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Subtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 71، شماره 2، شهریور 2016، صفحه 97-102 اصل مقاله (230.92 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2000.106447 | ||
| نویسندگان | ||
| N. Golab1؛ N. Khaki* 2؛ F. Noorbakhsh1 | ||
| 1Department of Microbiology, Faculty of Science, Islamin Azad University, Varamin Branch, Varamin, Iran | ||
| 2Department of Microbiology, Razi Vaccine & Serum Research Institute, Karaj, Iran | ||
| چکیده | ||
| Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All 46 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. In this study, PFGE and serotyping were used to subtype 46 Salmonella isolates belonging to 27different serovars and derived from human and different food origins. Among these isolates, S. Typhimurium was found to be the most predominant serovar. 40 PFGE patterns out of 46 isolates were obtained. The Discrimination Index obtained by serotyping (DI = 0.93) was lower than PFGE (DI = 0.99). Subtyping of Salmonella enterica is very important and shows that animal origin can be one of a reservoir that potentially could be transferred to human through the food chain. In addition, results of this study also revealed that this procedure is a golden standard for genotyping of such salmonellaserotypes. | ||
| کلیدواژهها | ||
| PFGE؛ Salmonella enterica؛ serotyping؛ Subtyping؛ Human؛ Food animals | ||
| عنوان مقاله [English] | ||
| شناسایی زیرگونه سالمونلا انتریکا، مطالعه جدا شده از انسان و خوراک دام با استفاده از Pulse-Field الکتروفورز | ||
| نویسندگان [English] | ||
| نرگس گلاب1؛ پژواک خاکی2؛ فاطمه نوربخش1 | ||
| 1گروه میکروب شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد ورامین، ورامین ایران | ||
| 2بخش میکروب شناسی، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران | ||
| چکیده [English] | ||
| آلودگی سالمونلا دومین علت بیماریهای مشترک بین انسان و دام می باشد که با منشا مواد خوراکی قابل سرایت است. منبع اصلی این عفونت در انسان محصولات غذایی آلوده میباشد. هدف از انجام این مطالعه، جداسازی زیرگونه سالمونلا انتریکا میباشد که در مطالعه پیشین توسط تیم تحقیقاتی حاضر و با استفاده از روش الکتروفورز پالس فیلد صورت گرفته است. همه 46 گونه جدایه سالمونلا تعیین سروتیپ شده و سپس مورد آزمون PFGE قرار گرفتند. همه جدایهها با استفاده از روش های مولکولی XbaI PFGE بررسی شدند. در این مطالعه PFGE و سروتایپینگ برای جداسازی 46 زیرگونه سالمونلا که متعلق به 27 سرووار مختلف میباشد، مورد استفاده قرار گرفت. این زیرگونهها با منشاء انسان و منابع غذایی مختلف به دست آمدهاند. در میان این جدایه ها سالمونلا تیفیموریوم به عنوان شایعترین سرووار شناسایی شد. از 46 جدایه، 40 الگوی PFGE بدست آمده است. شاخص تمایز بدست آمده ناشی از سروتایپینگ (DI=0.93) پایینتر از شاخص مربوط به PFGE (DI=0.99) بوده است. تعیین زیرگونه سالمونلا بسیار مهم بوده و نشان دهنده منشا حیوانی است که به عنوان یکی از منابع ذخیرهای این آلودگی قلمداد شده و بالقوه دارای توانایی انتقال به انسان از طریق زنجیره غذایی میباشد. ضمنا نتایج این مطالعه مشخص نمود که این رویه به عنوان یک استاندارد طلایی به منظور تعیین ژنوتیپ سروتیپهای مختلف سالمونلا به حساب میآید. | ||
| کلیدواژهها [English] | ||
| PFGE, سالمونلا انتریکا, سروتایپینگ, تعیین زیرگونه, انسان, دام | ||
| مراجع | ||
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