News

a.z
Manaffar, R., Bageri, B., Rahimi, S., Diba, K. (2016). Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses. , 29(4), 119-127. doi: 10.22034/vj.2016.106788
R. Manaffar; B. Bageri; S. Rahimi; K. Diba. "Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses". , 29, 4, 2016, 119-127. doi: 10.22034/vj.2016.106788
Manaffar, R., Bageri, B., Rahimi, S., Diba, K. (2016). 'Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses', , 29(4), pp. 119-127. doi: 10.22034/vj.2016.106788
Manaffar, R., Bageri, B., Rahimi, S., Diba, K. Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses. , 2016; 29(4): 119-127. doi: 10.22034/vj.2016.106788

Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 15, Volume 29, Issue 4, December 2016, Pages 119-127    PDF (2.33 M)
Document Type: Full Research Paper
DOI: 10.22034/vj.2016.106788
Authors
R. Manaffar* 1; B. Bageri2; S. Rahimi2; K. Diba3
1Assistant professor of Department of Fisheries, Faculty of Natural Resources, Urmia University, Iran
2Urmia Lake Research Institute, Urmia University, Iran.
3Assistant professor of Department of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Iran.
Abstract
Unicellular yeasts are known as a part of aquatics intestinal flora. Some species are useful and some are known as pathogen for known or unknown diseases. So far any comprehensive study was done to characterize yeast intestine flora of fish in Iran. Due to the importance of isolation and characterization of micro flora of fishes and possibility of introducing this endemic species as Probiotics, in present study, the diversity of the intestinal yeast flora of domestic Rainbow trout fishes of 6 Urmia cities farms was investigated by general and specific diffraction media as SDA, SCC, CHA and CMA following to molecular analyses. Molecular technique of PCR for small fragment of ITS1 was followed by sequencing in order to identifying some complex samples in case of culture methods failure. This study not only succeed to stablishe a protocol for characterization of yeast micro flora of fishes but also could identify some species Candida guilliermondii, Saccharomyces cerevisiae strain LN, Saccharomyces cerevisiae strain YG3-1, Saccharomyces cerevisiae and also strains of Rhodotorula.
 
Keywords
Intestine flora; Isolation; Rainbow trout; Media; Yeast
References
1- براون، .ل. 1380. آبزی‌پروری برای دامپزشکان، مدیریت پرورش ماهی و بیماری‌ها. ترجمه، رحیم پیغان، مهرداد عبدالله مشایی. انتشارات دانشگاه چمران. 916 ص.
2- جانسون، تد. آر. و کیس، ک.ل. 1381. آزمایش‌های میکروب شناسی عمومی. ترجمه، ناصر گلبانگ (1946). دانشگاه اصفهان. 268 ص.
3- دیبا، ک. 1390. اصول قارچ‌شناسی پزشکی. موسسه فرهنگی انتشاراتی شاهد و ایثارگران دانشگاه علوم پزشکی ارومیه. 218 ص.
4- واکر، گ.م. 1391. مخمر فیزیولوژی و بیوتکنولوژی. ترجمه پیمان پورنیا، رضا کچوئی. انتشارات جعفری. 486 ص.

5. Andlid, T., Vazquez-Juarez, R., and L. Gustafsson. 1995. Yeast colonizing the intestine of rainbow trout, Salmo gairdneri and turbot, Scophthalmus maximus. Microbial Ecology 30: 321–334.
6. Bardócz, S. 1993. The role of dietary polyamines. European Journal of Clinical Nutrition 47: 683-690.
7. Black, J.G. 1999. Microbiology: Principles and Explorations Marymount University. Prentice Hall Books 896 p.
8. Bowen, S., Fogarino E., Hitchner K., Dana G., Victor H.S. Chow, Buoncristiani, M., and J. Carl. 1985. Ecological isolation in Artemia population differences in tolerance of anion concentrations. Journal of Crustacean Biology 5:106-129
9. Coleman, D.C., Sullivan, D.J., Bennett, D.E., Moran, G. P., Barry, H.j., and D.B. Shanley. 1997. Candidiasis: The emergence of a novel species, Candida tropicalis, Candida dubliniensis. AIDS. 11:557-567.
10. Deak, T., Chen, J., and L.R. Beuchat. 2000. Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry. Applied and Environmental Microbiology 66, 4340–4344.
11. Gatesoupe, F.J. 2007. Live yeasts in the gut: Natural occurrence, dietary introduction, and their effects on fish health and development. Aquaculture 267 (1-4) : 20-30.
12. Hazen, K.C. 1995. New and emerging yeast pathogens. Clinical Microbiology, 8,462-478.
13. James, C.M., and B.A. Makkeya. 1981. Production of rotifers, Brachionus plicatilis, brine shrimp, Artemia salina and copepods for aquaculture. Annual research report, Kuwait Institute for Scientific Research: 103-107.
14. Kirkpatrick, W.R., Revankar, S.G., Mcatee, R.K., Lopez-Ribot, J.L., Fothergill, A.W., McCarthy, D.I., Sanche, S.E., Cantu, R.A., Rinaldi, M.G., and T.F. Patterson. 1998. Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates. Journal of Clinical Microbiology 36: 3007-3012.
15. Kwon-Chung, K.J., and J.E. Bennett. 1992. Medical Mycology. Lea and Febiger, Philadelphia.
16. Lachance, M.A. 1990. Yeast selection in nature. In Panchal, C.J. Editor. Yeast Strain Selection. Marcel Dekker pp. 21-42.
17. Larone, D.H. 2002. Medically important fungi. A Guide to identification. ASM Press. 409 p.
18. Lavens, P., and P. Sorgeloos. 1991. Manual on the production and use of live food for aquaculture. FAO Fisheries Technical Paper No. 361.
19. Manaffar, R. 2012. Genetic diversity of Artemia populations in Lake Urmia, Iran. PhD thesis, Ghent University, Belgium.
20. Olmos, J., Paniagua, J., and R. Contreas. 2000. Molecular identification of Dunaliella sp. utilizing the 18SrDNA gene. Letters in Applied Microbiology 30(1) : 80-84.
21. Paritpokee, S., Hall, G., and G. Procop. 2005. Rapid identification of yeast isolates using BD BBLTM CHROMagarTM Candida. Conference presented American society for microbiology.
22. Phaff, H.J., and W.T. Starmer. 1987. Yeasts Associated with Plants, Insect and Soil.Pp.123-180.In: The Yeasts, Vol.1.Eds .A.H. Ros and J.S. Hamilton Acadomic, London.
23. Sambrook, J., Fritsch, E.F., and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd edition, Cold Spring Harbor Laboratory Press, New York.
24. San-Millan, R. Ribacoba, L., Ponton, J., and G. Quindos. 1996. Evaluation of a commercial medium for identification of Candida species. European Journal of clinical microbial infection disease 15:153-158.
25. Spencer, J.F.T., and D.M. Spencer. 1990. Yeast Technology. Springer-Verlag, Berlin.
26. Stones, C.S., and Mills, D.V. 2004. The use of live yeast and yeast culture products in aquaculture. International Aquafeed 7 (5), 28-34.
27. Sullivan, D., and D. Coleman. 1998. Candida dubliniensis: characteristics and identification. Journal of Clin Microbiology 36: 329-334.

 

Statistics
Article View: 1,073
PDF Download: 1,911


counter
Journal management system. designed by sinaweb