Alamshahi1؛ M. Hosseini Nezhad2 | ||
| 1M.Sc. in Plant Diseases, College of Agriculture, Zabol University, Zabol, Iran | ||
| 2Research Institute of Food Science and Technology (RIFST), Mashhad, Iran | ||
| چکیده [English] | ||
| Nowadays, using essential oils as natural antibacterial inhibitors to control of plant diseases has been widely investigated. In the current research, the essential oils of Coriandrum sativum L., Thymus sativum L., Cuminum cyminum L., Rosmarinus officinalis L. and Eucalyptus globulus L. were extracted by water distillation method and then the inhibitory effects were tested against Pectobacterium carotovorum (causal agent of bacterial wilt in potato) andRalstonia solanacearum (causal agent of softrot in potato) using paper disk diffusion method. The minimum inhibitory concentration and minimum bactericidal concentration were determined by twofold broth dilution method. The experiments were analyzed by a completely randomized factorial design. Duncan’s multiple range test (MSTATC software) was used for comparison of means. Thyme oil exhibited the highest antibacterial activity in cultured media for both plant pathogenic bacteria, so it was selected to be applied in greenhouse experiments. The results showed that T. vulgaris had the most inhibition zone compared to the other oils in cultured media with 34.8 mm. In the greenhouse experiments, thyme oil was used for plant spraying and soil drenching methods at concentration of 0.5 and %1, respectively. According to the results, the thyme essential oil caused a significant reduction in soft rot and bacterial wilt incidence on potato by 41 and 44%, respectively compared with the control sample. Generally, thyme oil with a suitable antibacterial potential would be considered as an inhibitory agent to management of two studied diseases on potatoes. | ||
| کلیدواژه‌ها [English] | ||
| Antibacterial effects, Essential oils, Ralstonia solanacearum, Pectobacterium carotovorum | ||
| مراجع | ||
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