Identification of the fungal pathogens causing leaf spot on barnyardgrass (Echinochloa crus-galli) in rice paddy fields of Mazandaran | ||
| آفات و بیماری های گیاهی | ||
| Article 5, Volume 84, No. 2 - Serial Number 103, March 2017, Pages 261-268 PDF (1.73 M) | ||
| Document Type: Plant Pathology | ||
| DOI: 10.22092/jaep.2017.107096 | ||
| Authors | ||
| F. BAGHERI; S. NAEIMI* | ||
| Abstract | ||
| Barnyardgrass is the most important weed in paddy fields of northern Iran. In order to identify the fungal pathogens causing leaf spot on this dominant weed, symptomatic plants were collected from rice fields in different areas of Mazandaran province. Leaf pieces of ca 0.5×0.5 cm from the margins of diseased spots were surface sterilized in 0.5% sodium hypochlorite and washed with sterile distilled water. The pieces were placed on water agar 2% or PDA supplemented with streptomycin sulfate. All Petri dishes were incubated at 25°C in darkness. The grown fungal colonies were isolated and purified using single spore or hyphal tip methods. To determine their pathogenicity, detached-leaf assay and spraying spore suspension on whole plants in greenhouse were used. The fungal isolates which caused symptoms, considered for further studies. Morphological identification of pathogenic isolates was performed using standard keys and monographs. In addition, genomic DNA was extracted and a nuclear rDNA region was amplified. The resulting sequences were submitted to a BLAST search to find most similar sequences in NCBI GenBank. Overall, based on morphological and molecular data, the identified fungal pathogens were Alternaria alternata, Bipolaris oryzae, Bipolaris sorokiniana, Curvularia lunata and Rhizoctonia solani. | ||
| Keywords | ||
| rice; barnyardgrass; disease; Iran | ||
| References | ||
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