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Prevalence and phylogenetic analysis of Theileria equi in Iranian dromedaries | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 71، شماره 3، آذر 2016، صفحه 169-175 اصل مقاله (273.89 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.106970 | ||
| نویسندگان | ||
| S. Bahrami* 1؛ M.R. Tabandeh2؛ A. Nikbin1؛ A.R. Alborzi1؛ A.R. Ghadrdan3 | ||
| 1Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Considering the importance of Theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of T. equi among dromedaries in central Iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. For this purpose, a total of 161 blood samples from camels were examined in terms of T. equi infection, using parasitological and molecular methods. For molecular detection of T. equi, primers targeting the 18S rRNA gene were selected. Microscopic examination revealed that 0.6% of camels were positive for the intraerythrocytic stage of Theileria species, while polymerase chain reaction (PCR) method detected T. equi in 7 (4.3%) out of 161 camels. Sequences of 18S rRNAs from all the isolates showed more than 99% homology to each other and T. equi isolates in the GenBank. With respect to the single-nucleotide substitution in 18S rRNA gene of the studied camels, three different genotypes were identified and submitted to the GenBank. Considering the homology between 18S rRNA sequences of T. equi in the studied samples and those available in the GenBank, the phylogenetic tree formed three distinct, but highly-related clusters. In this study, age, gender, and locality were not determined as risk factors for T. equi infection in camels. In conclusion, this study demonstrated that T. equi is present among Iranian camels. | ||
| کلیدواژهها | ||
| Theileria equi؛ Camel؛ Polymerase Chain Reaction؛ Phylogenetic analysis؛ Yazd | ||
| عنوان مقاله [English] | ||
| شناسایی مولکولی و آنالیز فیلوژنیک تیلریا اکوئی در شترهای یزد (Camelus dromedarius) | ||
| نویسندگان [English] | ||
| سمیه بهرامی1؛ محمدرضا تابنده2؛ علی نیکبین1؛ علیرضا البرزی1؛ علیرضا قدردان3 | ||
| 1گروه انگل شناسی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| 2گروه بیو شیمی و مولکولار بیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| 3گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| چکیده [English] | ||
| با توجه به اهمیت عفونت تیلریا اکویی در صنعت تولید و صادرات اسب، هدف از مطالعه حاضر بررسی حضور تیلریا اکویی در شترهای مرکز ایران، مکانی که در آن جا جمعیت قابل توجهی از شتر و اسب وجود دارد و نیز تیلریوز اسبی شایع است بوده است. در مجموع نمونه خون 161 شتر به روش انگلشناسی و مولکولی برای بررسی حضور انگل تیلریا اکوئی بررسی گردید. برای جستجوی تیلریا اکوئی، پرایمرهایی که هدف آنها ژن 18s rRNA بود انتخاب گردید. مطالعهی میکروسکوپیک نشان داد که 6/0 درصد شترها برای مرحله درون گلبول قرمزی انگل تیلریا مثبت بودهاند در حالی که از 161 نمونه، 7 نمونه (3/4%) با استفاده از روش واکنش زنجیرهای پلیمراز، آلوده به تیلریا اکویی تشخیص داده شد. توالی ژن 18s rRNA تمام جدایهها، بیش از 99 درصد شباهت با یکدیگر و تیلریا اکوییجدا شده از اسبها و شترهای مطالعات دیگر داشته است. بر اساس جایگزینی تک نوکلئوتیدی در ژن 18s rRNA از شترهای مورد مطالعه، 3 ژنوتیپ مختلف شناسایی و به بانک ژن ارائه شد. بر اساس همولوژیهای بین توالی 18s rRNA تیلریا اکویی به دست آمده از شترها و اسبهای مطالعه و ارائه شده به بانک ژن، درخت فیلوژنی 3 شاخه بسیار مرتبط مجزا تشکیل داد. سن، جنس و محل جغرافیایی به عنوان عوامل خطر آلودگی به تیلریا اکویی در شترهای این مطالعه مشخص نشد. در پایان، این مطالعه نشان داد که در حال حاضر شترهای ایران به تیلریا اکویی آلوده هستند | ||
| کلیدواژهها [English] | ||
| تیلریا اکوئی, واکنش زنجیرهای پلیمراز, آنالیز فیلوژنیک, یزد | ||
| مراجع | ||
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